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Interactions of Trichoderma reesei exo-acting cellulases with p-aminophenyl 1-thio-β-D-cellobioside

Creator:: Piyachomkwan, Kuakoon
Abstract:: Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases) enzymes. One approach to separating these enzymes is affinity chromatography. A new ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose. The property of APTC in affinity chromatography is demonstrated using Trichoderma...
Resource Type:: Dissertation
Full Text:: : Interaction of Trichoderma reesei Exo- Acting Cellulases With Immobilized p-Aminophenyl 1-Thio-^-D

Immobilized cellooligosaccharides in the study of trichoderma reesei cellobiohydrolases

Creator:: Sangseethong, Kunruedee
Abstract:: A novel type of model substrates, i.e. immobilized p-aminophenyl-β-D-cellooligosaccharides, was developed and used in the study of exocellulases. The two major cellobiohydrolases from Trichoderma reesei, CBH I and CBH II were used as representative enzymes. p-Aminophenyl derivatives of cellobiose (PAPG₂), cellotriose (PAPG₃), and cellotetraose (PAPG₄) were synthesized from the reaction...
Resource Type:: Dissertation
Full Text:: : •- -1 - ■ /- \y - Michael H. Penner A novel type of model substrates, i.e. immobilized p

Separation and detection of cellooligosaccharides on cellulose thin-layer chromatography

Creator:: Sookavatana, Narumon
Abstract:: Linear oligosacchardies of 1,4 linked β-D-glucopyranose are commonly referred to as cellodextrins (CD) or cellooligosaccharides (CO). They are of interest to those working in disciplines involving cellulose chemistry because they are often used as model substrates for cellulose itself. They are of interest to food scientists and nutritionists because they...
Resource Type:: Masters Thesis
Full Text:: approved: Michael H. Penner Linear oligosacchardies of 1,4 linked P-D-glucopyranose are commonly

Preparation and modification of cellooligosaccharides

Creator:: Akpinar, Ozlem
Abstract:: Cellooligosaccharides are the reaction intermediates produced during the hydrolysis of cellulose to glucose. Hence, they have the same chemical structure as cellulose, just shorter chain lengths. Cellooligosaccharides up to DP eight are soluble in water. The soluble cellooligosaccharides can be used "as is" in the food industry as non-digestible oligosaccharides...
Resource Type:: Dissertation
Full Text:: and Modification of Cellooligosaccharides Abstract approved: Michael H. Penner

Salt and polyelectrolyte affect on food colloid function : polygalacturonic acid and egg albumen case studies

Creator:: Chen, Chi-Shen
Abstract:: The primary purpose of this study was to try to (1) provide information on the chemical nature, including turbidity formation, of polygalacturonic acid in different concentrations of NaCl solution. (2) use turbidity formation characteristics to develop an assay for determining the activities polygalacturonic acid depolymerases by using a polyelectrolyte. (3)...
Resource Type:: Dissertation
Full Text:: : ______________________________________________________ Michael. H. Penner The primary purpose of this study was to try to (1) provide

Initial velocity analyses of milling effects on rates of enzyme-catalyzed saccharification of native wheat straw

Creator:: Nguyen, Quynh M.
Abstract:: There is considerable interest in developing environmentally friendly processes for the economical conversion of biomass plant material to biofuels, bioproducts and biomaterials. One approach to such a conversion process is to make use of enzymes for the conversion of plant polysaccharides to simple sugars; the simple sugars could then be...
Resource Type:: Masters Thesis
Full Text:: : _____________________________________________________ Michael H. Penner There is considerable interest in developing environmentally

Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

Creator:: Liaw, Ean-Tun
Abstract:: The influence of substrate and enzyme concentration on the rate of saccharification of two defined, insoluble, cellulose substrates, Avicel and Solka-Floc, by the cellulase enzyme system of Trichoderma viride has been evaluated. Assays utilized enzyme concentrations ranging from 0.014 to 0.056 filter paper unit per mL and substrate concentrations up...
Resource Type:: Masters Thesis
Full Text:: Complex from Trichoderma viride. Abstract approved: , . _ .. ... rr —.—^=— ^ ^ Michael H

Apparent inhibition of Pacific whiting surimi-associated protease by whey protein concentrate

Creator:: Piyachomkwan, Kuakoon
Abstract:: Surimi is a seafood product which is used to manufacture restructured products such as artificial crab and lobster. Surimi is produced from fish fillets by washing to remove sarcoplasmic proteins and increase the concentration of myofibrillar proteins, and mixing with cryoprotectants. A valuable attribute of surimi is its ability to...
Resource Type:: Masters Thesis
Full Text:: approved : . . . . Michael H. Penner, Ph. D. Surimi is a seafood product which is used to

Saccharification and fermentation of lignocellulosic biomass using Trichoderma reesei cellulases and Saccharomyces cerevisiae

Creator:: Chung, Yun-Chin
Abstract:: The efficiency of cellulose hydrolysis under straight saccharification and simultaneous saccharification and fermentation (SSF) conditions was evaluated using three lignocellulosic materials (switchgrass, cornstover, and poplar), which had been pretreated with dilute sulfuric acid under conditions which optimized xylose concentrations in the prehydrolysate liquid. Yields of glucose, cellobiose and ethanol obtained...
Resource Type:: Dissertation
Full Text:: cerevisiae Abstract approved by: Michael H. Penner The efficiency of cellulose hydrolysis under

Cellulase system of Trichoderma reesei QM9414 : a study of its apparent sustrate inhibition

Creator:: Huang, Xiaolin
Resource Type:: Dissertation
Full Text:: Abstract approved by : . Michael H. Penner The apparent substrate inhibition properties of

ScholarsArchive@OSU

Interactions of Trichoderma reesei exo-acting cellulases with p-aminophenyl 1-thio-β-D-cellobioside

Immobilized cellooligosaccharides in the study of trichoderma reesei cellobiohydrolases

Separation and detection of cellooligosaccharides on cellulose thin-layer chromatography

Preparation and modification of cellooligosaccharides

Salt and polyelectrolyte affect on food colloid function : polygalacturonic acid and egg albumen case studies

Initial velocity analyses of milling effects on rates of enzyme-catalyzed saccharification of native wheat straw

Characterization of substrate-velocity relationships for the cellulase enzyme complex from Trichoderma viride

Apparent inhibition of Pacific whiting surimi-associated protease by whey protein concentrate

Saccharification and fermentation of lignocellulosic biomass using Trichoderma reesei cellulases and Saccharomyces cerevisiae

Cellulase system of Trichoderma reesei QM9414 : a study of its apparent sustrate inhibition

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