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Filtering by: Creator Loughborough, W. Karl Remove constraint Creator: Loughborough, W. Karl Advisor Giovannoni, Stephen J. Remove constraint Advisor: Giovannoni, Stephen J. License All rights reserved Remove constraint License: All rights reserved

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  • Marine bacterioplankton abundances and distributions

    Creator:
    Morris, Robert M.
    Abstract:
    Microorganisms play key roles in ocean biogeochemistry. However, several predominant groups of uncultured bacterioplankton thought to contribute to important biogeochemical processes in the oceans are known primarily from gene cloning studies. Although these studies have greatly expanded our view of microbial diversity in the oceans, they are not quantitative and...
    Resource Type:
    Dissertation
    Full Text:
    ' N, 124° 10.6' W) in September 13 Figure 2.1. Distribution of the SARi 1 dade in the world's

  • Microbial Abundance and Community Analysis in Devil’s Hole, Harrington Sound, Bermuda

    Creator:
    Denman, Carmen C.
    Abstract:
    Microbial activity within elevated CO2 and oxygen depleted environments changes with density driven stratification overturn in the seasonally anoxic region of Devil’s Hole, Bermuda. The temperature gradient developed during the summer months creates a natural laboratory to study bacterial and virus population density and microbial community dynamics in the anoxic...
    Resource Type:
    Honors College Thesis
    Full Text:
    (32 N 19.399’, 64 W 43.08’) located within Bermuda’s Harrington Sound was chosen for collection and

  • Proteomic analyses of marine bacteria

    Creator:
    Sowell, Sarah M.
    Abstract:
    Proteins are the metabolic machines of the cell and as such, the study of proteins could illuminate the dominant biological activities that are occurring within cells and reveal how an organism interacts with its environment. Here, we used proteomic techniques to study the abundant marine bacterium SAR11 both as an...
    Resource Type:
    Dissertation

  • Genome-enabled investigation of the minimal growth requirements andphosphate metabolism for Pelagibacter marine bacteria

    Creator:
    Carini, Paul J.
    Abstract:
    Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...
    Resource Type:
    Dissertation
    Full Text:
    previous cultivation difficulties. First, growth was completely inhibited in the presence of 0.001% (w/v

  • Metabolism of one carbon (C1) and methylated compounds in SAR11 bacteria

    Creator:
    Sun, Jing
    Abstract:
    SAR11 Alphaproteobacteria are the most abundant aerobic chemoheterotrophs in ocean surface waters. Previous studies have indicated SAR11 cells play an important role in marine carbon cycling and consume up to half of some common dissolved organic compounds, such as amino acids. During sequencing of the first SAR11 genome, genes for...
    Resource Type:
    Dissertation
    Full Text:
    analyses. Dr. Andrew W B Johnston’s group found the possible DMSP lyase, they cloned, expressed and

  • Microbial diversity, metabolic potential, and transcriptional activity along the inner continental shelf of the Northeast Pacific Ocean

    Creator:
    Bertagnolli, Anthony D.
    Abstract:
    Continental shelves located along eastern boundary currents occupy relatively small volumes of the world’s oceans, yet are responsible for a large proportion of global primary production. The Oregon coast is among these ecosystems. Recent analyses of dissolved oxygen at shallow depths in the water column has suggested increasing episodes of...
    Resource Type:
    Dissertation
    Full Text:
    . 44°15 ’N) at stations SH50 (West Long. 124°10’ W) and SH100 (West Long. 124°27’ W) on the RV Elakha

  • Culturing uncultured environmental microorganisms

    Creator:
    Connon, Stephanie A.
    Abstract:
    Research on natural environments, over the last decade, is replete with microbial diversity studies that used culture-independent approaches. The cloning and sequencing of the 16S rRNA genes has been the driving force in the expansion of awareness about the great diversity of previously undiscovered microorganisms. Well-known uncultured groups of microorganisms...
    Resource Type:
    Dissertation
    Full Text:
    . I., W. Ludwig and K.-H. Schleifer (1995). Phylogenetic identification and in situ detection of

  • Quantification of Osmolytes in the Sargasso Sea Surface Layer Water Column

    Creator:
    Muslin, Omran
    Abstract:
    Oceanic carbon cycling plays a major role in determining global atmospheric CO₂. A better understanding of dissolved organic matter and its constituents in the water column and how it affects marine carbon cycling is sought after. This study successfully measured osmolytes, low molecular zwitterionic compounds derived from amino acids, in...
    Resource Type:
    Masters Thesis
    Full Text:
    September forming the DCM. Adapted from Giovannoni & Vergin 2012. (31°40’N, 64°10’W), that DOC is most

  • The proteomic and transcriptomic responses to iron, sulfur, and nitrogen limitation in the abundant marine bacterium Candidatus Pelagibacter ubique

    Creator:
    Smith, Daniel P.
    Abstract:
    Batch cultures of Candidatus Pelagibacter ubique were grown under iron-, organosulfur-, and nitrogen-limiting conditions to understand how this ubiquitous marine bacterium responds to and interacts with environments where growth is limited by the availability of these nutrients. Global gene expression was monitored using microarrays and quantitative mass spectrometry to observe...
    Resource Type:
    Dissertation

  • Single-Cell Genomics of Uncultivated Marine Bacteria

    Creator:
    Landry, Zachary
    Abstract:
    Historically, the difficulty of obtaining pure cultures of abundant marine microbial plankton has an obstacle to reconstructing the underlying mechanisms of biogeochemistry in the ocean. While a number of dominant marine species from the ocean surface have been cultured, the dominant microbial plankton of the dark ocean proved far more...
    Resource Type:
    Dissertation