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Filtering by: Creator Sullivan, Angelique S. Remove constraint Creator: Sullivan, Angelique S. Advisor Giovannoni, Stephen J. Remove constraint Advisor: Giovannoni, Stephen J.

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  • Metabolism of one carbon (C1) and methylated compounds in SAR11 bacteria

    Creator:
    Sun, Jing
    Abstract:
    SAR11 Alphaproteobacteria are the most abundant aerobic chemoheterotrophs in ocean surface waters. Previous studies have indicated SAR11 cells play an important role in marine carbon cycling and consume up to half of some common dissolved organic compounds, such as amino acids. During sequencing of the first SAR11 genome, genes for...
    Resource Type:
    Dissertation
    Full Text:
    would also like to thank my supervisory committee members, Dr. Daniel D. Rockey, Dr. Joseph S. Beckman

  • Disturbances in a marine bacterioplankton system drive community assembly towards niche-based processes

    Creator:
    Vergin, Kevin L.
    Abstract:
    Over 100 monthly bacterioplankton DNA samples, from each of the surface and 200 m depths at the Bermuda Atlantic Time-series Study (BATS) site, were analyzed for community assembly processes. Correlation networks, filtered for potential autocorrelation artifacts, were constructed for each depth. Network characteristics for the two depths were remarkably similar...
    Resource Type:
    Masters Thesis
    Full Text:
    after the missing sample(s). Simulations demonstrate that non-normality in the residuals of only one

  • Microbial diversity, metabolic potential, and transcriptional activity along the inner continental shelf of the Northeast Pacific Ocean

    Creator:
    Bertagnolli, Anthony D.
    Abstract:
    Continental shelves located along eastern boundary currents occupy relatively small volumes of the world’s oceans, yet are responsible for a large proportion of global primary production. The Oregon coast is among these ecosystems. Recent analyses of dissolved oxygen at shallow depths in the water column has suggested increasing episodes of...
    Resource Type:
    Dissertation

  • Characterizing Growth Promoters and Inhibitors of SAR11 Pelagibacter sp. HTCC7211

    Creator:
    Lee, Connie
    Abstract:
    SAR11 is a clade of marine bacteria that are the most abundant heterotrophs in ocean surface water. They play a significant role in marine carbon cycling. Although many culturing experiments have been done on SAR11, there was no research that focused on verifying the observations about inhibition of SAR11 growth...
    Resource Type:
    Honors College Thesis
    Full Text:
    help understand SAR11’s role in nutrient cycling in marine ecosystems. Key Words: SAR11

  • The proteomic and transcriptomic responses to iron, sulfur, and nitrogen limitation in the abundant marine bacterium Candidatus Pelagibacter ubique

    Creator:
    Smith, Daniel P.
    Abstract:
    Batch cultures of Candidatus Pelagibacter ubique were grown under iron-, organosulfur-, and nitrogen-limiting conditions to understand how this ubiquitous marine bacterium responds to and interacts with environments where growth is limited by the availability of these nutrients. Global gene expression was monitored using microarrays and quantitative mass spectrometry to observe...
    Resource Type:
    Dissertation
    Full Text:
    , whereas organosulfur-limited cultures were not. Methionine synthesis genes downstream of S-adenosyl

  • Arsenic methylation by marine cyanobacteria Prochlorococcus and Synechococcus

    Creator:
    Lee, Mikaela
    Abstract:
    Our experiments aimed to investigate responses of the marine cyanobacteria Prochlorococcus and Synechococcus to toxic arsenic species, such as arsenate, and to understand cellular mechanisms that contribute to arsenic tolerance. We used two strains of Prochlorococcus, a high-light adapted MED4 and low-light adapted MIT9313, both axenic, for cultivation experiments. For...
    Resource Type:
    Honors College Thesis
    Full Text:
    WH8102 were grown at a light level of ~50-55 μEin/m2/s, and MIT9313ax were grown at a light level of

  • Single-Cell Genomics of Uncultivated Marine Bacteria

    Creator:
    Landry, Zachary
    Abstract:
    Historically, the difficulty of obtaining pure cultures of abundant marine microbial plankton has an obstacle to reconstructing the underlying mechanisms of biogeochemistry in the ocean. While a number of dominant marine species from the ocean surface have been cultured, the dominant microbial plankton of the dark ocean proved far more...
    Resource Type:
    Dissertation
    Full Text:
    ., Thrash, J.C., Schwalbach, M.S., Vergin, K.L., Landry, Z.C., Ellisman, M., Deerinck, T., Sullivan, M.B

  • Proteomic analyses of marine bacteria

    Creator:
    Sowell, Sarah M.
    Abstract:
    Proteins are the metabolic machines of the cell and as such, the study of proteins could illuminate the dominant biological activities that are occurring within cells and reveal how an organism interacts with its environment. Here, we used proteomic techniques to study the abundant marine bacterium SAR11 both as an...
    Resource Type:
    Dissertation
    Full Text:
    research were also supported by the U. S. Department of Energy (DOE) Office of Biological and

  • Conserved properties in the metagenome of a large bacterioplankton population

    Creator:
    Wilhelm, Larry John
    Abstract:
    Candidatus Pelagibacter ubique is the first cultured representative of the SAR11 clade, a clade that is found throughout the oceans and accounts for approximately 25% of all bacterial cells [1]. It has a streamlined genome that is the smallest of any known free-living organism. In this study the complete genome...
    Resource Type:
    Masters Thesis
    Full Text:
    4 16 0 16 6 17 2 17 8 -log e-value N um be r o f S yn tig s

  • Genome-enabled investigation of the minimal growth requirements andphosphate metabolism for Pelagibacter marine bacteria

    Creator:
    Carini, Paul J.
    Abstract:
    Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...
    Resource Type:
    Dissertation
    Full Text:
    . ......................................................................................... 149 Figure A2- 6: Profiles of in situ temperature and chlorophyll a fluorescence at Hydrostation S