The nucleosome appears to be the basic structural unit of the
eukaryotic chromosome. The usual nucleosome preparation, isolated
from a micrococcal nuclease digest of nuclei, is heterogenous in DNA
length and protein composition with some particles containing spacer
DNA and associated protein. This heterogeneity complicated physical
and reconstitution studies. As...
Five forms of chromatin, which represent the in vivo folded forms
of nucleic acid, were isolated and used as the binding substrate for
model intercalating compound, ethidium bromide. For all forms of
chromatin, the affinity, location and structural effects of binding
were examined. On the level of the nucleosome, the...
The structure and transcriptional activity of intranuclear
and isolated chromatin from logarithmically growing
yeast cells has been compared to chromatin from cells which
have entered the stationary phase and ceased growing. Both
chromatins show a similar nucleosomal repeat pattern and a
160 by repeat size when digested with staphlococcal nuclease....