Index Catalog // ScholarsArchive@OSU

Numerical simulation of anaerobic reductive dechlorination of CAHs in continuous flow systems

Creator:: Mustafa, Nizar Ahmad
Abstract:: Halogenated organic compounds have had widespread and massive applications in industry, agriculture, and private households, for example, as degreasing solvents, flame retardants and in polymer production. They are released to the environment through both anthropogenic and natural sources. The most common chlorinated solvents present as contaminants include tetrachloroethene (PCE, perchloroethene)...
Resource Type:: Dissertation
Full Text:: . committee members, Mark Dolan, Jack Istok, Brian Wood and John Baham for his role as Graduate Council

Correlation of NE 1545 expression and cell size in Nitrosomonas europaea exposed to a suite of aromatic hydrocarbons

Creator:: Gilroy, Caslin Anne
Abstract:: Ammonia oxidizing bacteria, including Nitrosomonas europaea, are inhibited by aromatic hydrocarbons which can be found in wastewater treatment plants. In recent studies, changes have been observed in N. europaea cell size upon exposure to benzene, but not toluene. Additionally, NE 1545, a gene proposed to be involved in fatty acid...
Resource Type:: Honors College Thesis
Full Text:: expression were measured. It was found that exposure to compounds with a dipole moment of 1.5 D and greater

Transformation of Carbon Tetrachloride and Chloroform by Trichloroethene Respiring Anaerobic Mixed Cultures and Supernatant

Creator:: Vickstrom, Kyle E.
Abstract:: Carbon tetrachloride (CT) and chloroform (CF) were transformed in batch reactor experiments conducted with anaerobic dechlorinating cultures and supernatant (ADC+S) harvested from continuous flow reactors. The Evanite (EV-5L) and Victoria/Stanford (VS-5L) cultures capable of respiring trichloroethene (TCE), 1,2-cis-dichloroethene (cDCE), and vinyl chloride (VC) to ethene (ETH) were grown in continuous...
Resource Type:: Masters Thesis
Full Text:: Radniecki, Mark Dolan, and Jack Istok. You all have encouraged critical thinking and deeper analysis in

Electron donor and chlorinated ethene effects on activity and community composition in anaerobic reductively dechlorinating consortia

Creator:: Sabalowsky, Andrew R.
Abstract:: This research focused on anaerobic transformation of trichloroethene (TCE), a groundwater contaminant. The mixed anaerobic Evanite culture (EV) was studied to determine community behavior and composition responses to different electron donors and chloroethene electron acceptors. The potential toxicity from high concentrations of TCE and its daughter product cis-1,2-dichloroethene (cDCE) was...
Resource Type:: Dissertation
Full Text:: like to express deep gratitude to the members of my advising committee, Drs. Peter Bottomley, Mark

Experimental demonstration and modeling of aerobic cometabolism of 1,4-dioxane by isobutane-utilizing microorganisms in aquifer microcosms

Creator:: Rolston, Hannah M.
Abstract:: 1,4-dioxane, a probable human carcinogen at low (< 1ppb) concentrations, has emerged as a groundwater contaminant due to its historical use as a stabilizer for the chlorinated solvent 1,1,1-trichloroethane. Aerobic cometabolism, the use of a primary substrate to induce the production of microbial enzymes that fortuitously degrade other compounds, is...
Resource Type:: Masters Thesis
Full Text:: this thesis would not accomplished. I would also like to thank my committee members: Dr. Mark

The effect of silver ions and nanoparticles on biofilms and planktonic cultures of Nitrosomonas europaea

Creator:: Giska, Jonathan R.
Abstract:: Due to the effective antimicrobial properties of silver nanoparticles they represent a significant proportion of all consumer-‐related nanomaterials. The introduction of silver nanoparticles from these products into domestic and industrial wastewater streams poses potential complications for biological treatment systems. A critical component of biological treatment that is extremely sensitive to...
Resource Type:: Masters Thesis
Full Text:: of the nanoparticles. The free electrons (d-‐orbital) of silver are

Inhibition, gene expression and modeling of ammonia oxidation in biofilms of Nitrosomonas europaea

