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Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Creator:: Laurance, Jon C.
Abstract:: Chlorinated solvents are common groundwater pollutants that often exist as mixtures resulting from the use of multiple solvents and their transformation into chlorinated daughter products. These sites are often co-contaminated with the solvent stabilizer 1,4-dioxane (1,4-D), which is not treated by traditional chlorinated solvent remediation techniques. Both 1,4-D and chlorinated...
Resource Type:: Masters Thesis
Full Text:: AN ABSTRACT OF THE THESIS OF Jon C. Laurance for

Anaerobic Dehalogenation of Trichloroethene by Encapsulated Dehalococcoides mccartyi

Creator:: Lukens, Eileen P.
Abstract:: Chlorinated aliphatic hydrocarbons (CAHs) like trichloroethene (TCE) were mostly used to replace drying cleaning fluids which contained hydrocarbons like benzene, and were highly flammable. Through improper disposal, storage, and spills, TCE and its downstream products, like cis-dichloroethene (cDCE) and vinyl chloride (VC), leached into groundwater and threaten human and environmental...
Resource Type:: Honors College Thesis
Full Text:: , and Jon Laurance for countless laughs and Alyssa Saito, Emma Ehret, Marina Cameron, and Krysta

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Pure Cultures of Rhodococcus rhodochrous 21198 and Mycobacterium ELW1 and in Groundwater Microcosms Fed Isobutane and Isobutene as Growth Substrates

Creator:: Krippaehne, Krysta J.
Abstract:: 1,4-dioxane and chlorinated aliphatic hydrocarbons (CAHs) such as trichloroethylene (TCE) and 1,1-dichloroethene (1,1-DCE) are hazardous compounds commonly found in soil and groundwater. Bioremediation through aerobic cometabolism is a potential option for the remediation of these contaminated sites. The purpose of this study was to examine the use of different primary...
Resource Type:: Masters Thesis
Full Text:: forever owe a huge debt of gratitude to Jon Laurance for his support with this project. This thesis

Co-Encapsulation of Slow Release Substrates and Microbial Cultures in Alginate and Gellan Gum Beads to Promote the Co-metabolic Transformation of 1,4-Dioxane and Chlorinate Aliphatic Hydrocarbons

Creator:: Rasmussen, Mitchell T.
Abstract:: Methods were developed for the co-encapsulation of slow release compounds (SRC) with viable microbial cells in alginate and gellan gum hydrogel beads, for the in-situ aerobic cometabolic treatment of groundwater contaminated with mixtures of 1,4-dioxane and chlorinated aliphatic hydrocarbons, which will be referred to as contaminants of concern (CoC). The...
Resource Type:: Masters Thesis
Full Text:: Rolston, Riley Murnane, Krysta Krippaehne, Alyssa Saito, Jon Laurance, Gillian Williams, and Grant

Factors controlling halogenated and nonhalogenated alkene growth substrate range of vinyl chloride-utilizing bacteria

Creator:: Taylor, Anne Elizabeth
Abstract:: This thesis explores the factors limiting the alkene substrate range of the vinyl chloride (VC)-utilizing bacteria, and describes a method for measuring VC transformation in situ. Vinyl fluoride (VF) was evaluated as a surrogate for monitoring aerobic VC-transformation utilizing three isolates, Mycobacterium EE13a, Mycobacterium JS60 and Nocardioides JS614. JS614 grew...
Resource Type:: Dissertation
Full Text:: of VF transformation and F- accumulation were correlated with the rate of VC transformation through

Encapsulation of Rhodococcus rhodochrous ATCC 21198 in gellan gum at high densities and with a slow-release substrate for the in-situ bioremediation of contaminant mixtures from groundwater

Creator:: Williams, Gillian R.
Abstract:: As access to potable water grows scarce, there is an increasing reliance on alternative drinking water sources, namely groundwater (WHO, 2018). Chlorinated solvents, such as 1,1,1-trichloroethane (TCA) and 1,2-cis-Dichloroethene (cisDCE) are some of the most ubiquitous groundwater contaminants spread throughout the US. 1,4-dioxane (1,4D) was used primarily in the late...
Resource Type:: Honors College Thesis
Full Text:: and analysis. Thank you to Hannah Rolston, Jon Laurance, and Riley Murnane for their guidance and

