Nisin is an antibacterial peptide, which when adsorbed
on a surface can inhibit bacterial adhesion and viability.
The ability of noncovalently immobilized nisin to withstand
exchange by the milk proteins bovine serum albumin, β-lactoglobulin, α-lactalbumin, and β-casein on surfaces that
had been silanized with dichlorodiethylsilane to exhibit
high and low...
β-lactoglobulin (β-Lg), bovine serum albumin (BSA), α-lactalbumin
(α-Lac), and β-casein were adsorbed onto
silanized silica surfaces of low and high hydrophobicity
for 8 h, and β-Lg and BSA for 1 h. The surfaces were
incubated in buffer for 0, 5, 10, or 15 h and then contacted
with Listeria monocytogenes...
The adsorption kinetics exhibited by selected charge mutants of T4 lysozyme at
silanized silica surfaces were monitored with in situ ellipsometry. Mutant lysozymes were
produced by substitution of lysine (Lys) with glutamic acid (Glu). Each substitution
resulted in a decrease in the net charge of the protein by 2 units....