Index Catalog // ScholarsArchive@OSU

Overexpression of the GROWTH REGULATING FACTOR 4-GRF-INTERACTING FACTOR 1 Transcription Factor Chimera Modifies Transformation and Regeneration Efficiency in Populus and Eucalyptus

Creator:: Ryan, Nathaniel W.
Abstract:: Transformation is a major bottleneck for genetic engineering and gene editing in forest tree species. This includes most genotypes of Populus and Eucalyptus, which are some of the world’s most widely-cultivated genera of plantation forest trees. To provide new tools for transformation, I tested the transcription factor-protein chimera consisting of...
Resource Type:: Masters Thesis
Full Text:: Steven H. Strauss Transformation is a major bottleneck for genetic engineering and gene

Identification and Functional Characterization of Genes that Regulate Biomass Production, Floral Development, and Leaf Senescence in Populus Species using Genetic Engineering and Bioinformatics Tools

Creator:: Lu, Haiwei
Abstract:: This dissertation is comprised of four studies on different elements of Populus biotechnology and genomic science: 1) growth improvement by recombinant DNA (rDNA) modification of gibberellin (GA) metabolism; 2) gene editing using zinc-finger nucleases (ZFNs); 3) induction of floral sterility by RNA interference (RNAi) of AGAMOUS (AG) gene homologs; and...
Resource Type:: Dissertation
Full Text:: . Abstract approved: ______________________________________________________ Steven H. Strauss This

Chloroplast DNA variability and phylogeny in the California closed cone pines

Creator:: Hong, Yong-pyo, 1958-
Abstract:: Chloroplast DNA (cpDNA) variability and phylogeny were studied via analysis of restriction site mutations and DNA sequencing in a complex of three closely related species of pines: Pinus radiata D. Don, P. attenuata Lemm., and muricata D. Don. Genomic DNA from 384 trees representing 20 populations in the complex were...
Resource Type:: Dissertation

PTD : a populus trichocarpa gene with homology to floral homeotic transcription factors

Creator:: Sheppard, Lorraine Anna
Resource Type:: Dissertation
Full Text:: WITH HOMOLOGY TO FLORAL HOMEOTIC TRANSCRIPTION FACTORS Abstract approved: Steven H. Strauss To

Evaluation of transgenes for stress tolerance, lignin modification, and growth enhancement in poplar

Creator:: Elias, Ani Anna
Abstract:: We evaluated genes previously identified from a large scale functional genomics screen for their potential value to help enhance carbon sequestration in planted trees. We used poplar as a model tree species because of its abundant genetic variation, ease of gene transfer, and availability of large databases for genomic, anatomical,...
Resource Type:: Masters Thesis
Full Text:: Poplar. Abstract approved: Steven H. Strauss We evaluated genes previously identified from a

Genetic engineering of disease resistance in poplar : effects of bacterio-opsin over-expression and analysis of a copper-based system for resistance-gene activation

Creator:: Mohamed, Rozi
Abstract:: We tested a synthetic bacterio-opsin (bO) gene derived from Halobacterium halobium for its effect on disease resistance in transgenic poplar hybrids, and assessed a genetic system for controlling expression of this gene. In tobacco the bO gene caused necrotic lesions similar to an induced hypersensitive response (HR), increased levels of...
Resource Type:: Masters Thesis
Full Text:: . Abstract approved: ___ __.,__~'""'T'---'- .. --=~•c:::...J __,._ ____________ _ Steven H. Strauss We

Functional Characterization and Classification of Genes Essential to Flower Induction, Flower Development, and Seed Development in Populus and Eucalyptus

Creator:: Elorriaga, Estefania
Abstract:: This dissertation consists of four studies of Populus and Eucalyptus biotechnology and genomic science: 1) induction of floral sterility by tapetal expression of the ribonuclease Barnase in Populus; 2) CRISPR Cas9-mediated gene editing targeting LEAFY (LFY) and AGAMOUS (AG) homologs in Populus; 3) induction of floral sterility by CRISPR Cas9...
Resource Type:: Dissertation
Full Text:: : ______________________________________________________ Steven H. Strauss This dissertation consists of four studies of Populus and Eucalyptus

Nuclear and mitochondrial DNA polymorphism and phylogeny in the California closed-cone pines

Creator:: Wu, Junyuan
Abstract:: We studied genetic polymorphism and phylogeny using nuclear random amplified polymorphic DNA markers (RAPDs) and mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) in the three California Closed-Cone Pines: Pinus attenuata Lemm., P. muricata D. Don, and P. radiata D. Don. A total of 343 to 384 trees derived from...
Resource Type:: Masters Thesis
Full Text:: Polymorphism and Phylogeny in the California Closed-Cone Pines Abstract approved- Steven H. Strauss We

Vegetative effects of RNAi-suppression of the poplar homolog of LEAFY in the field

Creator:: Jacobson, Jeremy
Abstract:: Due to public and regulatory restrictions, genetic containment technology is critical for research and development of transgenic trees in forestry. A multi-year field trial demonstrated that the RNAi-LFY construct successfully induces sexual sterility in 6K10 poplar (Populus alba) without visible effects on vegetative development. However, its limited number of replicates...
Resource Type:: Undergraduate Thesis and Presentation
Full Text:: Chlorophyll Content* Leaf Specific Weight* (Leaf Mass/Area) Petiole Length Petiole Width Petiole L/W Ratio

Maintenance of normal vegetative growth via attenuation of barnase as an agent of floral tissue ablation in Populus

Creator:: Wei, Hao
Abstract:: To reduce the environmental impacts caused by gene flow from transgenic trees, reproductive sterility genes have been developed that use a floral regulatory element linked to a cell toxin. This results in dysfunction or ablation of floral tissues, causing sterility. However, floral promoters often permit low levels of expression in...
Resource Type:: Masters Thesis
Full Text:: Attenuation of Barnase as an Agent of Floral Tissue Ablation in Populus Abstract approve1 Steven H. Strauss

ScholarsArchive@OSU

Overexpression of the GROWTH REGULATING FACTOR 4-GRF-INTERACTING FACTOR 1 Transcription Factor Chimera Modifies Transformation and Regeneration Efficiency in Populus and Eucalyptus

Identification and Functional Characterization of Genes that Regulate Biomass Production, Floral Development, and Leaf Senescence in Populus Species using Genetic Engineering and Bioinformatics Tools

Chloroplast DNA variability and phylogeny in the California closed cone pines

PTD : a populus trichocarpa gene with homology to floral homeotic transcription factors

Evaluation of transgenes for stress tolerance, lignin modification, and growth enhancement in poplar

Genetic engineering of disease resistance in poplar : effects of bacterio-opsin over-expression and analysis of a copper-based system for resistance-gene activation

Functional Characterization and Classification of Genes Essential to Flower Induction, Flower Development, and Seed Development in Populus and Eucalyptus

Nuclear and mitochondrial DNA polymorphism and phylogeny in the California closed-cone pines

Vegetative effects of RNAi-suppression of the poplar homolog of LEAFY in the field

Maintenance of normal vegetative growth via attenuation of barnase as an agent of floral tissue ablation in Populus

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