Creator:: Lauchnor, Ellen Grace
Abstract:: This dissertation explores the physiology and gene expression of the ammonia-oxidizing bacterium Nitrosomonas europaea in surface-associated bacterial communities, or biofilms. Biofilms of N. europaea were cultivated in drip flow reactors for several weeks and gene expression microarrays were used to detect 240 genes differentially expressed between the mature biofilms and...
Resource Type:: Dissertation
Full Text:: with microsensors and the concentration profiles were simulated with 2-D reactive transport that

Effect of electron donors on the reductive dechlorination of trichlorofluoroethylene in anaerobic microcosms and in situ push-pull tests

Creator:: Louie, Erika N.
Abstract:: This research focuses on the anaerobic transformation of trichloroethylene (TCE) that is occurring in the subsurface of the Evanite site in Corvallis, OR. Two anaerobic studies were conducted to investigate the effects of microbial communities in the presence of different electron donors during the anaerobic degradation of trichlorofluoroethylene (TCFE), and...
Resource Type:: Masters Thesis
Full Text:: lene · Anaerobic Microcosms and In Situ Push-Pull Tests. Abstract approved: Jonathan D. Istok

The Effect of Oxygen and Salinity Conditions on Soluble Metabolic Product Formation by Methylomicrobium buryatense 5GB1

Creator:: Cameron, Marina R.
Abstract:: Methanotrophic bacteria are promising means of producing value-added products as they have the ability to transform methane under atmospheric temperature and pressure. Efforts to develop methanotrophs to produce value-added products are hoped to incentivize a decrease in methane flaring operations. The data collected during this study was used to inform...
Resource Type:: Masters Thesis
Full Text:: . Besides my advisor, I would like to thank the rest of my thesis committee: Mark Dolan, Kate Schilke

Inhibition of Organohalide-Respiring Bacteria by Carbon Tetrachloride and Chloroform

Creator:: Ehret, Emma L.
Abstract:: Despite decades of cleanup efforts, chlorinated solvents are some of the most common groundwater and subsurface contaminants of the industrialized world. These compounds include chlorinated ethenes (CEs) such as trichloroethene (TCE) and chlorinated methanes (CMs) such as carbon tetrachloride (CT). Dehalococcoides mccartyi belongs to a class of microorganisms called organohalide-respiring...
Resource Type:: Masters Thesis
Full Text:: . Next, a big thank you to my committee members: Drs. Tyler Radniecki, Mark Dolan, and Jack Istok and

Inhibition of the ammonia oxidizing bacteria Nitrosomonas europaea by the emerging contaminant triclosan

Creator:: Hughes, Jamie L.
Abstract:: Identifying the inhibition of ammonia oxidizing bacteria (AOB) by emerging organic contaminants is crucial due to the importance of AOB in wastewater treatment, the widespread use of antibacterial agents such as triclosan (TCS) in consumer products, and the sensitivity of N. europaea to inhibitors. Triclosan inhibition of nitrification by AOB...
Resource Type:: Masters Thesis
Full Text:: growth of a 10% cell density inoculum of N. europaea and (D) cell growth, (E) nitrite production and (F

Kinetic and molecular effects of sulfate reduction on a dechlorinating culture under chemostat growth conditions

Creator:: Berggren, Dusty Rose V.
Abstract:: Chlorinated ethenes are common groundwater contaminants that may be treated through in-situ bioremediation. Relationships between the reducing environment, available electron donors and acceptors, reaction kinetics, and microbial community composition must be further understood to successfully engineer remediation schemes in the complex subsurface environment. This thesis work investigated the effect of...
Resource Type:: Masters Thesis
Full Text:: experiments gone awry enhanced all of the hours spent in the lab. I would also like to thank Mark Dolan for

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Pure Cultures of Rhodococcus rhodochrous 21198 and Mycobacterium ELW1 and in Groundwater Microcosms Fed Isobutane and Isobutene as Growth Substrates

