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Filtering by: Subject Adenoviruses Remove constraint Subject: Adenoviruses Advisor Pearson, George D. Remove constraint Advisor: Pearson, George D.

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  • The kinetics of adenovirus DNA replication

    Creator:
    Bodnar, John W.
    Abstract:
    Methods have been developed to measure the time required to complete replication of an adenovirus DNA molecule and the time between rounds of replication of the viral genomes. These methods show that 22 minutes are required to complete replication of an adenovirus DNA molecule at 37° C and that this...
    Resource Type:
    Dissertation

  • Intramolecular and intermolecular strand hybridization during adenovirus DNA replication

    Creator:
    Xu, Fu-yun
    Abstract:
    Adenovirus initiates strand-displacement DNA replication from origins located in identical inverted terminal repetitions (ITRs). Panhandle structures, formed by base-pairing between ITRs on the displaced strands, have been proposed as replication intermediates for complementary strand synthesis. Plasmid molecules containing a single adenovirus terminal sequence and specially arranged inverted repeat sequences were...
    Resource Type:
    Dissertation

  • In vitro adenovirus DNA replication

    Creator:
    Chow, Kuan-chih W.
    Resource Type:
    Dissertation

  • Adenovirus replication in trans : a new replication pathway

    Creator:
    Ahern, Kevin
    Abstract:
    Replication of plasmid molecules containing either a left-end or a right-end adenovirus origin was investigated. When the plasmids were linearized to expose their respective adenovirus termini, mixed, and transfected with helper adenovirus DNA into the human 293 cell line, a new molecule was detected. The new molecule was the size...
    Resource Type:
    Dissertation

  • Structural requirements for adenovirus DNA replication

    Creator:
    Hu, Chin-Hwa
    Abstract:
    The adenovirus replication origins reside in the inverted terminal repetition (ITR) sequences. Replication proceeds unidirectionally by a strand-displacement mechanism from either or both origins to produce duplex daughter DNA plus a displaced parental strand. The displaced strand can be duplicated by two different routes. The cis pathway involves intramolecular hybridization...
    Resource Type:
    Dissertation

  • Adenovirus complementary strand synthesis in vivo

    Creator:
    Wang, Kai, 1955-
    Abstract:
    Replication of plasmid molecules containing a single adenovirus terminal sequence and specially arranged inverted repeat sequences were studied. When the plasmids were linearized to expose the adenovirus terminal sequence and transfected into 293 cells with helper adenovirus DNA, a larger molecule was detected. The new molecule contained adenovirus terminal sequences...
    Resource Type:
    Dissertation

  • Sequence conversion during adenovirus DNA replication

    Creator:
    Bennett, Kelly L.
    Abstract:
    The work in this thesis has provided conclusive genetic evidence that "panhandle" intermediates form during adenovirus replication. Adenovirus chromosomes lacking 51 by from their left -hand termini are infectious and capable of regenerating the missing origin sequence. Yet if an entire inverted terminal repeat is removed, the adenovirus chromosome is...
    Resource Type:
    Dissertation