Index Catalog // ScholarsArchive@OSU

Vegetative effects of RNAi-suppression of the poplar homolog of LEAFY in the field

Creator:: Jacobson, Jeremy
Abstract:: Due to public and regulatory restrictions, genetic containment technology is critical for research and development of transgenic trees in forestry. A multi-year field trial demonstrated that the RNAi-LFY construct successfully induces sexual sterility in 6K10 poplar (Populus alba) without visible effects on vegetative development. However, its limited number of replicates...
Resource Type:: Undergraduate Thesis and Presentation
Full Text:: 58 Acknowledgements 59 Amy Klocko Steve Strauss Anna Magnuson Cathleen Ma And a special thanks

Overexpression of the GROWTH REGULATING FACTOR 4-GRF-INTERACTING FACTOR 1 Transcription Factor Chimera Modifies Transformation and Regeneration Efficiency in Populus and Eucalyptus

Creator:: Ryan, Nathaniel W.
Abstract:: Transformation is a major bottleneck for genetic engineering and gene editing in forest tree species. This includes most genotypes of Populus and Eucalyptus, which are some of the world’s most widely-cultivated genera of plantation forest trees. To provide new tools for transformation, I tested the transcription factor-protein chimera consisting of...
Resource Type:: Masters Thesis

Functional Characterization and Classification of Genes Essential to Flower Induction, Flower Development, and Seed Development in Populus and Eucalyptus

Creator:: Elorriaga, Estefania
Abstract:: This dissertation consists of four studies of Populus and Eucalyptus biotechnology and genomic science: 1) induction of floral sterility by tapetal expression of the ribonuclease Barnase in Populus; 2) CRISPR Cas9-mediated gene editing targeting LEAFY (LFY) and AGAMOUS (AG) homologs in Populus; 3) induction of floral sterility by CRISPR Cas9...
Resource Type:: Dissertation
Full Text:: work and co-led several of the grants that funded this work along with Dr. Steve Strauss, who oversaw

Host-Induced Gene Silencing in Black Cottonwood for Control of Septoria Canker: Efficacy and Non-Target Impacts

Creator:: Gordon, Michael I.
Abstract:: Septoria canker remains the most important disease of poplars in intensively managed forest plantations. Genetic resistance has long been considered the best way to manage for this disease. Transgenic resistance mediated by RNA silencing against pathogens and pests (HIGS: host-induced gene silencing) has shown promise in other pathosystems but has...
Resource Type:: Dissertation
Full Text:: major advisors Steve Strauss and Jared LeBoldus for their invaluable mentorship, constructive feedback

Sequence Analysis and Vegetative Growth in Transgenic Eucalyptus with CRISPR-Cas9-induced Mutations in the Eucalyptus Homologs of the Floral Genes EMBRYO DEVELOPMENT ARREST 33 (EDA33) and TAPETAL DEVELOPMENT AND FUNCTION1 (TDF1)

Creator:: Nahata, Surbhi S.
Abstract:: An impediment to use of exotic and bioengineered trees in many places is their propensity for spread by pollen and/or seeds. Our laboratory has been using gene editing to induce mutations in floral genes as means to impart stable and reliable genetic containment when this is desirable from social (markets,...
Resource Type:: Masters Thesis
Full Text:: advisor Dr. Steve Strauss, for his constant support and understanding with my thesis. He truly is an

Expression and Function of Populus Homologs to TERMINAL FLOWER 1 Genes: Roles in Onset of Flowering and Shoot Phenology

Creator:: Mohamed, Rozi
Abstract:: We isolated and characterized the expression of two genes from Populus trichocarpa that are homologous to the TERMINAL FLOWER 1 (TFL1) gene from the model annual plant Arabidopsis. In Arabidopsis, overexpression of the TFL1 gene extends the vegetative growth phase, and the homozygous mutant tfl1 allele causes early flowering and...
Resource Type:: Dissertation
Full Text:: . ___________________________________________________________________ Rozi Mohamed, Author ACKNOWLEDGEMENTS I thank my major professors, Steve

