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  • A (3-Aminopropyl)triethoxysilane (APTES) and Glutaraldehyde Functionalized Cellulose Surface for Immunoassays

    Creator:
    Harley, William
    Abstract:
    The detection of biomolecules is critical for clinical diagnostics but requires specialty labor and high cost. The use of microfluidic paper-based analytical devices (µPADs) for bioassays is simpler and cheaper but has low sensitivity. Improved immobilization of antibodies onto cellulose is demonstrated through surface functionalization with APTES and glutaraldehyde. The...
    Resource Type:
    Honors College Thesis
    Full Text:
    :_____________________________________________________ Vincent Remcho The detection of biomolecules is critical for clinical diagnostics but requires

  • Colorimetric analysis of creatinine in whole blood and urine in a novel paper/polycaprolactone microfluidic device

    Creator:
    Pommerenck, Joel, Heist, Christopher A., and Remcho, Vincent T.
    Abstract:
    A novel approach for the colorimetric detection of creatinine in a paper/polycaprolactone hybrid microfluidic device using alkaline3,5-dinitrobenzoate (DNB) is described. The reaction parameters of reagent concentration, alkalinity, and analysis time are optimized for fast reaction times and high color yield. Microfluidic devices incorporating this assay are designed and fabricated to...
    Resource Type:
    Poster
    Full Text:
    *, Christopher A. Heist†, and Vincent T. Remcho‡ (Primary Author/Undergraduate RA*, Senior Graduate RA†, and PI

  • Improvements in synthesis and response techniques of ion-selective sensors

    Creator:
    Pylypiuk, Nataliia
    Abstract:
    Two improvements to the established procedures for synthesis and response detection of ion-selective optical sensors (optodes) were introduced. The first improvement addresses the drawback of organic dye (optode-localized chromoionophore) photobleaching. This positively impacts fluorescence response and allows for (1) direct measurement of hydrogen ion activity upon binding with the dye,...
    Resource Type:
    Masters Thesis
    Full Text:
    Response Techniques of Ion-Selective Sensors. Abstract approved: Vincent T. Remcho Two

  • Fabrication and application of polymer based microfluidic devices

    Creator:
    Koesdjojo, Myra T.
    Abstract:
    The concept of reducing laboratory operations in scale such that they fit on a microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices promise to be cost effective to operate due to reduced reagent consumption, have the potential to offer shorter analysis times due to their short path lengths,...
    Resource Type:
    Dissertation
    Full Text:
    . CONTRIBUTION OF AUTHORS My research advisor, Dr. Vincent T. Remcho, edited and assisted in writing all