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Filtering by: Subject Chromatin Advisor van Holde, Kensal E.

Structure and function of chromatin : studies at the nucleosome and nuclear matrix levels

Creator:

Shih, Ah Shing

Abstract:

Several levels of eukaryotic chromatin structure have been observed: the nucleosome, the 10 nm and 30 nm fibers and loop domains, apparently attached to the nuclear matrix. In this research, the structure and function of chromatin at two of these levels was investigated, with studies on both nucleosome positioning and...

Resource Type:

Dissertation

Full Text:

Structure and function of chromatin : studies at the
Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatin

Creator:

Dong, Feng

Abstract:

By using immobilized trypsin, I have been able to prepare well-defined, stable trypsinized nucleosomes. The difficulties of lacking of control in the extent of trypsinization, which were encountered in previous studies with the use of free trypsin, have been eliminated. The nucleosomes and oligonucleosomes prepared by immobilized trypsin are suitable...

Resource Type:

Dissertation

Full Text:

"tails" with respect to the structure, stability, and function of chromatin AN ABSTRACT OF THE THESIS
Binding and assembly of H5 (and the globular domain of H5) onto DNA

Creator:

Carter, George John

Abstract:

In order to better characterize linker histone interactions with DNA, avian erythrocyte-specific linker H5 and the trypsin-resistant globular domain of H5 (GH5) were used in DNA binding studies. To begin, H5 displayed a considerably higher binding affinity for DNA than the isolated globular domain (GH5), supporting the importance of the...

Resource Type:

Dissertation

Full Text:

sedimentation studies indicate that H5 in itself was not sufficient to fully compact chromatin fibers; rather