Index Catalog // ScholarsArchive@OSU

An anaerobic microcosm study of TCE transformation by microbes stimulated from the Gilbert-Mosley site, Wichita, Kansas

Creator:: Pon, George
Abstract:: A mix-culture of methanogenic and sulfate-reducing bacteria was capable of degrading trichloroethylene (TCE) to dichloroethylene (DCE). The culture was incubated under anaerobic conditions within a soil sample taken from Gilbert-Mosley site, Wichita, Kansas. To stimulate the growth of indigenous bacteria, a carbon and energy source was added in this microcosm...
Resource Type:: Masters Thesis
Full Text:: control to which no substrate was added showed no signs of TCE transformation. Addition of suitable

Aerobic cometabolism of chloroform by butane and propane grown microorganisms from the Hanford subsurface

Creator:: Kim, Young
Abstract:: Batch microcosm studies were carried out to screen for microorganisms from the subsurface of Hanford DOE site that could cometabolically transform chloroform (CF) under aerobic conditions. The potential need for CF bioremediation at the Hanford site has resulted from the large release of carbon tetrachloride (CT) to the subsurface, of...
Resource Type:: Masters Thesis
Full Text:: ammonia was very slow. Microorganisms stimulated on propene and octane showed no ability to transform CF

Transformation of Carbon Tetrachloride and Chloroform by Trichloroethene Respiring Anaerobic Mixed Cultures and Supernatant

Creator:: Vickstrom, Kyle E.
Abstract:: Carbon tetrachloride (CT) and chloroform (CF) were transformed in batch reactor experiments conducted with anaerobic dechlorinating cultures and supernatant (ADC+S) harvested from continuous flow reactors. The Evanite (EV-5L) and Victoria/Stanford (VS-5L) cultures capable of respiring trichloroethene (TCE), 1,2-cis-dichloroethene (cDCE), and vinyl chloride (VC) to ethene (ETH) were grown in continuous...
Resource Type:: Masters Thesis
Full Text:: ) and formate (45 mM; 90 mEq) but no CT or CF. In all experiments, cells and supernatant were

Co-Encapsulation of Slow Release Substrates and Microbial Cultures in Alginate and Gellan Gum Beads to Promote the Co-metabolic Transformation of 1,4-Dioxane and Chlorinate Aliphatic Hydrocarbons

Creator:: Rasmussen, Mitchell T.
Abstract:: Methods were developed for the co-encapsulation of slow release compounds (SRC) with viable microbial cells in alginate and gellan gum hydrogel beads, for the in-situ aerobic cometabolic treatment of groundwater contaminated with mixtures of 1,4-dioxane and chlorinated aliphatic hydrocarbons, which will be referred to as contaminants of concern (CoC). The...
Resource Type:: Masters Thesis
Full Text:: – INTRODUCTION Groundwater contamination is a widespread issue throughout the United States. A report

Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Creator:: Laurance, Jon C.
Abstract:: Chlorinated solvents are common groundwater pollutants that often exist as mixtures resulting from the use of multiple solvents and their transformation into chlorinated daughter products. These sites are often co-contaminated with the solvent stabilizer 1,4-dioxane (1,4-D), which is not treated by traditional chlorinated solvent remediation techniques. Both 1,4-D and chlorinated...
Resource Type:: Masters Thesis
Full Text:: no stimulation of native microorganisms was observed over 50-100 days of incubation. Therefore

Radon-222 as an in situ partitioning tracer for quantifying nonaqueous phase liquid (NAPL) saturations in the subsurface

Creator:: Davis, Brian M.
Abstract:: This study investigated the use of radon-222 as an in situ partitioning tracer for quantifying nonaqueous phase liquid (NAPL) saturations in the subsurface. Laboratory physical aquifer models (PAMs), field experiments, and numerical simulations were used to investigate radon partitioning in static (no-flow) experiments and in single-well, 'push-pull' tests conducted in...
Resource Type:: Dissertation
Full Text:: simulations were used to investigate radon partitioning in static (no-flow) experiments and in single-well

