The ability of Mycobacterium sp. ELW1, a novel microbe capable of alkene oxidation, to co-metabolize phenanthrene (PHE) was studied. ELW1 was able to completely co-metabolize PHE, at different concentrations below its water solubility limit, in an aqueous environment. The alkene monooxygenases in ELW1, used to initiate oxidation of PHE, were...
Two methanotrophs, M. trichosporium OB3b and M. buryatense 5GB1, were encapsulated using two methods to investigate the potential of methane conversion for biofuel production. Ca-alginate and low melt agarose were used to immobilize the methanotrophs for batch and continuous flow column testing. Varying protein concentrations, residence times, and immobilization methods...
Carbon tetrachloride (CT) and chloroform (CF) were transformed in batch reactor experiments conducted with anaerobic dechlorinating cultures and supernatant (ADC+S) harvested from continuous flow reactors. The Evanite (EV-5L) and Victoria/Stanford (VS-5L) cultures capable of respiring trichloroethene (TCE), 1,2-cis-dichloroethene (cDCE), and vinyl chloride (VC) to ethene (ETH) were grown in continuous...
Identifying the inhibition of ammonia oxidizing bacteria (AOB) by emerging organic contaminants is crucial due to the importance of AOB in wastewater treatment, the widespread use of antibacterial agents such as triclosan (TCS) in consumer products, and the sensitivity of N. europaea to inhibitors. Triclosan inhibition of nitrification by AOB...
This research focused on anaerobic transformation of trichloroethene (TCE), a
groundwater contaminant. The mixed anaerobic Evanite culture (EV) was studied to determine community behavior and composition responses to different electron donors and chloroethene electron acceptors. The potential toxicity from high concentrations of TCE and its daughter product cis-1,2-dichloroethene (cDCE) was...
1,4-dioxane, a probable human carcinogen at low (< 1ppb) concentrations, has emerged as a groundwater contaminant due to its historical use as a stabilizer for the chlorinated solvent 1,1,1-trichloroethane. Aerobic cometabolism, the use of a primary substrate to induce the production of microbial enzymes that fortuitously degrade other compounds, is...
Halogenated organic compounds have had widespread and massive applications in industry, agriculture, and private households, for example, as degreasing solvents, flame retardants and in polymer production. They are released to the environment through both anthropogenic and natural sources. The most common chlorinated solvents present as contaminants include tetrachloroethene (PCE, perchloroethene)...
This study evaluated the potential of the aerobic Mycobacterium strain JS6O to grow on a variety of organic acid substrates, and the possible effects an organic acid would have on the degradation rate of vinyl chloride (VC). A series of batch growth tests were designed to determine the time it...
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3.2 A summary of growth rates determined by fitting an exponential trend line of
x = x0 e kt
Laboratory scale microcosm studies were conducted using site specific groundwater and aquifer solids to assess the feasibility of stimulating indigenous microorganisms in-situ to biologically transform Trichloroethylene (TCE) and its lesser chlorinated daughter products dichloroethylene (DCE) and vinyl chloride (VC). Three different treatments were conducted to determine the best approach for...