Cometabolism of Chlorinated Ethenes by Burkholderia vietnamiensis G4 Grown on Aromatic Substrates: Resting and Co-encapsulated Cell Kinetic Tests

Creator:: Saito, Alyssa M.
Abstract:: The cometabolic ability of Burkholderia vietnamiensis G4 grown on aromatic substrates was first assessed by resting suspended cells, and subsequently for cells encapsulated with and without slow release compounds (SRCs). In Chapter 3, benzyl alcohol was assessed as a food-grade growth substrate for promoting cometabolism of trichloroethene (TCE), via the...
Resource Type:: Masters Thesis
Full Text:: Rolston, Mitchell Rasmussen, Riley Murnane, Jon Laurence, Alisa Bealessio, Brad Jones, Eileen Lukens

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Rhodococcus rhodochrous ATCC 21198 and Coencapsulated with a Slow-Release Substrate in Microcosm and Column Studies

Creator:: Bealessio, Alisa D.
Abstract:: Mixtures of 1,4-dioxane (1,4-D) and chlorinated aliphatic hydrocarbons (CAHs) are common and hazardous groundwater contaminants that can be challenging to address with traditional remediation strategies. Bioremediation through aerobic cometabolism has the potential to be an effective in-situ treatment strategy for these contaminants. Rhodococcus rhodochrous strain ATCC 21198 is capable of...
Resource Type:: Masters Thesis
Full Text:: other graduate students who I have been lucky to work with in the Semprini lab group. Jon Laurence and

Modeling cometabolic transformation of a CAH mixture by a butane utilizing culture

Creator:: Mathias, Maureen Anne
Abstract:: The goal of this research was to mathematically simulate the ability of bioaugmented microorganisms to aerobically cometabolize a mixture of chlorinated aliphatic hydrocarbon (CAH) compounds during in-situ treatment. Parameter values measured from laboratory experiments were applied to the transport model with biotransformation processes included. In laboratory microcosm studies, a butane-grown,...
Resource Type:: Masters Thesis
Full Text:: to come. Thank you to Dr. Jon Kimerling for acting as graduate representative on my committee

Continuous-flow and batch column studies of anaerobic carbon tetrachloride biotransformation on Hanford soil

Creator:: Niemet, Michael Richard
Abstract:: Continuous-flow and batch experiments were conducted with a column reactor system containing Hanford aquifer material in order to evaluate the potential of in-situ bioremediation of carbon tetrachloride (CT) at Hanford. The effectiveness of benzoate and acetate as primary substrates was considered. Nitrate and sulfate were potential electron acceptors. Transport experiments...
Resource Type:: Masters Thesis
Full Text:: APPENDIX F: Basic Concepts 121 APPENDIX G: Protocol for Anaerobic Column Packing 126 Solutions 128

ScholarsArchive@OSU

Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Anaerobic Dehalogenation of Trichloroethene by Encapsulated Dehalococcoides mccartyi

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Pure Cultures of Rhodococcus rhodochrous 21198 and Mycobacterium ELW1 and in Groundwater Microcosms Fed Isobutane and Isobutene as Growth Substrates

Co-Encapsulation of Slow Release Substrates and Microbial Cultures in Alginate and Gellan Gum Beads to Promote the Co-metabolic Transformation of 1,4-Dioxane and Chlorinate Aliphatic Hydrocarbons

Factors controlling halogenated and nonhalogenated alkene growth substrate range of vinyl chloride-utilizing bacteria

Encapsulation of Rhodococcus rhodochrous ATCC 21198 in gellan gum at high densities and with a slow-release substrate for the in-situ bioremediation of contaminant mixtures from groundwater

Cometabolism of Chlorinated Ethenes by Burkholderia vietnamiensis G4 Grown on Aromatic Substrates: Resting and Co-encapsulated Cell Kinetic Tests

Cometabolism of 1,4-Dioxane and Chlorinated Aliphatic Hydrocarbons by Rhodococcus rhodochrous ATCC 21198 and Coencapsulated with a Slow-Release Substrate in Microcosm and Column Studies

Modeling cometabolic transformation of a CAH mixture by a butane utilizing culture

Continuous-flow and batch column studies of anaerobic carbon tetrachloride biotransformation on Hanford soil

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