Search Constraints

Start Over
Filtering by: Creator Garity, Dennis J. Remove constraint Creator: Garity, Dennis J. Advisor Hruby, Dennis E. Remove constraint Advisor: Hruby, Dennis E.

Search Results

  • Structure-function analysis of the vaccinia virus I7L proteinase

    Creator:
    Moerdyk, Megan J.
    Abstract:
    Vaccinia virus is the prototypic member of the Orthopoxvirus genus. It undergoes a complex replication process where a key step in the transition from immature virion to intracellular mature virion is the cleavage of the major core protein precursors. The product of the I7L open reading frame (ORF) is a...
    Resource Type:
    Masters Thesis
    Full Text:
    OF Megan J. Moerdyk for the degree of Master of Science in Microbiology presented on December

  • Evidence for the modification of vaccinia virus core proteins by ADP-ribosylation

    Creator:
    Child, Stephanie J.
    Abstract:
    Vaccinia virus (VV), the prototype member of the orthopoxvirus family, is a large virus of complex morphology which contains a 191 Kbp double-stranded DNA genome whose expression is tightly regulated in a temporal fashion during viral replication. The regulation of gene expression can be exerted at various of levels, including...
    Resource Type:
    Dissertation
    Full Text:
    ADP-RIBOSYLATION OF VIRAL PROTEINS Stephanie J. Child, Christine A. Franke, and Dennis E. Hruby 16

  • Vaccinia virus I7L core protein proteinase

    Creator:
    Byrd, Chelsea M.
    Abstract:
    Vaccinia virus (VV) is a large double-stranded DNA virus that is a prototypic member of the orthopoxvirus family. Previous works has showed that three of the major structural proteins found within the mature VV virion core 4a, 4b, and 25K are produced from higher molecular weight precursors at late times...
    Resource Type:
    Dissertation
    Full Text:
    : Vaccinia Virus 17L Core Protein Proteinase Abstract approved: Dr. Dennis E. Vaccinia virus (VV) is a

  • Characterization of vaccinia virus A12L protein : its proteolysis and functional analyses in virus replication

    Creator:
    Yang, Su Jung
    Abstract:
    In order to produce infectious virus progeny, vaccinia virus (VV) undergoes morphogenic proteolysis to regulate the structural rearrangements of virus particles. Several of the major structural precursor proteins of VV are cleaved at a conserved Ala-Gly-X (where X is any amino acid) motif by the VV I7L core protein proteinase...
    Resource Type:
    Dissertation
    Full Text:
    : Dr. Dennis E. Hruby In order to produce infectious virus progeny, vaccinia virus (VV

  • Molecular genetic analysis of vaccinia virus genes which confer resistance to alpha-amanitin

    Creator:
    Tamin, Azaibi
    Abstract:
    Several laboratories have shown that the VV replication cycle is dependent on the host cell nucleus. The basic biological observation is that if the host cell nucleus is physically removed by cytochalasin B-mediated enucleation, functionally inactivated by UV-irradiation, or selectively blocked with a-amanitin, the results are the same: W is...
    Resource Type:
    Dissertation
    Full Text:
    Dr. Dennis E. Hruby Several laboratories have shown that the VV replication cycle is dependent on

  • Palmitylation of vaccinia virus proteins : identification of modification sites and biological relevance

    Creator:
    Grosenbach, Douglas W.
    Abstract:
    Vaccinia virus encodes at least eight proteins that are modified post-translationally by the addition of a 16-carbon saturated fatty acid through thioester linkage to cysteine residues. This is referred to as palmitylation of proteins. The purpose of this work was to gain an understanding of palmitylation, focusing on what defined...
    Resource Type:
    Dissertation
    Full Text:
    as well. I am grateful to Dennis Hruby not only for advising me through this course of study, but

  • Fatty acylation of Vaccinia virus proteins : dual myristylation and palmitylation of the A-type inclusion protein

    Creator:
    Martin, Karen H.
    Abstract:
    The attachment of myristic acid to the N-terminal glycine residue of many eukaryotic and viral proteins is often essential for the acquisition of the protein's biological activity. Vaccinia virus (VV), the prototype member of the Poxviridae, expresses several myristylated proteins during the course of infection. Only one of these proteins,...
    Resource Type:
    Dissertation
    Full Text:
    . Abstract approved: Dennis E. Hruby The attachment of myristic acid to the N-terminal glycine residue of

  • Regulation of late gene expression in vaccinia virus

    Creator:
    Weinrich, Scott L.
    Abstract:
    The prototypal poxvirus vaccinia is a cytoplasmic-replicating DNA virus which contains a large genome with the capacity to encode 200 polypeptides. Tight temporal regulation coordinates the expression of this large number of genes throughout a relatively short replication cycle. As a first step towards identifying and understanding the regulatory mechanisms...
    Resource Type:
    Dissertation
    Full Text:
    approved: Redacted for Privacy Dr. Dennis E. Hrull:___ The prototypal poxvirus vaccinia is a cytoplasmic

  • Disulphide bond formation is essential for vaccinia virus late protein L1R function and for production of infectious progeny

    Creator:
    Blouch, Robert Edward
    Abstract:
    L1R, a myristylated late gene product of vaccinia virus, is essential for formation of infectious intracellular mature virions (IMV). In its absence, only viral particles arrested at an immature stage are detected and no infectious progeny virus are produced. Previous studies have shown that the L1R protein is exclusively associated...
    Resource Type:
    Masters Thesis
    Full Text:
    I would like to express my sincere appreciation to Dennis E. Hruby for his patience and guidance

  • The regulation of a vaccinia virus late gene required for morphogenesis

    Creator:
    Miner, Jeffery N.
    Abstract:
    Vaccinia virus gene expression is characterized by tight temporal regulation. Viral early transcription begins within the cytoplasm upon infection; three hours later, early genes are repressed, DNA replication occurs and late gene expression commences. As an approach toward understanding the mechanisms governing vaccinia late gene expression, I have undertaken an...
    Resource Type:
    Dissertation
    Full Text:
    Morphogenesis Abstract approved: Redacted for Priv/ ac)/ Dr. Dennis E. Hruby Vaccinia virus gene expression is