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Filtering by: Creator Paraguas, Ferdinand J. Remove constraint Creator: Paraguas, Ferdinand J. Advisor Montgomery, Morris W. Remove constraint Advisor: Montgomery, Morris W. Degree Name Doctor of Philosophy (Ph.D.) Remove constraint Degree Name: Doctor of Philosophy (Ph.D.)

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  • Purification and properties of d'Anjou pear (Pyrus communis, L.) polyphenol oxidase

    Creator:
    Wissemann, Kimberly Warner
    Abstract:
    Polyphenol oxidase (PPO) from d'Anjou pears was purified and some molecular properties were studied. The extraction of PPO was accomplished in the presence of the phenolic binder AG 2X-8 and Triton X-100. Chlorophyll pigment was removed by chromatography resulting in a clear, colorless enzyme extract. Three fractions of pear PPO...
    Resource Type:
    Dissertation
    Full Text:
    homogeneity are from potato (Patil and Zucker, 1965; Balasingham and Ferdinand, 1970), Bartlett pear

  • Inhibition of polyphenol oxidase by sulfur dioxide

    Creator:
    Sayavedra-Soto, Luis Alberto
    Abstract:
    Inhibition of polyphenol oxidase (PPO) by sulfur dioxide (SO₂) was studied using three different sources of PPO (banana, mushroom, and pear). Several methods to detect PPO activity were tested due to SO₂, interference in some of the assays. The method using 2-nitro-5-thiobenzoic acid (TNB) to react with the quinones was...
    Resource Type:
    Dissertation
    Full Text:
    Department of Food Science and Technology J Dean of Graduate'pchool Date thesis is presented October 3

  • Purification of strawberry polyphenol oxidase and its role in anthocyanin degradation

    Creator:
    Wesche-Ebeling, Pedro Alfredo E.
    Abstract:
    Polyphenol oxidase (PPO) from strawberries was purified, partially characterized, and used to study its role in the degradation of anthocyanin pigments. The extraction method included the use of a buffered solution containing the phenolic binders Polyclar AT and Amberlite XAD-4. Addition of Triton X-100 resulted in an increase of the...
    Resource Type:
    Dissertation
    Full Text:
    Ferdinand, 1970) and, pear PPO (Wisseman, 1983). The Neurospora PPO structure has been studied in great