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Vaccinia virus I7L core protein proteinase

Creator:: Byrd, Chelsea M.
Abstract:: Vaccinia virus (VV) is a large double-stranded DNA virus that is a prototypic member of the orthopoxvirus family. Previous works has showed that three of the major structural proteins found within the mature VV virion core 4a, 4b, and 25K are produced from higher molecular weight precursors at late times...
Resource Type:: Dissertation
Full Text:: the viral mRNA. a4a a-4b (x-SK a.L6 P C P C P C P C Figure 1.2. Morphogenic cleavage demonstrated

Structural and functional dissection of the vaccinia virus thymidine kinase enzyme

Creator:: Black, Margaret E.
Abstract:: Thymidine kinase is a key enzyme in the nucleotide salvage pathway, catalyzing the production of dTMP from thymidine and ATP. In order to identify the structural features of the TK protein and/or primary amino acid sequences which contribute to the catalytic and regulatory activities of this enzyme, an in vitro...
Resource Type:: Dissertation
Full Text:: rpm for 20 hours at 4 °C. Determination of molecular weight by gel filtration. A standard curve of

Proteolytic maturation of vaccinia virus virion-associated proteins : analysis of substrate determinants

Creator:: Lee, Peiyu
Resource Type:: Dissertation
Full Text:: Weber, 1985). This step is shown to be inhibited by glycosylation inhibitors (Payne and Kristensson

Proteolytic maturation of vaccinia virus structural proteins

Creator:: VanSlyke, Judy K.
Abstract:: Vaccinia virus (VV) is a large DNA virus belonging to the Orthopoxvirus family. The viral replicative life cycle takes place solely within the cytoplasm of a mammalian host cell. The VV genome contains 196 open reading frames which are expressed in a highly regulated and temporal fashion in order to...
Resource Type:: Dissertation
Full Text:: infection. M C N IC F I I i 1 25 1.5 4.5 19 2.2 P E 1 G LJ IiIt I I I 15 .4 7.9 8.8 4.15.0 8.8 16 0.3

Post-translational modifications of vaccinia virus proteins : molecular analysis of the myristylated L1R gene produc

Creator:: Ravanello, Monica P. G.
Resource Type:: Dissertation
Full Text:: Protein - C - Ethanolamine - P Glycan Inositol PR R1, R2 , alkyl/acyl groups; P, phosphate 2

Palmitylation of vaccinia virus proteins : identification of modification sites and biological relevance

Creator:: Grosenbach, Douglas W.
Abstract:: Vaccinia virus encodes at least eight proteins that are modified post-translationally by the addition of a 16-carbon saturated fatty acid through thioester linkage to cysteine residues. This is referred to as palmitylation of proteins. The purpose of this work was to gain an understanding of palmitylation, focusing on what defined...
Resource Type:: Dissertation
Full Text:: Commencement June 1999 C Doctor of Philosophy thesis of Douglas W. Grosenbach presented on April 29

Investigation of the roles of viral proteinases in the vaccinia virus life cycle

Creator:: Page, Jessica M.
Abstract:: An inducible, mutant virus, designated vvtetO:I7L/G1L, was used to study the morphogenic proteolysis step of the vaccinia virus life cycle. The vvtetO:I7L/G1L controlled the expression of two genes, I7L, a cysteine proteinase, and G1L, a putative metalloproteinase. These proteins are involved in the maturation of viral core proteins, p4a, p4b,...
Resource Type:: Honors College Thesis
Full Text:: hydrolyse single amino acids from the N’ or C’ termini while proteinases cleave between specific amino acids

Identification and analysis of vaccinia virus acylproteins

Creator:: Hansen, Scott G.
Abstract:: Vaccinia virus (VV) encodes at least six proteins that are modified by the addition of a 14-carbon saturated fatty acid through an amide linkage and at least eight proteins that are modified post-translationally by the addition of a 16-carbon saturated fatty acid through linkage to cysteine residues. These post-translational modifications...
Resource Type:: Dissertation
Full Text:: .................................................................... 115 4.3 Construction of glycosylated and c-Myc expressing L1R ..................... 117 4.4

Host cell nuclear involvement in vaccinia virus replication

Creator:: Villarreal, Elcira C.
Abstract:: It was previously shown that vaccinia virus is unable to complete its replicative cycle in cells that have been subjected to cytochalasin-B mediated enucleation prior to infection. Likewise, in the presence of the drug α-amanitin, a potent inhibitor of host RNA polymerase II but not of viral transcription, the replication...
Resource Type:: Dissertation
Full Text:: AN ABSTRACT OF THE THESIS OF Elcira C. Villarreal for the degree of Doctor of Philosophy in

Fatty acylation of Vaccinia virus proteins : dual myristylation and palmitylation of the A-type inclusion protein

Creator:: Martin, Karen H.
Abstract:: The attachment of myristic acid to the N-terminal glycine residue of many eukaryotic and viral proteins is often essential for the acquisition of the protein's biological activity. Vaccinia virus (VV), the prototype member of the Poxviridae, expresses several myristylated proteins during the course of infection. Only one of these proteins,...
Resource Type:: Dissertation
Full Text:: protein did not fit the consensus sequence for N-myristylation (M-G-X-X-X- S/T/A/C/N), suggesting that it

ScholarsArchive@OSU

Vaccinia virus I7L core protein proteinase

Structural and functional dissection of the vaccinia virus thymidine kinase enzyme

Proteolytic maturation of vaccinia virus virion-associated proteins : analysis of substrate determinants

Proteolytic maturation of vaccinia virus structural proteins

Post-translational modifications of vaccinia virus proteins : molecular analysis of the myristylated L1R gene produc

Palmitylation of vaccinia virus proteins : identification of modification sites and biological relevance

Investigation of the roles of viral proteinases in the vaccinia virus life cycle

Identification and analysis of vaccinia virus acylproteins

Host cell nuclear involvement in vaccinia virus replication

Fatty acylation of Vaccinia virus proteins : dual myristylation and palmitylation of the A-type inclusion protein

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