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Creator:: McKenzie, Raymond Leslie
Abstract:: The interaction of polylysine with DNA may be divided into two separate phenomena: the primary interaction involved in formation of a well-defined complex, and secondary interactions leading to aggregation of these complexes. Most studies on the interaction of polylysine with DNA have used techniques which measure properties of the aggregated...
Resource Type:: Dissertation
Full Text:: INTERACTION OF POLYLYSINE WITH DEOXYRIBONUCLEIC ACID Abstract approved:( Redacted for Privacy Dr. Irvin

Creator:: Weber, Shane Crawford
Abstract:: I examined, in three separate studies, the active chromatin structure of the yeast, Saccharomyces cerevisiae. Yeast contains four proteins having amino acid compositions typical of the high mobility group (HMG) proteins. Three of these are eluted from chromatin by 0.35 M NaCl; one is not, but is eluted by 0.25...
Resource Type:: Dissertation
Full Text:: Irvin Isenberg I examined, in three separate studies, the active chromatin structure of the yeast

Creator:: Mardian, James Kenneth Walker
Abstract:: In this work, the four yeast inner histones were prepared by the use of consecutive slab gel fractionation. The use of this technique allowed the first complete fractionation and rigorous identification of the four yeast inner histones, and made possible a study of their complexing behavior. Variants and adjuncts of...
Resource Type:: Dissertation
Full Text:: Walker Mardian ACKNOWLEDGEMENTS I wish to express my profound thanks to Dr. Irvin Isenberg for his

Creator:: Wickett, Richard Randall
Abstract:: The addition of salts or nucleoside triphosphates to histone IV solutions induces conformational changes in the histone molecule. These conformational changes are investigated by measurement of the fluorescence anisotropy of the histone tyrosine emission, the circular dichroism (CD) of the peptide bond and of the tyrosine CD. Changes induced by...
Resource Type:: Dissertation
Full Text:: : Redacted for privacy Irvin Isenburg The addition of salts or nucleoside triphosphates to histone IV

Creator:: Evett, Jay Fredrick
Abstract:: A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl chloride (dansyl chloride), was covalently bonded to polylysine. The dansyl tagged polylysine was complexed to DNA by gradient dialysis. The polarization and intensity of fluorescence of the dansyl and the turbidity of the complex were measured as a function of salt concentration for a variety of...
Resource Type:: Dissertation
Full Text:: instrument built by Spencer L. Baird, Jr. and Irvin Isenberg. The instrument is a continuously recording

Creator:: Szent-Györgyi, Christopher Stephan
Abstract:: I have investigated nucleoprotein organization in chromatin of the yeast, Saccharomyces cerevisiae. My approach has been to prepare insoluble, minimally per turbed chromatin from a mitochondrial DNA-free strain of yeast, using a number of new techniques, and then digest this substrate with micrococcal nuclease. The soluble products are electrophoresed on...
Resource Type:: Dissertation
Full Text:: ACKNOWLEDGEMENT I wish to thank Dr. Irvin Isenberg for expert guid- ance in the ways of science, as an

Creator:: Schuyler, Robert John
Abstract:: In the determination of binding heterogeneity and rotational diffusion relaxation times, fluorescence decay curves often take the form of sums of exponential decay times. An instrument is described which includes features permitting the collection of high precision time decay data, which may successfully be resolved into component decays. The instrument...
Resource Type:: Dissertation
Full Text:: Abstract approved: Irvin Isenberg (j In the determination of binding heterogeneity and rotational

Creator:: Smerdon, Michael John
Abstract:: This work represents the first study of the conformational changes in histone H1 subfractions as well as the interactions of these subfractions with two nonhistone chromosomal (NHC) proteins. Calf thymus Hi was fractionated by the method of Kincade and Cole (1966a) using a very shallow guanidinium chloride gradient. A possible...
Resource Type:: Dissertation
Full Text:: Privacy Abstract approved: Irvin Fsenberg This work represents the first study of the conformational

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