Index Catalog // ScholarsArchive@OSU

Creator:: Wei, Hao
Abstract:: To reduce the environmental impacts caused by gene flow from transgenic trees, reproductive sterility genes have been developed that use a floral regulatory element linked to a cell toxin. This results in dysfunction or ablation of floral tissues, causing sterility. However, floral promoters often permit low levels of expression in...
Resource Type:: Masters Thesis
Full Text:: Attenuation of Barnase as an Agent of Floral Tissue Ablation in Populus Abstract approve1 Steven H. Strauss

Creator:: Wu, Junyuan
Abstract:: We studied genetic polymorphism and phylogeny using nuclear random amplified polymorphic DNA markers (RAPDs) and mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) in the three California Closed-Cone Pines: Pinus attenuata Lemm., P. muricata D. Don, and P. radiata D. Don. A total of 343 to 384 trees derived from...
Resource Type:: Masters Thesis
Full Text:: Polymorphism and Phylogeny in the California Closed-Cone Pines Abstract approved- Steven H. Strauss We

Creator:: Jacobson, Jeremy
Abstract:: Due to public and regulatory restrictions, genetic containment technology is critical for research and development of transgenic trees in forestry. A multi-year field trial demonstrated that the RNAi-LFY construct successfully induces sexual sterility in 6K10 poplar (Populus alba) without visible effects on vegetative development. However, its limited number of replicates...
Resource Type:: Undergraduate Thesis and Presentation

Creator:: Lu, Haiwei
Abstract:: This dissertation is comprised of four studies on different elements of Populus biotechnology and genomic science: 1) growth improvement by recombinant DNA (rDNA) modification of gibberellin (GA) metabolism; 2) gene editing using zinc-finger nucleases (ZFNs); 3) induction of floral sterility by RNA interference (RNAi) of AGAMOUS (AG) gene homologs; and...
Resource Type:: Dissertation
Full Text:: . Abstract approved: ______________________________________________________ Steven H. Strauss This

Creator:: Elorriaga, Estefania
Abstract:: This dissertation consists of four studies of Populus and Eucalyptus biotechnology and genomic science: 1) induction of floral sterility by tapetal expression of the ribonuclease Barnase in Populus; 2) CRISPR Cas9-mediated gene editing targeting LEAFY (LFY) and AGAMOUS (AG) homologs in Populus; 3) induction of floral sterility by CRISPR Cas9...
Resource Type:: Dissertation
Full Text:: : ______________________________________________________ Steven H. Strauss This dissertation consists of four studies of Populus and Eucalyptus

Creator:: Ryan, Nathaniel W.
Abstract:: Transformation is a major bottleneck for genetic engineering and gene editing in forest tree species. This includes most genotypes of Populus and Eucalyptus, which are some of the world’s most widely-cultivated genera of plantation forest trees. To provide new tools for transformation, I tested the transcription factor-protein chimera consisting of...
Resource Type:: Masters Thesis
Full Text:: Steven H. Strauss Transformation is a major bottleneck for genetic engineering and gene

Creator:: Gordon, Michael I.
Abstract:: Septoria canker remains the most important disease of poplars in intensively managed forest plantations. Genetic resistance has long been considered the best way to manage for this disease. Transgenic resistance mediated by RNA silencing against pathogens and pests (HIGS: host-induced gene silencing) has shown promise in other pathosystems but has...
Resource Type:: Dissertation
Full Text:: : ______________________________________________________ Steven H. Strauss Jared M. LeBoldus Septoria canker remains the

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