Index Catalog // ScholarsArchive@OSU

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

Creator:: Song, Shiwei
Abstract:: It is well known that the mitochondrial genome has a much higher spontaneous mutation rate than the nuclear genome. mtDNA mutations have been identified in association with many diseases and aging. mtDNA replication continues throughout the cell cycle, even in post-mitotic cells. Therefore, a constant supply of nucleotides is required...
Resource Type:: Dissertation
Full Text:: also like to thank Dr. Tory M. Hagen, Dr. Indira Rajagopal and Ms.Linda J. Wheeler for their advice

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Creator:: Young, James Patrick
Abstract:: The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Resource Type:: Dissertation

Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

Creator:: Zhang, Xiaolin
Resource Type:: Dissertation

Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics

Creator:: Thompson, Cynthia Sue
Abstract:: The effects of oligoribonucleotides of identical or similar sequences to a ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional...
Resource Type:: Dissertation

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Creator:: Purohit, Sarla
Abstract:: The dihydrofolate reductase gene of bacteriophate T4 has been investigated at the structural level to elucide the unusual regulation of expression of this gene and possible evolutionary relationships between the ohage and drug resistance factors-encoded dihydrofolate reductases (DHFR). A 1.1-kilobase-pair restriction fragment of the T4 multiple mutant dec8 containing frd,...
Resource Type:: Dissertation
Full Text:: Reductase 13 I. Primary Structure of Dihydro- folate Reductases 17 J. Importance and Interest in the Study

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Creator:: Martomo, Stella A.
Abstract:: Deoxyribonucleoside triphosphate (dNTP) concentrations measured in cells are not symmetric. dGTP almost always represents only 5-10% of the total dNTP pools in cells. In an in vitro replication system involving semiconservative replication from an SV 40 origin, the mutation frequency of an M13 phagemid replicated by human cell extracts in...
Resource Type:: Dissertation
Full Text:: , Dr. Tory Hagen and Dr. Soichi Kimura; also Dr. Isaac Wong. Dr. Jeff Greenwood, Dr. Sam Bradford

The effects of protein associations on pyrimidine deoxyribonucleotide biosynthesis

Creator:: McGaughey, Kathleen M.
Abstract:: The faithful replication of DNA depends on the appropriate balance of DNA precursors. From studies conducted in bacteriophage T4, models for deoxyribonucleotide biosynthesis producing pools appropriate for DNA replication have made it possible to understand more complex systems. A portion of that body of evidence supports the concept that deoxyribonucleotide...
Resource Type:: Dissertation
Full Text:: .- DNA NA Figure Ii. / \ 4Z-4 r tL #.,_w. '.tA ,.J / . ,.,.,.L_.. _,_Ic__fl II NA NA NANA. 8 S-NA

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Creator:: Bestwick, Richard Keith
Abstract:: This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
Resource Type:: Dissertation
Full Text:: , 1982 Typed by Priscilla J. Burk for Richard K. Bestwick Acknowledgements I wish to thank first and

Host kinases involved in DNA precursor biosynthesis during bacteriophage T4 infection

Creator:: Bernard, Mark Aguirre
Abstract:: Although the Escherichia coli host has almost all of the enzymes necessary to synthesize nucleotides needed for bacteriophage T4 DNA replication, phage genes expressed early in infection encode enzymes for de novo DNA precursor biosynthesis and salvage from degraded host DNA. Eight early enzymes and two host enzymes comprise the...
Resource Type:: Dissertation

ScholarsArchive@OSU

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

The effects of protein associations on pyrimidine deoxyribonucleotide biosynthesis

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Host kinases involved in DNA precursor biosynthesis during bacteriophage T4 infection

Limit your search

Academic Affiliation

Advisor

Commencement Year

Committee Member

Creator

Date

Decade

Degree Field

Degree Level

Degree Name

Language

License

Non-Academic Affiliation

Peer Reviewed

Resource Type

Rights Statement

Subject

Search Constraints

Search Results

Limit your search