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Filtering by: Creator Mercer, Dacey M. Remove constraint Creator: Mercer, Dacey M. Advisor Giovannoni, Stephen J. Remove constraint Advisor: Giovannoni, Stephen J.

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  • Proteomic analyses of marine bacteria

    Creator:
    Sowell, Sarah M.
    Abstract:
    Proteins are the metabolic machines of the cell and as such, the study of proteins could illuminate the dominant biological activities that are occurring within cells and reveal how an organism interacts with its environment. Here, we used proteomic techniques to study the abundant marine bacterium SAR11 both as an...
    Resource Type:
    Dissertation
    Full Text:
    THE DISSERTATION OF Sarah M. Sowell for the degree of Doctor of Philosophy in Molecular and

  • Quantification of Osmolytes in the Sargasso Sea Surface Layer Water Column

    Creator:
    Muslin, Omran
    Abstract:
    Oceanic carbon cycling plays a major role in determining global atmospheric CO₂. A better understanding of dissolved organic matter and its constituents in the water column and how it affects marine carbon cycling is sought after. This study successfully measured osmolytes, low molecular zwitterionic compounds derived from amino acids, in...
    Resource Type:
    Masters Thesis
    Full Text:
    weighed. This pellet is then re-suspended in nanopure water, water with18.2 MΩ ionic purity and little

  • Genomic-assisted determination of the natural nutrient requirements of the cosmopolitan marine bacterium 'Candidatus Pelagibacter ubique'

    Creator:
    Tripp, H. James
    Abstract:
    To identify factors limiting 'Candidatus Pelagibacter ubique' maximum cell density and growth rate in pure culture on seawater, the genome sequence of 'Cand. P. ubique' was analyzed, culturing experiments with organic and inorganic nutrient additions were made, and radiotracer uptake experiments were performed. The genome was sequenced, custom data mining...
    Resource Type:
    Dissertation
    Full Text:
    cell yield increased an order of magnitude. But, increasing DMSP additions from 50 nM to 10 μM did

  • Diversity of bacterioplankton and plastid SSU rRNA genes from the eastern and western continental shelves of the United States

    Creator:
    Rappé, Michael Stephen
    Abstract:
    The phylogenetic diversity of two continental shelf picoplankton communities was examined by analyzing SSU (16S) ribosomal RNA (rRNA) genes amplified from environmental DNA with bacterial-specific primers and the polymerase chain reaction (PCR). Picoplankton populations collected from the pycnocline (10 m) over the eastern continental shelf of the United States near...
    Resource Type:
    Dissertation
    Full Text:
    collected from the pycnocline (10 m) over the eastern continental shelf of the United States near Cape

  • Characterizing Growth Promoters and Inhibitors of SAR11 Pelagibacter sp. HTCC7211

    Creator:
    Lee, Connie
    Abstract:
    SAR11 is a clade of marine bacteria that are the most abundant heterotrophs in ocean surface water. They play a significant role in marine carbon cycling. Although many culturing experiments have been done on SAR11, there was no research that focused on verifying the observations about inhibition of SAR11 growth...
    Resource Type:
    Honors College Thesis
    Full Text:
    autoclaved nanopure water was sparged with 0.1 μM-filtered CO2 for at least 6 hours, followed by sparging

  • Single-Cell Genomics of Uncultivated Marine Bacteria

    Creator:
    Landry, Zachary
    Abstract:
    Historically, the difficulty of obtaining pure cultures of abundant marine microbial plankton has an obstacle to reconstructing the underlying mechanisms of biogeochemistry in the ocean. While a number of dominant marine species from the ocean surface have been cultured, the dominant microbial plankton of the dark ocean proved far more...
    Resource Type:
    Dissertation
    Full Text:
    ., Thrash, J.C., Schwalbach, M.S., Vergin, K.L., Landry, Z.C., Ellisman, M., Deerinck, T., Sullivan, M.B

  • Metabolism of one carbon (C1) and methylated compounds in SAR11 bacteria

    Creator:
    Sun, Jing
    Abstract:
    SAR11 Alphaproteobacteria are the most abundant aerobic chemoheterotrophs in ocean surface waters. Previous studies have indicated SAR11 cells play an important role in marine carbon cycling and consume up to half of some common dissolved organic compounds, such as amino acids. During sequencing of the first SAR11 genome, genes for...
    Resource Type:
    Dissertation
    Full Text:
    concentration in deeper water (~500 m) remained significantly high, suggesting that its production is not

  • Microbial Abundance and Community Analysis in Devil’s Hole, Harrington Sound, Bermuda

    Creator:
    Denman, Carmen C.
    Abstract:
    Microbial activity within elevated CO2 and oxygen depleted environments changes with density driven stratification overturn in the seasonally anoxic region of Devil’s Hole, Bermuda. The temperature gradient developed during the summer months creates a natural laboratory to study bacterial and virus population density and microbial community dynamics in the anoxic...
    Resource Type:
    Honors College Thesis
    Full Text:
    40 µl of 2ng/µl probe in hybridization solution (0.9 M NaCl, appropriate % formamide as detailed

  • Microbial Abundance and Community Analysis in Devil’s Hole, Harrington Sound, Bermuda

    Creator:
    Denman, Carmen C.
    Abstract:
    Microbial activity within elevated CO2 and oxygen depleted environments changes with density driven stratification overturn in the seasonally anoxic region of Devil’s Hole, Bermuda. The temperature gradient developed during the summer months creates a natural laboratory to study bacterial and virus population density and microbial community dynamics in the anoxic...
    Resource Type:
    Honors College Thesis
    Full Text:
    40 µl of 2ng/µl probe in hybridization solution (0.9 M NaCl, appropriate % formamide as detailed

  • The proteomic and transcriptomic responses to iron, sulfur, and nitrogen limitation in the abundant marine bacterium Candidatus Pelagibacter ubique

    Creator:
    Smith, Daniel P.
    Abstract:
    Batch cultures of Candidatus Pelagibacter ubique were grown under iron-, organosulfur-, and nitrogen-limiting conditions to understand how this ubiquitous marine bacterium responds to and interacts with environments where growth is limited by the availability of these nutrients. Global gene expression was monitored using microarrays and quantitative mass spectrometry to observe...
    Resource Type:
    Dissertation
    Full Text:
    Trichodesmium [37] accounted for the majority of N2 fixation, with a maximum observed rate of ~200 µmol N m-2