Maintenance of normal vegetative growth via attenuation of barnase as an agent of floral tissue ablation in Populus

Creator:: Wei, Hao
Abstract:: To reduce the environmental impacts caused by gene flow from transgenic trees, reproductive sterility genes have been developed that use a floral regulatory element linked to a cell toxin. This results in dysfunction or ablation of floral tissues, causing sterility. However, floral promoters often permit low levels of expression in...
Resource Type:: Masters Thesis
Full Text:: sincerely acknowledges Dr. Steve Strauss for his guidance and advice on this study; Dr. Richard Meilan for

Identification and Functional Characterization of Genes that Regulate Biomass Production, Floral Development, and Leaf Senescence in Populus Species using Genetic Engineering and Bioinformatics Tools

Creator:: Lu, Haiwei
Abstract:: This dissertation is comprised of four studies on different elements of Populus biotechnology and genomic science: 1) growth improvement by recombinant DNA (rDNA) modification of gibberellin (GA) metabolism; 2) gene editing using zinc-finger nucleases (ZFNs); 3) induction of floral sterility by RNA interference (RNAi) of AGAMOUS (AG) gene homologs; and...
Resource Type:: Dissertation
Full Text:: thank Steve Strauss, my major professor and mentor. Along the way Steve’s support, guidance, and

Nuclear and mitochondrial DNA polymorphism and phylogeny in the California closed-cone pines

Creator:: Wu, Junyuan
Abstract:: We studied genetic polymorphism and phylogeny using nuclear random amplified polymorphic DNA markers (RAPDs) and mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) in the three California Closed-Cone Pines: Pinus attenuata Lemm., P. muricata D. Don, and P. radiata D. Don. A total of 343 to 384 trees derived from...
Resource Type:: Masters Thesis

Evaluation of transgenes for stress tolerance, lignin modification, and growth enhancement in poplar

Creator:: Elias, Ani Anna
Abstract:: We evaluated genes previously identified from a large scale functional genomics screen for their potential value to help enhance carbon sequestration in planted trees. We used poplar as a model tree species because of its abundant genetic variation, ease of gene transfer, and availability of large databases for genomic, anatomical,...
Resource Type:: Masters Thesis
Full Text:: project. Dr. Steve Strauss, the Principal Investigator of this study, gave guidance, and support in

ScholarsArchive@OSU

Vegetative effects of RNAi-suppression of the poplar homolog of LEAFY in the field

Overexpression of the GROWTH REGULATING FACTOR 4-GRF-INTERACTING FACTOR 1 Transcription Factor Chimera Modifies Transformation and Regeneration Efficiency in Populus and Eucalyptus

Functional Characterization and Classification of Genes Essential to Flower Induction, Flower Development, and Seed Development in Populus and Eucalyptus

Host-Induced Gene Silencing in Black Cottonwood for Control of Septoria Canker: Efficacy and Non-Target Impacts

Sequence Analysis and Vegetative Growth in Transgenic Eucalyptus with CRISPR-Cas9-induced Mutations in the Eucalyptus Homologs of the Floral Genes EMBRYO DEVELOPMENT ARREST 33 (EDA33) and TAPETAL DEVELOPMENT AND FUNCTION1 (TDF1)

Expression and Function of Populus Homologs to TERMINAL FLOWER 1 Genes: Roles in Onset of Flowering and Shoot Phenology

Maintenance of normal vegetative growth via attenuation of barnase as an agent of floral tissue ablation in Populus

Identification and Functional Characterization of Genes that Regulate Biomass Production, Floral Development, and Leaf Senescence in Populus Species using Genetic Engineering and Bioinformatics Tools

Nuclear and mitochondrial DNA polymorphism and phylogeny in the California closed-cone pines

Evaluation of transgenes for stress tolerance, lignin modification, and growth enhancement in poplar

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