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Alternatives to the DNA precursor-synthesizing enzyme complex hypothesis

Creator:: Weitz, Stephen L.
Abstract:: Investigators have been studying an aggregate containing enzymes of deoxyribonucleoside triphosphate biosynthesis in T4-phage infected bacteria. They suggest that it behaves as an organized complex, efficiently channeling DNA precursors to the replication apparatus. Previous work has suggested that about 10 enzyme activities remain associated through several fractionation steps. This multi-enzyme...
Resource Type:: Dissertation
Full Text:: reported to sediment through sucrose gradients at 15-20 S. Also, individual activities are kinetically

Protein-protein interactions in the bacteriophage T4 dNTP synthetase complex

Creator:: Shen, Rongkun
Abstract:: The enzymes of dNTP synthesis in T4 infection associate to form a multienzyme complex, the T4 dNTP synthetase complex, facilitating the flow of metabolites en route to dNTPs, and their subsequent flow into DNA. Study of protein-protein interactions helps one to understand how the enzymes in the complex are organized...
Resource Type:: Dissertation
Full Text:: mature progeny. Figures 1.1 and 1.2 show the major events of phage T4’s life cycle. Unlike λ phage, T4

Host kinases involved in DNA precursor biosynthesis during bacteriophage T4 infection

Creator:: Bernard, Mark Aguirre
Abstract:: Although the Escherichia coli host has almost all of the enzymes necessary to synthesize nucleotides needed for bacteriophage T4 DNA replication, phage genes expressed early in infection encode enzymes for de novo DNA precursor biosynthesis and salvage from degraded host DNA. Eight early enzymes and two host enzymes comprise the...
Resource Type:: Dissertation
Full Text:: metabolic pathway. 4 Nucleotide Metabolism during Bacteriophage T4 Infection host cell DNA ADP luau s

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Creator:: Wang, Yeong
Abstract:: Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an enzyme unique to the deoxyribonucleotide metabolism of T-even bacteriophages. To study biochemical and biophysical properties of the enzyme, as well as the interaction of dCMP hydroxymethylase with other DNA precursor biosynthetic enzymes in vitro, availability of large amounts of the enzyme is...
Resource Type:: Dissertation
Full Text:: DP I hm-dCTP 1 POlym 00000 N D P Pone S. Wass ISNIAP dGDP dGT P Figure I-1. Reactions of DNA

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Creator:: Purohit, Sarla
Abstract:: The dihydrofolate reductase gene of bacteriophate T4 has been investigated at the structural level to elucide the unusual regulation of expression of this gene and possible evolutionary relationships between the ohage and drug resistance factors-encoded dihydrofolate reductases (DHFR). A 1.1-kilobase-pair restriction fragment of the T4 multiple mutant dec8 containing frd,...
Resource Type:: Dissertation
Full Text:: Overproduction of DHFR 59 R. Preparation of Crude Extract 59 S. Fractionation of E. coli and T4 DHFRs 60 T

Deoxyguanosine triphosphate, a possible target for reactive oxygen species-induced mutagenesis

Creator:: Tassotto, Mary Lynn Benka
Abstract:: Intracellular dNTP pool sizes are highly asymmetric, with dGTP usually comprising 5 to 10% of the sum of the dNTP pools. The work presented in this dissertation addresses the question of whether the underrepresentation of dGTP is related to its potential to be oxidized by reactive oxygen species. 8-oxo-guanine is...
Resource Type:: Dissertation
Full Text:: ................................................................ 83 5.2.5. Controls for the HPLC-ECD Assay of 8-oxo-dGTP Pools ............ 84 5.2.6. EC Detection of

Bacteriophage T4 ribonucleotide reductase : genes and proteins

Creator:: Hanson, Eric Scott
Resource Type:: Dissertation
Full Text:: Commencement June 1995 Doctor of Philosophy thesis of Eric S. Hanson presented on September 9, 1994

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Creator:: Young, James Patrick
Abstract:: The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Resource Type:: Dissertation
Full Text:: Synthase III-7. Elution of Phage Thymidylate Synthase From a S-Sepharose 71 Cation Exchange Column and an

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Creator:: Bestwick, Richard Keith
Abstract:: This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
Resource Type:: Dissertation
Full Text:: that replication occurs during a six to eight hour period, the S-phase, representing one-third to one

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

Creator:: Spiro, Craig
Abstract:: Vaccinia virus-infected animal cells have been used to study the interactions between the replication of deoxyribonucleic acid (DNA) and the biosynthesis of its nucleotide precursors. Some antimetabolites that inhibit DNA replication have as their targets enzymes of nucleotide biosynthesis. Furthermore, the disruption of nucleotide metabolism can alter the fidelity of...
Resource Type:: Dissertation
Full Text:: DNA precursor enzymes in eukaryotic cells. They report that, during S phase, enzymes of DNA

ScholarsArchive@OSU

Alternatives to the DNA precursor-synthesizing enzyme complex hypothesis

Protein-protein interactions in the bacteriophage T4 dNTP synthetase complex

Host kinases involved in DNA precursor biosynthesis during bacteriophage T4 infection

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Deoxyguanosine triphosphate, a possible target for reactive oxygen species-induced mutagenesis

Bacteriophage T4 ribonucleotide reductase : genes and proteins

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

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