Index Catalog // ScholarsArchive@OSU

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

Creator:: Song, Shiwei
Abstract:: It is well known that the mitochondrial genome has a much higher spontaneous mutation rate than the nuclear genome. mtDNA mutations have been identified in association with many diseases and aging. mtDNA replication continues throughout the cell cycle, even in post-mitotic cells. Therefore, a constant supply of nucleotides is required...
Resource Type:: Dissertation
Full Text:: ) Am JHum Genet. 64, 1276-1281. Bridges, B. G., Jiang, Z., and Cheng, YC. (1999) 1 Biol. Chem. 274

Regulation of ribonucleotide reductase analyzed by simultaneous measurement of the four enzyme activities

Creator:: Hendricks, Stephen P.
Abstract:: The first committed step in DNA biosynthesis occurs by direct reduction of ribonucleotides. This reduction is catalyzed by ribonucleotide reductase (RNR), an enzyme which uses a unique radical mechanism to facilitate the transformation. All four DNA precursors are synthesized by a single enzyme. Therefore, an intricate pattern of regulation has...
Resource Type:: Dissertation
Full Text:: Cheng, 1979; Cory et al., 1985) and presented in Chapter 4 of this thesis, suggests that allosteric

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

Creator:: Spiro, Craig
Abstract:: Vaccinia virus-infected animal cells have been used to study the interactions between the replication of deoxyribonucleic acid (DNA) and the biosynthesis of its nucleotide precursors. Some antimetabolites that inhibit DNA replication have as their targets enzymes of nucleotide biosynthesis. Furthermore, the disruption of nucleotide metabolism can alter the fidelity of...
Resource Type:: Dissertation
Full Text:: in cytotoxicity (Major et al., 1982; Cheng and Nakayama, 1983). The relative importance of these

Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

Creator:: Zhang, Xiaolin
Resource Type:: Dissertation
Full Text:: depends entirely on the neighboring sequence (Cheng et al., 1992). Goodman and his collaborators

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Creator:: Bestwick, Richard Keith
Abstract:: This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
Resource Type:: Dissertation

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Creator:: Davis, Ralph Eugene, 1957-
Abstract:: Vaccinia virus infected monkey kidney cells had been previously shown to have an increased ribonucleoside diphosphate reductase (RR) activity. DNA from mutant virus resistant to hydroxyurea were digested with restriction endonucleases and were shown to have substoichiometric amounts of the Hind III F fragment. Additional information from Southern blotting experiments...
Resource Type:: Dissertation
Full Text:: inhibiting tk while dTMP is stimulatory (Cheng, 1978). This enzyme is also able to use deoxyuridine and

Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

Creator:: Sargent, Roy Geoffrey
Abstract:: Imbalanced deoxyribonucleoside triphosphate (dNTP) pools are mutagenic for DNA synthesis in both intact cells and cell-free replication systems. Almost certainly, such mutagenesis involves competition between correctly and incorrectly base-paired precursors at replication sites. However, there are certain differences between the intact cell and cell-free systems that do not always allow...
Resource Type:: Dissertation
Full Text:: eucaryotic and procaryotic cells investigated (Thelander and Reichard, 1979; Nutter and Cheng, 1984) as

DNA precursor compartmentation in mammalian cells : distribution and rates of equilibration between nucleus and cytoplasm

Creator:: Leeds, Janet M.
Abstract:: A rapid nuclear isolation technique was adapted in order to examine the question of DNA precursor compartmentation in mammalian cells. By using this method a reproducible proportion of the cellular nucleotides remained associated with the isolated nuclei. Examination, at several different cell densities, of exponentially growing HeLa cells showed that...
Resource Type:: Dissertation
Full Text:: and sonicated. Our measurements (Fig. 2.7) were similar to results reported by others (Cheng et al

Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

Creator:: Mun, Byung-joo
Abstract:: Although the synthesis of DNA precursors is closely coordinated with DNA replication, it is still not clear whether deoxyribonucleoside triphosphates (dNTPs) influence DNA replication independently of their interactions with DNA polymerase catalytic sites. In an effort to understand the extent to which rate and fidelity of DNA replication are regulated...
Resource Type:: Dissertation
Full Text:: regulated by their respective dNTPs (Cheng, 1978; Bohman and Eriksson, 1988), while the activities of

Deoxyguanosine triphosphate, a possible target for reactive oxygen species-induced mutagenesis

Creator:: Tassotto, Mary Lynn Benka
Abstract:: Intracellular dNTP pool sizes are highly asymmetric, with dGTP usually comprising 5 to 10% of the sum of the dNTP pools. The work presented in this dissertation addresses the question of whether the underrepresentation of dGTP is related to its potential to be oxidized by reactive oxygen species. 8-oxo-guanine is...
Resource Type:: Dissertation

ScholarsArchive@OSU

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

Regulation of ribonucleotide reductase analyzed by simultaneous measurement of the four enzyme activities

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

DNA precursor compartmentation in mammalian cells : distribution and rates of equilibration between nucleus and cytoplasm

Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

Deoxyguanosine triphosphate, a possible target for reactive oxygen species-induced mutagenesis

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