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Filtering by: Advisor Penner, Michael H. Committee Member Schimerlik, Michael Creator Piyachomkwan, Kuakoon

Interactions of Trichoderma reesei exo-acting cellulases with p-aminophenyl 1-thio-β-D-cellobioside

Creator:

Piyachomkwan, Kuakoon

Abstract:

Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases) enzymes. One approach to separating these enzymes is affinity chromatography. A new ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose. The property of APTC in affinity chromatography is demonstrated using Trichoderma...

Resource Type:

Dissertation