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Creator:: Connon, Stephanie A.
Abstract:: Research on natural environments, over the last decade, is replete with microbial diversity studies that used culture-independent approaches. The cloning and sequencing of the 16S rRNA genes has been the driving force in the expansion of awareness about the great diversity of previously undiscovered microorganisms. Well-known uncultured groups of microorganisms...
Resource Type:: Dissertation
Full Text:: 0.2 jim black polycarbonate membranes for counting ............ 69 3.3 The LH-PCR electropherograms

Creator:: Shin, Eun Jung
Abstract:: A new high-throughput culturing (HTC) method using a low nutrient heterotrophic medium (LNHM) has led to the isolation of many novel strains of oligotrophic bacteria from marine ecosystems. Four strains belonging to a single dade, HTCC2151, HTCC218OT, HTCC2178T and HTCC2188T, were isolated from the coast of Oregon by the HTC...
Resource Type:: Masters Thesis
Full Text:: tM NH4C1 and 0.1 jiM K}12PO4. Cell cultures were inoculated at final cell densities of 1 O3cells/ml

Creator:: Smith, Daniel P.
Abstract:: Batch cultures of Candidatus Pelagibacter ubique were grown under iron-, organosulfur-, and nitrogen-limiting conditions to understand how this ubiquitous marine bacterium responds to and interacts with environments where growth is limited by the availability of these nutrients. Global gene expression was monitored using microarrays and quantitative mass spectrometry to observe...
Resource Type:: Dissertation
Full Text:: to artificial seawater. Nutrient mixes progressively refined by Kevin Vergin, Josh Kitner, Jim

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