Index Catalog // ScholarsArchive@OSU

Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

Creator:: Sargent, Roy Geoffrey
Abstract:: Imbalanced deoxyribonucleoside triphosphate (dNTP) pools are mutagenic for DNA synthesis in both intact cells and cell-free replication systems. Almost certainly, such mutagenesis involves competition between correctly and incorrectly base-paired precursors at replication sites. However, there are certain differences between the intact cell and cell-free systems that do not always allow...
Resource Type:: Dissertation
Full Text:: Deoxyribonucleotides as determinants of DNA replication

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Creator:: Davis, Ralph Eugene, 1957-
Abstract:: Vaccinia virus infected monkey kidney cells had been previously shown to have an increased ribonucleoside diphosphate reductase (RR) activity. DNA from mutant virus resistant to hydroxyurea were digested with restriction endonucleases and were shown to have substoichiometric amounts of the Hind III F fragment. Additional information from Southern blotting experiments...
Resource Type:: Dissertation
Full Text:: there has been no reported splicing of vaccinia transcripts. The early RNA is extruded from the viral

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Creator:: Purohit, Sarla
Abstract:: The dihydrofolate reductase gene of bacteriophate T4 has been investigated at the structural level to elucide the unusual regulation of expression of this gene and possible evolutionary relationships between the ohage and drug resistance factors-encoded dihydrofolate reductases (DHFR). A 1.1-kilobase-pair restriction fragment of the T4 multiple mutant dec8 containing frd,...
Resource Type:: Dissertation
Full Text:: least five sequences which show homology to the consensus sequence of E. coli promoters; however, no

Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics

Creator:: Thompson, Cynthia Sue
Abstract:: The effects of oligoribonucleotides of identical or similar sequences to a ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional...
Resource Type:: Dissertation
Full Text:: experiments were performed in the presence and absence of the inducer isopropylthiogalactoside (lPTG). No

Mutagenic mechanisms associated with perturbations of DNA precursor biosynthesis in phage T4

Creator:: Ji, Jiuping
Abstract:: A crucial factor in determining the accuracy of DNA replication is maintenance of a balanced supply of deoxyribonucleoside triphosphates (dNTPs) at replication forks. Perturbation of dNTP biosynthesis can induce dNTP pool imbalance with deleterious genetic consequences, including increased mutagenesis, recombination, chromosomal abnormalities and cell death. Using the T4 bacteriophage system,...
Resource Type:: Dissertation
Full Text:: molecule, the genetic map is circular due to circular permutation of base sequences in the genome (Mathews

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Creator:: Young, James Patrick
Abstract:: The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Resource Type:: Dissertation
Full Text:: adolesence has brought a wealth of information about the biochemistry involved in bacteriophage T4

Host kinases involved in DNA precursor biosynthesis during bacteriophage T4 infection

Creator:: Bernard, Mark Aguirre
Abstract:: Although the Escherichia coli host has almost all of the enzymes necessary to synthesize nucleotides needed for bacteriophage T4 DNA replication, phage genes expressed early in infection encode enzymes for de novo DNA precursor biosynthesis and salvage from degraded host DNA. Eight early enzymes and two host enzymes comprise the...
Resource Type:: Dissertation
Full Text:: phosphorusnitrogen (P-N) bond at No of histidine-117. Enzymes other than Ndk have been shown to catalyze nucleoside

Bacteriophage T4 ribonucleotide reductase : genes and proteins

Creator:: Hanson, Eric Scott
Resource Type:: Dissertation
Full Text:: of CDP could be reduced by 1 mole of RNR when no external reductant was present (Thelander, 1974

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Creator:: Bestwick, Richard Keith
Abstract:: This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
Resource Type:: Dissertation
Full Text:: economy of genetic information. There are no introns in any genes, very few if any non-coding bases

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

Creator:: Song, Shiwei
Abstract:: It is well known that the mitochondrial genome has a much higher spontaneous mutation rate than the nuclear genome. mtDNA mutations have been identified in association with many diseases and aging. mtDNA replication continues throughout the cell cycle, even in post-mitotic cells. Therefore, a constant supply of nucleotides is required...
Resource Type:: Dissertation
Full Text:: pathways. However, to date, there is no information available about the relative contribution of each

ScholarsArchive@OSU

Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics

Mutagenic mechanisms associated with perturbations of DNA precursor biosynthesis in phage T4

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Host kinases involved in DNA precursor biosynthesis during bacteriophage T4 infection

Bacteriophage T4 ribonucleotide reductase : genes and proteins

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

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