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Aerobic Cometabolism of cis-Dichloroethylene, 1,1,1-Trichloroethane, and 1,4-Dioxane by Polyvinyl Alcohol/Sodium Alginate Hydrogel Beads that Co-Entrapped Rhodococcus rhodochrous Strain ATCC 21198 and Slow-Release Growth Substrates: Column Studies

Creator:: Bailey, Kestrel
Abstract:: CisCis-Dichloroethylene (ciscis-DCE), 1,1,1-Trichloroethane (1,1,1-TCA), and 1,4-Dioxane (1,4-D) are chlorinated solvents used as solvents and degreasers in industrial cleaning agents that have become common groundwater contaminants. To develop an effective treatment method for these compounds, polyvinyl alcohol (PVA) and sodium alginate (SA) hydrogel beads were co-entrappcapsulated with Rhodococcus rhodochrous Strain ATCC...
Resource Type:: Masters Thesis
Full Text:: influent cis-DCE (250 µg/L) and 82% (pseudo-first order rate of 2.27 day-1) of influent 1,4-D (200 µg/L

An anaerobic microcosm study of TCE transformation by microbes stimulated from the Gilbert-Mosley site, Wichita, Kansas

Creator:: Pon, George
Abstract:: A mix-culture of methanogenic and sulfate-reducing bacteria was capable of degrading trichloroethylene (TCE) to dichloroethylene (DCE). The culture was incubated under anaerobic conditions within a soil sample taken from Gilbert-Mosley site, Wichita, Kansas. To stimulate the growth of indigenous bacteria, a carbon and energy source was added in this microcosm...
Resource Type:: Masters Thesis
Full Text:: control to which no substrate was added showed no signs of TCE transformation. Addition of suitable

Aerobic cometabolism of chloroform by butane and propane grown microorganisms from the Hanford subsurface

Creator:: Kim, Young
Abstract:: Batch microcosm studies were carried out to screen for microorganisms from the subsurface of Hanford DOE site that could cometabolically transform chloroform (CF) under aerobic conditions. The potential need for CF bioremediation at the Hanford site has resulted from the large release of carbon tetrachloride (CT) to the subsurface, of...
Resource Type:: Masters Thesis
Full Text:: ammonia was very slow. Microorganisms stimulated on propene and octane showed no ability to transform CF

Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Creator:: Laurance, Jon C.
Abstract:: Chlorinated solvents are common groundwater pollutants that often exist as mixtures resulting from the use of multiple solvents and their transformation into chlorinated daughter products. These sites are often co-contaminated with the solvent stabilizer 1,4-dioxane (1,4-D), which is not treated by traditional chlorinated solvent remediation techniques. Both 1,4-D and chlorinated...
Resource Type:: Masters Thesis
Full Text:: no stimulation of native microorganisms was observed over 50-100 days of incubation. Therefore

Evaluation in a Physical Aquifer Model the Long-Term Performance of a Permeable Reactive Bio-Barrier Packed with Co-encapsulated Gellan Gum Beads for the Bioremediation of Chlorinated Compounds and Co-Contaminants

Creator:: Wortkoetter, Jacob
Abstract:: Chlorinated solvents are a class of widespread groundwater contaminants that are toxic, carcinogenic, and difficult to remediate at low concentrations. 1,4-dioxane, a suspected carcinogen, is often a co-contaminant found in mixtures of chlorinated solvent plumes. In-situ bioremediation methods of degrading these chemicals through aerobic cometabolism have shown promise. Permeable Reactive...
Resource Type:: Masters Thesis
Full Text:: . Bromide added as a conservative tracer indicated no significant changes in the PAM groundwater flow

Development and applications of a high-throughput activity assay, using the ammonia-oxidizing bacteria Nitrosomonas europaea

Creator:: Bartow, Shannon M.
Abstract:: The rising demand on freshwater resources emphasizes the benefit of biogeochemical processes that maintain clean water by removing pollutants such as excess nitrogen. The most sensitive step in the removal of nitrogen from waterways is the oxidation of ammonia, a part of nitrification, which is carried out by ammonia oxidizing...
Resource Type:: Masters Thesis
Full Text:: conferred protection against inhibition to AgNPs, little or no protection was seen when cells were exposed

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Pure Cultures of Rhodococcus rhodochrous 21198 and Mycobacterium ELW1 and in Groundwater Microcosms Fed Isobutane and Isobutene as Growth Substrates

