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Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

Creator:: Liaw, Ean-Tun
Abstract:: The influence of substrate and enzyme concentration on the rate of saccharification of two defined, insoluble, cellulose substrates, Avicel and Solka-Floc, by the cellulase enzyme system of Trichoderma viride has been evaluated. Assays utilized enzyme concentrations ranging from 0.014 to 0.056 filter paper unit per mL and substrate concentrations up...
Resource Type:: Masters Thesis
Full Text:: Complex from Trichoderma viride. Abstract approved: , . _ .. ... rr —.—^=— ^ ^ Michael H

Utilization of lignocellulosic polysaccharides

Creator:: Fenske, John J.
Abstract:: Lignocellulosic biomass represents a vast supply of fermentable carbohydrates and functional aromatic compounds. Conversion of lignocellulosics to ethanol and other useful products would be of widespread economical and environmental benefit. Better understanding of the behavior of different lignicellulosic feedstocks in fermentation protocols as well as catalytic activities involved in lignocellulosic...
Resource Type:: Dissertation
Full Text:: . John J. Fenske, Author Acknowledgements Professor Michael H. Penner has earned my deepest respect

Quantitative polysaccharide analysis of lignocellulosic biomass

Creator:: Fenske, John J.
Abstract:: Lignocellulosic biomass is a potential source of fermentable sugars such as glucose. Enzymatic hydrolysis of cellulose is a viable method of solubilizing the glucose from biomass, but the cellulose fraction of native lignocellulosic material is shielded from enzymatic attack by the lignin-hemicellulose matrix surrounding it. Pretreating lignocellulosic biomass with dilute...
Resource Type:: Masters Thesis
Full Text:: Polysaccharide Analysis of Lignocellulosic Biomass Abstract approved: ,. ^ , Michael H. Penner

Initial velocity analyses of milling effects on rates of enzyme-catalyzed saccharification of native wheat straw

Creator:: Nguyen, Quynh M.
Abstract:: There is considerable interest in developing environmentally friendly processes for the economical conversion of biomass plant material to biofuels, bioproducts and biomaterials. One approach to such a conversion process is to make use of enzymes for the conversion of plant polysaccharides to simple sugars; the simple sugars could then be...
Resource Type:: Masters Thesis
Full Text:: : _____________________________________________________ Michael H. Penner There is considerable interest in developing environmentally

The relative activity of the cellulase enzyme system of Trichoderma reesei with native and modified cellulosic substrates

Creator:: Liaw, Ean-Tun
Resource Type:: Dissertation
Full Text:: Substrates Abstract approved by : Michael H. Penner The relative merit of assays commonly applied to

Quantification and reactivity of cellulose reducing ends : implication for cellulose

Creator:: Kongruang, Sasithorn
Abstract:: The primary purpose of this study was to (1) develop methods for the analysis of and (2) provide information on the chemical nature of reducing ends in typical cellulose substrates used for the study of cellulolytic enzymes. The studies were designed such that values obtained for cellulose substrates were compared...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Michael H. Penn&John P. Bolte The primary purpose of this study was to (1) develop

Apparent inhibition of Pacific whiting surimi-associated protease by whey protein concentrate

Creator:: Piyachomkwan, Kuakoon
Abstract:: Surimi is a seafood product which is used to manufacture restructured products such as artificial crab and lobster. Surimi is produced from fish fillets by washing to remove sarcoplasmic proteins and increase the concentration of myofibrillar proteins, and mixing with cryoprotectants. A valuable attribute of surimi is its ability to...
Resource Type:: Masters Thesis
Full Text:: approved : . . . . Michael H. Penner, Ph. D. Surimi is a seafood product which is used to

Wheat lignin as a functional dietary fiber component

Creator:: Junyusen, Tiraporn
Abstract:: This dissertation has focused on lignin byproducts that result from the alkali processing of wheat straw. The first study evaluated the application of “standard” lignin quantification methods for summative analyses of alkali-based processes. Mass closure during NaOH processing at 25, 50, and 75°C of wheat straw was evaluated using the...
Resource Type:: Dissertation
Full Text:: . CONTRIBUTION OF AUTHORS Dr. Michael H. Penner and Dr. Lisbeth M. Goddik were involved with experimental

A filter paper assay for low cellulase activities and the cultivation of Trichoderma reesei on acid whey and sweet whey permeate

Creator:: Nordmark, Tor Soren
Abstract:: The traditional filter paper assay for saccharifying cellulase originally described by M. Mandels et al (1976) has been modified to make possible low activity determinations of Trichoderma cellulases. The enzymatic activity appears to decline during a prolonged incubation period if no precautions have been taken. By means of adding bovine...
Resource Type:: Masters Thesis
Full Text:: Acid Whev and Sweet Whev Permeate Abstract approved: Michael H. Penner The traditional filter

Porosity, surface area and enzymatic saccharification of microcrystalline cellulose

Creator:: Tantasucharit, Usicha
Abstract:: The research described in this thesis was aimed at understanding how particle size, porosity, and enzyme accessible surface area influence the rate of saccharification of microcrystalline cellulose. Microcrystalline cellulose (MCC) is a commonly used substrate for the study of cellulolytic enzymes. MCC preparations of different particle size are commercially available....
Resource Type:: Masters Thesis
Full Text:: yields [ES}=yV i L m J (2.10) By rearranging equation 2.10 Substituting equation 2.2 into

ScholarsArchive@OSU

Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

Utilization of lignocellulosic polysaccharides

Quantitative polysaccharide analysis of lignocellulosic biomass

Initial velocity analyses of milling effects on rates of enzyme-catalyzed saccharification of native wheat straw

The relative activity of the cellulase enzyme system of Trichoderma reesei with native and modified cellulosic substrates

Quantification and reactivity of cellulose reducing ends : implication for cellulose

Apparent inhibition of Pacific whiting surimi-associated protease by whey protein concentrate

Wheat lignin as a functional dietary fiber component

A filter paper assay for low cellulase activities and the cultivation of Trichoderma reesei on acid whey and sweet whey permeate

Porosity, surface area and enzymatic saccharification of microcrystalline cellulose

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