The dairy industry relies primarily on consistent acid production
by the lactic streptococci for the manufacture of certain cheeses
and fermented dairy products. Variation in these cultures due to
genetic exchange has not been thoroughly investigated. This study
was undertaken to determine if genetic homology exists within the lactic
group,...
Fast acid-producing streptomycin-resistant mutants for several
strains of lactic streptococci currently used in commercial Cheddar
cheese manufacture were isolated. Their isolation involved use of a
recently developed differential plating medium that allows identification
of fast acid-producing colonies. Both parents and mutants were characterized
with regard to growth, acid production, proteolytic...
A method for rapid isolation of plasmid DNA from group N
streptococci was developed. Chief advantages of the method were
simplicity, the utilization of microliter quantities of reagents,
and the obtainment of preparations highly enriched for covalently
closed circular plasmid DNA. The method was also applied to over
15 Leuconostoc...
Three commercial sausage starter cultures were grown in
an internal-pH-controlled medium (PHASE 4) and in a soluble
phosphate-buffered medium. The cultures were tested by
comparing viable counts before and after freezing at -40 C
or freeze-drying and subsequent storage for 3 months in the
frozen or dried state. Strains varied...
The purposeful induction of malolactic fermentation (MLF) in wines such as Pinot Noir
and Chardonnay is an established commercial wine making practice in Oregon. This induction
is not always successful, especially with white wines, such as Chardonnay. A study was
initiated to examine the compatibility of yeasts commonly used in...
The fecal flora of healthy bottle or breast-fed infants was
examined for the presence of Bifidobacterium. Identification was
based on the presence of fructose-6-phosphate phosphoketolase,
which is found only in these bacteria. No bifidobacteria were
recovered from bottle-fed infants. However, bifidobacteria were
readily isolated from 15 day to 3 month...