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Microcosm studies of bioaugmentation with a butane-utilizing mixed culture : microbial community structure and 1,1-DCE cometabolism

Creator:: Lim, Hee Kyung
Abstract:: The 1,1-dichloroethene (1,1-DCE) cometabolic transformation abilities of indigenous and bioaugmented microorganisms were compared in microcosms constructed with groundwater and aquifer solids from the Moffett Field site, CA. Microbial community structure in the microcosms and possible community shifts due to 1,1-DCE transformation stress was evaluated by terminal restriction fragment length polymorphism...
Resource Type:: Masters Thesis
Full Text:: . Robert W. Collier for serving as Graduate Council Representative on my committee. It was very lucky for

Co-Encapsulation of Slow Release Substrates and Microbial Cultures in Alginate and Gellan Gum Beads to Promote the Co-metabolic Transformation of 1,4-Dioxane and Chlorinate Aliphatic Hydrocarbons

Creator:: Rasmussen, Mitchell T.
Abstract:: Methods were developed for the co-encapsulation of slow release compounds (SRC) with viable microbial cells in alginate and gellan gum hydrogel beads, for the in-situ aerobic cometabolic treatment of groundwater contaminated with mixtures of 1,4-dioxane and chlorinated aliphatic hydrocarbons, which will be referred to as contaminants of concern (CoC). The...
Resource Type:: Masters Thesis
Full Text:: high mass loadings, >10% (w/w), and co-encapsulated ATCC 21198 was shown to be able to consume SRC

Cometabolism of Chlorinated Ethenes by Burkholderia vietnamiensis G4 Grown on Aromatic Substrates: Resting and Co-encapsulated Cell Kinetic Tests

Creator:: Saito, Alyssa M.
Abstract:: The cometabolic ability of Burkholderia vietnamiensis G4 grown on aromatic substrates was first assessed by resting suspended cells, and subsequently for cells encapsulated with and without slow release compounds (SRCs). In Chapter 3, benzyl alcohol was assessed as a food-grade growth substrate for promoting cometabolism of trichloroethene (TCE), via the...
Resource Type:: Masters Thesis
Full Text:: for in situ use. TPhOS, which hydrolyzes to produce phenol, was co-encapsulated at 1.9% (w/w) and

Aerobic biotransformation of chlorinated aliphatic hydrocarbons by a benzyl alcohol grown mixed culture : cometabolism, mechanisms, kinetics and modeling

Creator:: Tejasen, Sarun
Abstract:: The aerobic transformation of TCE and cis-DCE by a tetrabutoxysilane-grown microorganism (Vancheeswaran et al., 1999) led to the investigation of novel substrates, including benzyl alcohol, for promoting cometabolism. The culture grew on carboxylic compounds and alcohols, but did not grow on formate, methanol, methane, propane, butane, ethylene, benzene, toluene, or...
Resource Type:: Dissertation
Full Text:: . 58(9), 3038-3 046. Ewers, 3., Clemens, W., and Knackmuss, H. J., Eds. (1991). Biodegradation of

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Pure Cultures of Rhodococcus rhodochrous 21198 and Mycobacterium ELW1 and in Groundwater Microcosms Fed Isobutane and Isobutene as Growth Substrates

Creator:: Krippaehne, Krysta J.
Abstract:: 1,4-dioxane and chlorinated aliphatic hydrocarbons (CAHs) such as trichloroethylene (TCE) and 1,1-dichloroethene (1,1-DCE) are hazardous compounds commonly found in soil and groundwater. Bioremediation through aerobic cometabolism is a potential option for the remediation of these contaminated sites. The purpose of this study was to examine the use of different primary...
Resource Type:: Masters Thesis
Full Text:: , textile processing, and coating solvents (Ward and Stroo, 2010). These solvents gained popularity due

Transient Electron Donor Concentration Experiments for the Determination of Dehalogenation Rate and Kinetic Parameter Shifts in an Anaerobic Microbial Culture

