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Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

Creator:: Zhang, Xiaolin
Resource Type:: Dissertation
Full Text:: Asymmetry. Abstract approved: Christopher K. Mathews ABSTRACT A major goal of genetics is to

Mutagenic mechanisms associated with perturbations of DNA precursor biosynthesis in phage T4

Creator:: Ji, Jiuping
Abstract:: A crucial factor in determining the accuracy of DNA replication is maintenance of a balanced supply of deoxyribonucleoside triphosphates (dNTPs) at replication forks. Perturbation of dNTP biosynthesis can induce dNTP pool imbalance with deleterious genetic consequences, including increased mutagenesis, recombination, chromosomal abnormalities and cell death. Using the T4 bacteriophage system,...
Resource Type:: Dissertation
Full Text:: Perturbations of DNA Precursor Biosynthesis in Phage T4 Redacted for Privacy Abstract Approved: Christopher K

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

Creator:: Spiro, Craig
Abstract:: Vaccinia virus-infected animal cells have been used to study the interactions between the replication of deoxyribonucleic acid (DNA) and the biosynthesis of its nucleotide precursors. Some antimetabolites that inhibit DNA replication have as their targets enzymes of nucleotide biosynthesis. Furthermore, the disruption of nucleotide metabolism can alter the fidelity of...
Resource Type:: Dissertation
Full Text:: Metabolism in Vaccinia Virus-Infected Mammalian Cells. Abstract Redacted for Privacyapproved: Christopher K

Attempted cloning of T4 phage ribonucleotide reductase

Creator:: Booth, Joseph
Abstract:: Ribonucleotide reductase is an important enzyme in the control of DNA replication within the cell. Ribonucleotide reductase exerts its control through enzymatic reduction of nucleoside diphosphates. The bacteriophage T4 enzyme is an example of the class of iron-requiring reductases which also includes E. coli and mammalian ribonucleotide reductases. The two...
Resource Type:: Masters Thesis
Full Text:: model exist, however (Ator, M. A. and Stubbe, J., 1985; Spector, T. and Jones, T. E., 1985). F. RDPR

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Creator:: Martomo, Stella A.
Abstract:: Deoxyribonucleoside triphosphate (dNTP) concentrations measured in cells are not symmetric. dGTP almost always represents only 5-10% of the total dNTP pools in cells. In an in vitro replication system involving semiconservative replication from an SV 40 origin, the mutation frequency of an M13 phagemid replicated by human cell extracts in...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Christopher K. Mathews Deoxyribonucleoside triphosphate (dNTP) concentrations measured

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Creator:: Wang, Yeong
Abstract:: Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an enzyme unique to the deoxyribonucleotide metabolism of T-even bacteriophages. To study biochemical and biophysical properties of the enzyme, as well as the interaction of dCMP hydroxymethylase with other DNA precursor biosynthetic enzymes in vitro, availability of large amounts of the enzyme is...
Resource Type:: Dissertation
Full Text:: Acknowledgement I wish to express my sincere thanks to my major advisor, Dr. Christopher K Mathews, for his

Large subunit of vaccinia cirus ribonucleotide reductase : affinity chromatography-based purification and photoaffinity labeling

Creator:: Warth, Rainer K.
Abstract:: Ribonucleoside diphosphate reductase (RR) from vaccinia virus was recently cloned and overexpressed rn Escherichia coli. The amino acid sequence identities of the small and large subunits between the mouse and the vaccinia virus reductase are approximately 80 and 72 percent, respectively. In addition, vaccinia virus RR displays similar complex allosteric...
Resource Type:: Masters Thesis
Full Text:: Purification and Photoaffinity Labeling Abstract approved: Christopher K. Mathews, Ph.D. Ribonucleoside

Protein-protein interactions in the bacteriophage T4-coded dCTPase-dUTPase

Creator:: Ungermann, Christian
Resource Type:: Masters Thesis
Full Text:: like to thank Dr. Christopher K. Mathews for the opportunity to work in his laboratory and for his

Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

Creator:: Mun, Byung-joo
Abstract:: Although the synthesis of DNA precursors is closely coordinated with DNA replication, it is still not clear whether deoxyribonucleoside triphosphates (dNTPs) influence DNA replication independently of their interactions with DNA polymerase catalytic sites. In an effort to understand the extent to which rate and fidelity of DNA replication are regulated...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Redacted for Privacy Christopher K. Mathews Although the synthesis of DNA precursors

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Creator:: Bestwick, Richard Keith
Abstract:: This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
Resource Type:: Dissertation
Full Text:: : Christopher K. Mathews This dissertation describes an investigation of DNA precursor supply for

ScholarsArchive@OSU

Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

Mutagenic mechanisms associated with perturbations of DNA precursor biosynthesis in phage T4

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

Attempted cloning of T4 phage ribonucleotide reductase

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Large subunit of vaccinia cirus ribonucleotide reductase : affinity chromatography-based purification and photoaffinity labeling

Protein-protein interactions in the bacteriophage T4-coded dCTPase-dUTPase

Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

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