Creator:: Krippaehne, Krysta J.
Abstract:: 1,4-dioxane and chlorinated aliphatic hydrocarbons (CAHs) such as trichloroethylene (TCE) and 1,1-dichloroethene (1,1-DCE) are hazardous compounds commonly found in soil and groundwater. Bioremediation through aerobic cometabolism is a potential option for the remediation of these contaminated sites. The purpose of this study was to examine the use of different primary...
Resource Type:: Masters Thesis
Full Text:: . Mark Dolan, and Dr. Jack Istok, for taking the time to review my thesis, be present at my defense

Batch and Continuous Flow Column Studies of Methane Consumption and Methanol Production by Methylosinus Trichosporium OB3b and Methylomicrobium Buryatense 5GB1 Immobilized In Ca-Alginate and Agarose Hydrogels

Creator:: Molzahn, Paige
Abstract:: Two methanotrophs, M. trichosporium OB3b and M. buryatense 5GB1, were encapsulated using two methods to investigate the potential of methane conversion for biofuel production. Ca-alginate and low melt agarose were used to immobilize the methanotrophs for batch and continuous flow column testing. Varying protein concentrations, residence times, and immobilization methods...
Resource Type:: Masters Thesis
Full Text:: gratitude to the members of my committee; Dr. Mark Dolan, Dr. Karl Schilke, and Dr. Jack Istok. Mark and

Abiotic and biological transformation of TBOS and TKEBS, and their role in the biological transformation of TCE and c-DCE

Creator:: Vancheeswaran, Sanjay
Abstract:: At Site-300, Lawrence Livermore National Laboratory (LLNL), CA, trichloroethene (TCE) is present along with tetraalkoxysilanes such as tetrabutoxysilane (TBOS) and tetrakis(2-ethylbutoxy) silane (TKEBS), as subsurface contaminants. Intrinsic transformation of TCE to cis-dichloroethene (c-DCE) was observed in the groundwater at locations co-contaminated with TBOS or TKEBS. Attenuation of TBOS and TKEBS...
Resource Type:: Masters Thesis
Full Text:: , Ying, Lutz, George, Matt, Jason, Merf, Young, Sarun, Pardi, Dave, Mark, Mohammad) who have

Kinetic and modeling investigations of the anaerobic reductive dechlorination of chlorinated ethylenes using single and binary mixed cultures and silicon-based organic compounds as slow-release substrates

Creator:: Yu, Seungho
Abstract:: This study investigated complete reductive dechlorination of chlorinated aliphatic hydrocarbons (CAHs) over a broad range of concentrations. Tetrabutoxysilane (TBOS), as a slow-release anaerobic substrate, was studied for enhanced reductive dechlorination of tetrachioroethylene (PCE) present as a dense non-aqueous liquid (DNAPL). Four different site-mixed cultures were used in the study: Site-300...
Resource Type:: Dissertation
Full Text:: my committee members Dr. Charles Bruimer, Dr Mark Dolan, Dr. Jack Istok, and Dr Kenneth Williamson

Factors controlling halogenated and nonhalogenated alkene growth substrate range of vinyl chloride-utilizing bacteria

Creator:: Taylor, Anne Elizabeth
Abstract:: This thesis explores the factors limiting the alkene substrate range of the vinyl chloride (VC)-utilizing bacteria, and describes a method for measuring VC transformation in situ. Vinyl fluoride (VF) was evaluated as a surrogate for monitoring aerobic VC-transformation utilizing three isolates, Mycobacterium EE13a, Mycobacterium JS60 and Nocardioides JS614. JS614 grew...
Resource Type:: Dissertation
Full Text:: committee for their time and attention. Dan Arp for his many insightful comments in lab meeting, Mark

Effects of silver ions and nanoparticles on suspended cells and biofilms of Nitrosomonas europaea

Creator:: Barker, Leila Kelly
Abstract:: Silver nanoparticles are increasingly being incorporated into consumer products due to their broad-spectrum antimicrobial properties. The resulting influx of silver nanoparticles into wastewater may pose a threat to bacteria involved in biological wastewater treatment. Ammonia-oxidizing bacteria, which convert ammonia to nitrite in the first step of nitrification, are highly sensitive...
Resource Type:: Masters Thesis
Full Text:: and techniques. Andy Ungerer is an ICP wizard. Dr. Adam Higgins and Dr. Mark Dolan allowed me to use