Factors controlling halogenated and nonhalogenated alkene growth substrate range of vinyl chloride-utilizing bacteria

Creator:: Taylor, Anne Elizabeth
Abstract:: This thesis explores the factors limiting the alkene substrate range of the vinyl chloride (VC)-utilizing bacteria, and describes a method for measuring VC transformation in situ. Vinyl fluoride (VF) was evaluated as a surrogate for monitoring aerobic VC-transformation utilizing three isolates, Mycobacterium EE13a, Mycobacterium JS60 and Nocardioides JS614. JS614 grew...
Resource Type:: Dissertation
Full Text:: , and vinyl bromide (VB), whereas propene oxide (PrO) and butene oxide had no effect. Despite EtO and

Inhibition of the ammonia oxidizing bacteria Nitrosomonas europaea by the emerging contaminant triclosan

Creator:: Hughes, Jamie L.
Abstract:: Identifying the inhibition of ammonia oxidizing bacteria (AOB) by emerging organic contaminants is crucial due to the importance of AOB in wastewater treatment, the widespread use of antibacterial agents such as triclosan (TCS) in consumer products, and the sensitivity of N. europaea to inhibitors. Triclosan inhibition of nitrification by AOB...
Resource Type:: Masters Thesis
Full Text:: cell growth. Resistance tests showed no development in resistance to 0.01 ppm TCS over three growth

Aerobic degradation of chlorinated ethenes by Mycobacterium strain JS60 in the presence of organic acids

Creator:: Blatchford, Christina
Abstract:: This study evaluated the potential of the aerobic Mycobacterium strain JS6O to grow on a variety of organic acid substrates, and the possible effects an organic acid would have on the degradation rate of vinyl chloride (VC). A series of batch growth tests were designed to determine the time it...
Resource Type:: Masters Thesis
Full Text:: presence of c-DCE was shown to partially inhibit VC degradation, but had no effect on ethylene degradation

Inhibition, gene expression and modeling of ammonia oxidation in biofilms of Nitrosomonas europaea

Creator:: Lauchnor, Ellen Grace
Abstract:: This dissertation explores the physiology and gene expression of the ammonia-oxidizing bacterium Nitrosomonas europaea in surface-associated bacterial communities, or biofilms. Biofilms of N. europaea were cultivated in drip flow reactors for several weeks and gene expression microarrays were used to detect 240 genes differentially expressed between the mature biofilms and...
Resource Type:: Dissertation
Full Text:: - regulated in both growth states in response to phenol (NE1545-NE1546) while no transcriptional response

ScholarsArchive@OSU

An anaerobic microcosm study of TCE transformation by microbes stimulated from the Gilbert-Mosley site, Wichita, Kansas

Aerobic cometabolism of chloroform by butane and propane grown microorganisms from the Hanford subsurface

Transformation of Carbon Tetrachloride and Chloroform by Trichloroethene Respiring Anaerobic Mixed Cultures and Supernatant

Co-Encapsulation of Slow Release Substrates and Microbial Cultures in Alginate and Gellan Gum Beads to Promote the Co-metabolic Transformation of 1,4-Dioxane and Chlorinate Aliphatic Hydrocarbons

Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Radon-222 as an in situ partitioning tracer for quantifying nonaqueous phase liquid (NAPL) saturations in the subsurface

Factors controlling halogenated and nonhalogenated alkene growth substrate range of vinyl chloride-utilizing bacteria

Inhibition of the ammonia oxidizing bacteria Nitrosomonas europaea by the emerging contaminant triclosan

Aerobic degradation of chlorinated ethenes by Mycobacterium strain JS60 in the presence of organic acids

Inhibition, gene expression and modeling of ammonia oxidation in biofilms of Nitrosomonas europaea

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