Index Catalog // ScholarsArchive@OSU

Large subunit of vaccinia cirus ribonucleotide reductase : affinity chromatography-based purification and photoaffinity labeling

Creator:: Warth, Rainer K.
Abstract:: Ribonucleoside diphosphate reductase (RR) from vaccinia virus was recently cloned and overexpressed rn Escherichia coli. The amino acid sequence identities of the small and large subunits between the mouse and the vaccinia virus reductase are approximately 80 and 72 percent, respectively. In addition, vaccinia virus RR displays similar complex allosteric...
Resource Type:: Masters Thesis
Full Text:: Rainer K. Warth for the degree of Master of Science in Biochemistry and Biophysics presented on August 31

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Creator:: Young, James Patrick
Abstract:: The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Resource Type:: Dissertation
Full Text:: Christopher K. Mathews, Ph.D. The ability to faithfully replicate DNA is dependent upon the maintenance and

Protein-protein interactions in the bacteriophage T4 dNTP synthetase complex

Creator:: Shen, Rongkun
Abstract:: The enzymes of dNTP synthesis in T4 infection associate to form a multienzyme complex, the T4 dNTP synthetase complex, facilitating the flow of metabolites en route to dNTPs, and their subsequent flow into DNA. Study of protein-protein interactions helps one to understand how the enzymes in the complex are organized...
Resource Type:: Dissertation
Full Text:: Synthetase Complex Abstract approved: Christopher K. Mathews The

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

Creator:: Song, Shiwei
Abstract:: It is well known that the mitochondrial genome has a much higher spontaneous mutation rate than the nuclear genome. mtDNA mutations have been identified in association with many diseases and aging. mtDNA replication continues throughout the cell cycle, even in post-mitotic cells. Therefore, a constant supply of nucleotides is required...
Resource Type:: Dissertation
Full Text:: Diseases Abstract approved:_ ___ Christopher K. Mathews It is well known that the mitochondrial genome

Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

Creator:: Zhang, Xiaolin
Resource Type:: Dissertation
Full Text:: Asymmetry. Abstract approved: Christopher K. Mathews ABSTRACT A major goal of genetics is to

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Creator:: Wang, Yeong
Abstract:: Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an enzyme unique to the deoxyribonucleotide metabolism of T-even bacteriophages. To study biochemical and biophysical properties of the enzyme, as well as the interaction of dCMP hydroxymethylase with other DNA precursor biosynthetic enzymes in vitro, availability of large amounts of the enzyme is...
Resource Type:: Dissertation
Full Text:: Acknowledgement I wish to express my sincere thanks to my major advisor, Dr. Christopher K Mathews, for his

Organization of the T4 dNTP synthetase complex at DNA replication sites

Creator:: Kim, JuHyun
Abstract:: With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
Resource Type:: Dissertation
Full Text:: of the T4 dNTP Synthetase Complex at DNA Replication Sites Abstract approved: Christopher K

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Creator:: Purohit, Sarla
Abstract:: The dihydrofolate reductase gene of bacteriophate T4 has been investigated at the structural level to elucide the unusual regulation of expression of this gene and possible evolutionary relationships between the ohage and drug resistance factors-encoded dihydrofolate reductases (DHFR). A 1.1-kilobase-pair restriction fragment of the T4 multiple mutant dec8 containing frd,...
Resource Type:: Dissertation
Full Text:: I thank Dr. Christopher K. Mathews for his guidance and support. His patience, stimulating

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Creator:: Martomo, Stella A.
Abstract:: Deoxyribonucleoside triphosphate (dNTP) concentrations measured in cells are not symmetric. dGTP almost always represents only 5-10% of the total dNTP pools in cells. In an in vitro replication system involving semiconservative replication from an SV 40 origin, the mutation frequency of an M13 phagemid replicated by human cell extracts in...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Christopher K. Mathews Deoxyribonucleoside triphosphate (dNTP) concentrations measured

Attempted cloning of T4 phage ribonucleotide reductase

Creator:: Booth, Joseph
Abstract:: Ribonucleotide reductase is an important enzyme in the control of DNA replication within the cell. Ribonucleotide reductase exerts its control through enzymatic reduction of nucleoside diphosphates. The bacteriophage T4 enzyme is an example of the class of iron-requiring reductases which also includes E. coli and mammalian ribonucleotide reductases. The two...
Resource Type:: Masters Thesis
Full Text:: bacteriophage, (2) the herpesvirus, and (3) vaccinia virus (Slabaugh, M. B., Mathews, C. K., 1984). Much

ScholarsArchive@OSU

Large subunit of vaccinia cirus ribonucleotide reductase : affinity chromatography-based purification and photoaffinity labeling

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Protein-protein interactions in the bacteriophage T4 dNTP synthetase complex

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Organization of the T4 dNTP synthetase complex at DNA replication sites

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Attempted cloning of T4 phage ribonucleotide reductase

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