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Filtering by: Creator Loughborough, W. Karl Remove constraint Creator: Loughborough, W. Karl Advisor Mcguire, Joseph Remove constraint Advisor: Mcguire, Joseph License All rights reserved Remove constraint License: All rights reserved

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  • Binding of bacterial lipopolysaccharide by the cationic amphiphilic peptide WLBU2 at interfaces

    Creator:
    Ryder, Matthew P.
    Abstract:
    Passage of blood through a sorbent device for removal of bacteria and endotoxin by specific binding with immobilized, membrane-active, bactericidal peptides holds promise for treating severe blood infections. Peptide insertion in the target membrane and stable binding is desirable, while membrane disruption and release of degradation products to the circulating...
    Resource Type:
    Dissertation
    Full Text:
    Karl F. Schilke_ Passage of blood through a sorbent device for removal of bacteria and endotoxin by

  • Molecular origins of peptide entrapment in polyethylene oxide layers

    Creator:
    Wu, Xiangming
    Abstract:
    A more quantitative understanding of peptide entrapment and elution from otherwise protein-repellent polyethylene oxide (PEO) brush layers will provide direction for development of new strategies for drug storage and delivery. Here we describe criteria for peptide integration and structural change within the PEO brush, and discuss the reversibility of peptide...
    Resource Type:
    Dissertation
    Full Text:
    : _____________________________________________________________________ Joseph McGuire Karl F. Schilke A more quantitative understanding of

  • Quantification of fibrinogen adsorption to nisin-loaded polyethylene oxide layers

    Creator:
    Auxier, Julie A.
    Abstract:
    Antimicrobial coatings used to reduce the risk of infection caused by tissue-contacting medical devices must address specific biocompatibility requirements including prevention of thrombosis and rise of bacterial resistance. An antimicrobial-loaded poly(ethylene-oxide) (PEO) coating has been tentatively shown to be non-fouling with sustained antimicrobial activity. However, the presence of the antimicrobial,...
    Resource Type:
    Honors College Thesis
    Full Text:
    also in my overall professional development. I also extend my gratification to Dr. Karl Schilke for

  • Synthesis and evaluation of PEO-coated materials for microchannel-based hemodialysis

    Creator:
    Heintz, Keely
    Abstract:
    The marked increase in surface-to-volume ratio associated with microscale devices for hemodialysis leads to problems with hemocompatibility and blood flow distribution that are more challenging to manage than those encountered at the conventional scale. In this work, stable surface modifications with pendant polyethylene oxide (PEO) chains were produced on polycarbonate...
    Resource Type:
    Masters Thesis
    Full Text:
    learning experience! Thanks to my second-in-line (but not least!) advisor Dr. Karl “Rat” Schilke for

  • Retention of protein repulsive character and antimicrobial activity of PEO brush layers following nisin entrapment

    Creator:
    Auxier, Julie A.
    Abstract:
    Nisin, an amphiphilic, antimicrobial peptide, has been shown to integrate into the hydrophobic inner region of poly(ethylene oxide) (PEO) brush layers; however, the presence of integrated nisin may compromise the protein repulsive character of the PEO layer. In particular, the introduction of fibrinogen to nisin-loaded brush layers has been observed...
    Resource Type:
    Masters Thesis
    Full Text:
    all his guidance and support. I also extend my sincere gratitude to Dr. Karl Schilke, who I will

  • Quantifying nisin adsorption behavior at pendant polyethylene oxide brush layers

    Creator:
    Dill, Justen K.
    Abstract:
    A more quantitative understanding of peptide loading and release from polyethylene oxide (PEO) brush layers will provide direction for development of new strategies for drug storage and delivery. The antimicrobial peptide nisin shows potent activity against Gram-positive bacteria including the most prevalent implant-associated pathogens, its mechanism of action minimizes the...
    Resource Type:
    Masters Thesis
    Full Text:
    be learned in a laboratory. My sincere appreciation goes out to Dr. Karl Schilke who was absolutely

  • Characterization and application of PEO-containing triblock copolymer surfactants

    Creator:
    Snider, Joshua L.
    Abstract:
    The action of pendant, polyethylene oxide (PEO) brush layers as nonfouling coatings for biomedical materials is well understood. However progress toward clinical application of stable, high density PEO coatings has historically been impeded by the lack of cost effective, noninvasive methods for their preparation. Triblock polymers featuring a hydrophobic, polypropylene...
    Resource Type:
    Honors College Thesis
    Full Text:
    , Dr. Joseph McGuire, Dr. Karl Schilke, and Dr. Phil Harding for all of their valuable guidance

  • Functionalization of Surfaces with Nisin in a Polyethylene Oxide Brush Layer

    Creator:
    Ryder, Matthew P.
    Abstract:
    Infections in hospitals account for over 100,000 deaths per year. These infections occur at the hospital from complications following bacterial adhesion to intravenous catheters, coronary stents and other implanted devices. Another common problem is protein adsorption to the surface of the device and subsequent blood clotting. Methods for combating these...
    Resource Type:
    Honors College Thesis
    Full Text:
    .            Matthew P Ryder, Author      Acknowledgements    I would like to thank Karl Schilke and Jeff Tai for

  • Molecular Origins of Recombinant Factor VIII (rFVIII)

    Creator:
    Tadehara, Katherine M.
    Abstract:
    The capability of Pluronic® F68 to stabilize human recombinant Factor VIII in solution was assessed using zeta potential measurements. Hemophilia A is a genetic disorder in which the body is missing Factor VIII (FVIII) from the blood coagulation cascade. The pharmaceutical industry developed recombinant Factor VIII (rFVIII), the largest cloned...
    Resource Type:
    Honors College Thesis
    Full Text:
    we needed it especially when we started second guessing our math. Thank you, Dr. Karl “Rat” Schilke

  • Nisin adsorption to PEO-PPO-PEO tri-block copolymer layers and its resistance to elution by fibrinogen

    Creator:
    Ryder, Matthew P.
    Abstract:
    While hydrophobic surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] (PEO-PPO-PEO) surfactant Pluronic® F108 are highly resistant to plasma protein adsorption, the antimicrobial peptide nisin has been observed to adsorb in multilayer quantities at such surfaces, and the PEO chains themselves suggested to inhibit nisin exchange by blood proteins. But...
    Resource Type:
    Masters Thesis
    Full Text:
    may never have found my passion. I would also like to say thank you to Karl "Rat" Schilke, who was