Creator:: Krippaehne, Krysta J.
Abstract:: 1,4-dioxane and chlorinated aliphatic hydrocarbons (CAHs) such as trichloroethylene (TCE) and 1,1-dichloroethene (1,1-DCE) are hazardous compounds commonly found in soil and groundwater. Bioremediation through aerobic cometabolism is a potential option for the remediation of these contaminated sites. The purpose of this study was to examine the use of different primary...
Resource Type:: Masters Thesis
Full Text:: , no primary substrate uptake and minimal TCE and 1,1-DCE degradation were observed. It is

Inhibition of the ammonia oxidizing bacteria Nitrosomonas europaea by the emerging contaminant triclosan

Creator:: Hughes, Jamie L.
Abstract:: Identifying the inhibition of ammonia oxidizing bacteria (AOB) by emerging organic contaminants is crucial due to the importance of AOB in wastewater treatment, the widespread use of antibacterial agents such as triclosan (TCS) in consumer products, and the sensitivity of N. europaea to inhibitors. Triclosan inhibition of nitrification by AOB...
Resource Type:: Masters Thesis
Full Text:: cell growth. Resistance tests showed no development in resistance to 0.01 ppm TCS over three growth

Aerobic Cometabolism of 1,4-Dioxane and cis-Dichloroethylene by Gellan Gum and Polyvinyl Alcohol/Sodium Alginate Hydrogel Beads Co-Encapsulated with Rhodococcus rhodochrous Strain ATCC 21198 and Slow-Release Growth Substrates: Batch, Column, and Toxicity Studies

Creator:: Conway, Sean P.
Abstract:: 1,4-Dioxane (dioxane) and cis-dichloroethylene (cDCE) are compounds commonly found in industrial cleaning and degreasing agents that are frequently present as groundwater contaminants. In an effort to develop a more effective treatment method for these compounds, hydrogel beads were fabricated with either gellan gum or a combination of polyvinyl alcohol (PVA)...
Resource Type:: Masters Thesis
Full Text:: completed using the TBOS gellan gum beads showed no significant toxicity was generated after the complete

Aerobic degradation of chlorinated ethenes by Mycobacterium strain JS60 in the presence of organic acids

Creator:: Blatchford, Christina
Abstract:: This study evaluated the potential of the aerobic Mycobacterium strain JS6O to grow on a variety of organic acid substrates, and the possible effects an organic acid would have on the degradation rate of vinyl chloride (VC). A series of batch growth tests were designed to determine the time it...
Resource Type:: Masters Thesis
Full Text:: presence of c-DCE was shown to partially inhibit VC degradation, but had no effect on ethylene degradation

ScholarsArchive@OSU

Aerobic Cometabolism of cis-Dichloroethylene, 1,1,1-Trichloroethane, and 1,4-Dioxane by Polyvinyl Alcohol/Sodium Alginate Hydrogel Beads that Co-Entrapped Rhodococcus rhodochrous Strain ATCC 21198 and Slow-Release Growth Substrates: Column Studies

An anaerobic microcosm study of TCE transformation by microbes stimulated from the Gilbert-Mosley site, Wichita, Kansas

Aerobic cometabolism of chloroform by butane and propane grown microorganisms from the Hanford subsurface

Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Evaluation in a Physical Aquifer Model the Long-Term Performance of a Permeable Reactive Bio-Barrier Packed with Co-encapsulated Gellan Gum Beads for the Bioremediation of Chlorinated Compounds and Co-Contaminants

Development and applications of a high-throughput activity assay, using the ammonia-oxidizing bacteria Nitrosomonas europaea

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Pure Cultures of Rhodococcus rhodochrous 21198 and Mycobacterium ELW1 and in Groundwater Microcosms Fed Isobutane and Isobutene as Growth Substrates

Inhibition of the ammonia oxidizing bacteria Nitrosomonas europaea by the emerging contaminant triclosan

Aerobic Cometabolism of 1,4-Dioxane and cis-Dichloroethylene by Gellan Gum and Polyvinyl Alcohol/Sodium Alginate Hydrogel Beads Co-Encapsulated with Rhodococcus rhodochrous Strain ATCC 21198 and Slow-Release Growth Substrates: Batch, Column, and Toxicity Studies

Aerobic degradation of chlorinated ethenes by Mycobacterium strain JS60 in the presence of organic acids

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