Kinetic analysis of the aerobic degradation of chlorinated ethenes by the mycobacterium ELW-1 and chlorinated ethanes and ethenes by rhodococcus rhodochrous ATCC® 21198™

Creator:: Rich, Stephanie L.
Abstract:: Bioremediation of chlorinated ethenes by the augmentation of contaminated soils with microorganisms is a proposed method of reducing contaminant concentrations in groundwater. To determine the viability of using ELW-1 or R. rhodochrous for groundwater remediation, kinetic analyses of transformation rates must be performed. The focus of this research was to...
Resource Type:: Honors College Thesis
Full Text:: µmol/mg TSS-d for vinyl chloride (VC), 0.2 µmol/mg TSS-d for cis-Dichloroethene (c-DCE), and 4.1 µmol

Modeling cometabolic transformation of a CAH mixture by a butane utilizing culture

Creator:: Mathias, Maureen Anne
Abstract:: The goal of this research was to mathematically simulate the ability of bioaugmented microorganisms to aerobically cometabolize a mixture of chlorinated aliphatic hydrocarbon (CAH) compounds during in-situ treatment. Parameter values measured from laboratory experiments were applied to the transport model with biotransformation processes included. In laboratory microcosm studies, a butane-grown,...
Resource Type:: Masters Thesis
Full Text:: biotransformation model was combined with 1 -D advective-dispersive transport to simulate behavior of the culture

ScholarsArchive@OSU

Numerical simulation of anaerobic reductive dechlorination of CAHs in continuous flow systems

Correlation of NE 1545 expression and cell size in Nitrosomonas europaea exposed to a suite of aromatic hydrocarbons

Transformation of Carbon Tetrachloride and Chloroform by Trichloroethene Respiring Anaerobic Mixed Cultures and Supernatant

Electron donor and chlorinated ethene effects on activity and community composition in anaerobic reductively dechlorinating consortia

Experimental demonstration and modeling of aerobic cometabolism of 1,4-dioxane by isobutane-utilizing microorganisms in aquifer microcosms

The effect of silver ions and nanoparticles on biofilms and planktonic cultures of Nitrosomonas europaea

Inhibition, gene expression and modeling of ammonia oxidation in biofilms of Nitrosomonas europaea

Effect of electron donors on the reductive dechlorination of trichlorofluoroethylene in anaerobic microcosms and in situ push-pull tests

The Effect of Oxygen and Salinity Conditions on Soluble Metabolic Product Formation by Methylomicrobium buryatense 5GB1

Inhibition of Organohalide-Respiring Bacteria by Carbon Tetrachloride and Chloroform

Inhibition of the ammonia oxidizing bacteria Nitrosomonas europaea by the emerging contaminant triclosan

Kinetic and molecular effects of sulfate reduction on a dechlorinating culture under chemostat growth conditions

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Pure Cultures of Rhodococcus rhodochrous 21198 and Mycobacterium ELW1 and in Groundwater Microcosms Fed Isobutane and Isobutene as Growth Substrates

Batch and Continuous Flow Column Studies of Methane Consumption and Methanol Production by Methylosinus Trichosporium OB3b and Methylomicrobium Buryatense 5GB1 Immobilized In Ca-Alginate and Agarose Hydrogels

Abiotic and biological transformation of TBOS and TKEBS, and their role in the biological transformation of TCE and c-DCE

Kinetic and modeling investigations of the anaerobic reductive dechlorination of chlorinated ethylenes using single and binary mixed cultures and silicon-based organic compounds as slow-release substrates

Factors controlling halogenated and nonhalogenated alkene growth substrate range of vinyl chloride-utilizing bacteria

Effects of silver ions and nanoparticles on suspended cells and biofilms of Nitrosomonas europaea

Kinetic analysis of the aerobic degradation of chlorinated ethenes by the mycobacterium ELW-1 and chlorinated ethanes and ethenes by rhodococcus rhodochrous ATCC® 21198™

Modeling cometabolic transformation of a CAH mixture by a butane utilizing culture

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