Creator:: Green, Jennifer K.
Abstract:: Trichloroethene (TCE) is a common groundwater contaminant. Bioremediation, or the enhancement of natural microbial processes for the transformation of toxic compounds in soil and groundwater, is an effective solution to this widespread problem. The organisms that transform TCE in the subsurface are sensitive to environmental conditions, especially to the presence...
Resource Type:: Honors College Thesis

Anaerobic Dehalogenation of Trichloroethene by Encapsulated Dehalococcoides mccartyi

Creator:: Lukens, Eileen P.
Abstract:: Chlorinated aliphatic hydrocarbons (CAHs) like trichloroethene (TCE) were mostly used to replace drying cleaning fluids which contained hydrocarbons like benzene, and were highly flammable. Through improper disposal, storage, and spills, TCE and its downstream products, like cis-dichloroethene (cDCE) and vinyl chloride (VC), leached into groundwater and threaten human and environmental...
Resource Type:: Honors College Thesis

Inhibition of Organohalide-Respiring Bacteria by Carbon Tetrachloride and Chloroform

Creator:: Ehret, Emma L.
Abstract:: Despite decades of cleanup efforts, chlorinated solvents are some of the most common groundwater and subsurface contaminants of the industrialized world. These compounds include chlorinated ethenes (CEs) such as trichloroethene (TCE) and chlorinated methanes (CMs) such as carbon tetrachloride (CT). Dehalococcoides mccartyi belongs to a class of microorganisms called organohalide-respiring...
Resource Type:: Masters Thesis

Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Creator:: Laurance, Jon C.
Abstract:: Chlorinated solvents are common groundwater pollutants that often exist as mixtures resulting from the use of multiple solvents and their transformation into chlorinated daughter products. These sites are often co-contaminated with the solvent stabilizer 1,4-dioxane (1,4-D), which is not treated by traditional chlorinated solvent remediation techniques. Both 1,4-D and chlorinated...
Resource Type:: Masters Thesis

Numerical simulation of anaerobic reductive dechlorination of CAHs in continuous flow systems

Creator:: Mustafa, Nizar Ahmad
Abstract:: Halogenated organic compounds have had widespread and massive applications in industry, agriculture, and private households, for example, as degreasing solvents, flame retardants and in polymer production. They are released to the environment through both anthropogenic and natural sources. The most common chlorinated solvents present as contaminants include tetrachloroethene (PCE, perchloroethene)...
Resource Type:: Dissertation

ScholarsArchive@OSU

Microcosm studies of bioaugmentation with a butane-utilizing mixed culture : microbial community structure and 1,1-DCE cometabolism

Co-Encapsulation of Slow Release Substrates and Microbial Cultures in Alginate and Gellan Gum Beads to Promote the Co-metabolic Transformation of 1,4-Dioxane and Chlorinate Aliphatic Hydrocarbons

Cometabolism of Chlorinated Ethenes by Burkholderia vietnamiensis G4 Grown on Aromatic Substrates: Resting and Co-encapsulated Cell Kinetic Tests

Aerobic biotransformation of chlorinated aliphatic hydrocarbons by a benzyl alcohol grown mixed culture : cometabolism, mechanisms, kinetics and modeling

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Pure Cultures of Rhodococcus rhodochrous 21198 and Mycobacterium ELW1 and in Groundwater Microcosms Fed Isobutane and Isobutene as Growth Substrates

Transient Electron Donor Concentration Experiments for the Determination of Dehalogenation Rate and Kinetic Parameter Shifts in an Anaerobic Microbial Culture

Anaerobic Dehalogenation of Trichloroethene by Encapsulated Dehalococcoides mccartyi

Inhibition of Organohalide-Respiring Bacteria by Carbon Tetrachloride and Chloroform

Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Numerical simulation of anaerobic reductive dechlorination of CAHs in continuous flow systems

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