Index Catalog // ScholarsArchive@OSU

A genomic and proteomic characterization of the first cultured oligotrophic marine Gammaproteobacterium from the SAR92 clade

Creator:: Mellbye, Brett L.
Abstract:: High-throughput culturing (HTC) consisting of extinction culturing in autoclaved seawater has led to the isolation and characterization of many novel strains of oligotrophic marine bacteria. Strain HTCC 2207 was isolated from the Oregon coast by the HTC method. Phylogenetic analysis based on 16S rRNA gene sequence showed that this strain...
Resource Type:: Honors College Thesis
Full Text:: 2390 open reading frames. The G+C content is 49.10 mol %. The bacterium possesses all major metabolic

Characterization of novel marine oligotrophic bacteria isolated from the Pacific Ocean : description of Marinivirgula fluito gen. nov., sp. nov., Marinivirgula obesa gen. nov., sp. nov. and Litincola parvulus gen. nov., sp. nov.

Creator:: Shin, Eun Jung
Abstract:: A new high-throughput culturing (HTC) method using a low nutrient heterotrophic medium (LNHM) has led to the isolation of many novel strains of oligotrophic bacteria from marine ecosystems. Four strains belonging to a single dade, HTCC2151, HTCC218OT, HTCC2178T and HTCC2188T, were isolated from the coast of Oregon by the HTC...
Resource Type:: Masters Thesis
Full Text:: , and thus it was proposed that strain HTCC2188T be placed in a separate genus in the 0M182 dade. G+C

An analysis of Sargasso Sea bacterioplankton diversity using 16S ribosomal RNA

Creator:: Britschgi, Theresa Baden
Abstract:: The objective of this project was to use ribosomal RNA genes, cloned from natural populations of Sargasso Sea bacterioplankton, as markers for picoplankton diversity. It is widely recognised that a majority of microorganisms have yet to be cultivated, and therefore much of extant microbial diversity remains unknown (50). The method...
Resource Type:: Masters Thesis
Full Text:: surroundings using nucleic acid hybridization techniques. A GCCe C U U G A G G C CG A U U 0' A A G CG U A

Arsenic methylation by marine cyanobacteria Prochlorococcus and Synechococcus

Creator:: Lee, Mikaela
Abstract:: Our experiments aimed to investigate responses of the marine cyanobacteria Prochlorococcus and Synechococcus to toxic arsenic species, such as arsenate, and to understand cellular mechanisms that contribute to arsenic tolerance. We used two strains of Prochlorococcus, a high-light adapted MED4 and low-light adapted MIT9313, both axenic, for cultivation experiments. For...
Resource Type:: Honors College Thesis
Full Text:: and b. Arch Microbiol 157:297-300 doi:10.1007/BF00245165. 22. Moore LR, Rocap G, Chisholm SW

Proteomic analyses of marine bacteria

Creator:: Sowell, Sarah M.
Abstract:: Proteins are the metabolic machines of the cell and as such, the study of proteins could illuminate the dominant biological activities that are occurring within cells and reveal how an organism interacts with its environment. Here, we used proteomic techniques to study the abundant marine bacterium SAR11 both as an...
Resource Type:: Dissertation
Full Text:: Microbiology Initiative investigator award from the Gordon and Betty Moore Foundation. Portions of this

Diversity of bacterioplankton and plastid SSU rRNA genes from the eastern and western continental shelves of the United States

Creator:: Rappé, Michael Stephen
Abstract:: The phylogenetic diversity of two continental shelf picoplankton communities was examined by analyzing SSU (16S) ribosomal RNA (rRNA) genes amplified from environmental DNA with bacterial-specific primers and the polymerase chain reaction (PCR). Picoplankton populations collected from the pycnocline (10 m) over the eastern continental shelf of the United States near...
Resource Type:: Dissertation
Full Text:: with other members of the high G + C Gram-positive phylum 78 3.6 Proposed secondary structural model

Prokaryotes associated with marine crust

Creator:: Mason, Olivia Underwood
Abstract:: Oceanic crust covers nearly 70% of the Earth's surface, of which, the upper, sediment layer is estimated to harbor substantial microbial biomass. Marine crust; however, extends several kilometers beyond this surficial layer, and includes the basalt and gabbro layers. In particular, the basalt layer has high permeabilities which allows for...
Resource Type:: Dissertation
Full Text:: quantitative analyses revealed that basalts harbor 6 × 105 to 4 × 106 and 3 × 106 to 1 × 109 cells g-1 (Einen

Characterizing Growth Promoters and Inhibitors of SAR11 Pelagibacter sp. HTCC7211

Creator:: Lee, Connie
Abstract:: SAR11 is a clade of marine bacteria that are the most abundant heterotrophs in ocean surface water. They play a significant role in marine carbon cycling. Although many culturing experiments have been done on SAR11, there was no research that focused on verifying the observations about inhibition of SAR11 growth...
Resource Type:: Honors College Thesis
Full Text:: g ro w th r a te ( d a y -1 ) Concentration (µM) Oxaloacetate Taurine DMSP Betaine

Disturbances in a marine bacterioplankton system drive community assembly towards niche-based processes

Creator:: Vergin, Kevin L.
Abstract:: Over 100 monthly bacterioplankton DNA samples, from each of the surface and 200 m depths at the Bermuda Atlantic Time-series Study (BATS) site, were analyzed for community assembly processes. Correlation networks, filtered for potential autocorrelation artifacts, were constructed for each depth. Network characteristics for the two depths were remarkably similar...
Resource Type:: Masters Thesis
Full Text:: NSF Microbial Observatory grants and an investigator award from the Gordon and Betty Moore

Microbial Abundance and Community Analysis in Devil’s Hole, Harrington Sound, Bermuda

Creator:: Denman, Carmen C.
Abstract:: Microbial activity within elevated CO2 and oxygen depleted environments changes with density driven stratification overturn in the seasonally anoxic region of Devil’s Hole, Bermuda. The temperature gradient developed during the summer months creates a natural laboratory to study bacterial and virus population density and microbial community dynamics in the anoxic...
Resource Type:: Honors College Thesis
Full Text:: Institute of Ocean Sciences, the Gordon and Betty Moore Foundation, and the honors excellence fund

ScholarsArchive@OSU

A genomic and proteomic characterization of the first cultured oligotrophic marine Gammaproteobacterium from the SAR92 clade

Characterization of novel marine oligotrophic bacteria isolated from the Pacific Ocean : description of Marinivirgula fluito gen. nov., sp. nov., Marinivirgula obesa gen. nov., sp. nov. and Litincola parvulus gen. nov., sp. nov.

An analysis of Sargasso Sea bacterioplankton diversity using 16S ribosomal RNA

Arsenic methylation by marine cyanobacteria Prochlorococcus and Synechococcus

Proteomic analyses of marine bacteria

Diversity of bacterioplankton and plastid SSU rRNA genes from the eastern and western continental shelves of the United States

Prokaryotes associated with marine crust

Characterizing Growth Promoters and Inhibitors of SAR11 Pelagibacter sp. HTCC7211

Disturbances in a marine bacterioplankton system drive community assembly towards niche-based processes

Microbial Abundance and Community Analysis in Devil’s Hole, Harrington Sound, Bermuda

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