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Filtering by: Creator Machovina, Melodie M. Advisor Mehl, Ryan A.

Engineering bioorthogonal reactions on proteins in live cells

Creator:

Machovina, Melodie M. and Mehl, Ryan A.

Abstract:

This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...

Resource Type:

Poster
Engineering bioorthogonal reactions on proteins in live cells

Creator:

Machovina, Melodie M. and Mehl, Ryan A.

Abstract:

This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...

Resource Type:

Poster
Exploring the Limits of Ligation Rate and Specificity in Protein Immobilization Using the Genetically Encoded Tetrazine Ligation

Creator:

Bednar, Riley M.

Abstract:

Immobilized proteins are integral components of a number of important technologies, including the principal component of many biosensors, as functional coatings in various implants, as catalysts is industrial biosynthesis, and as the building blocks of next-generation nanotechnologies. Despite their ubiquity, relatively little is known about the processes that shape immobilized...

Resource Type:

Dissertation

Full Text:

AN ABSTRACT OF THE DISSERTATION OF Riley M