Index Catalog // ScholarsArchive@OSU

Creator:: Durrin, Linda Kay
Abstract:: A rapid virus detection method was developed for Infectious Hemotopoietic Necrosis Virus (IHNV). The virus detection method could be applied to Infectious Pancreatic Necrosis Virus (IPNV) under certain conditions. It was possible to detect IHNV to a lower limit of 1.7 X 10⁴ Tissue Culture Infectious Dose for 50% (TCID₅₀)...
Resource Type:: Masters Thesis

Creator:: Wong, Scott W.
Abstract:: DNA polymerase gamma has been purified over 67,500-fold from human trophoblast tissue. Purification was achieved through successive chromatographies on phosphocellulose, DEAE-cellulose, DNA-cellulose, and high pressure gel exclusion through Fractogel TSK HW55. This preparation yielded a specific activity of 81 units/mg of protein using the preferred template-primer, poly (A)-oligo (dT)₁₂₋₁₃. Analysis...
Resource Type:: Masters Thesis

Creator:: Kurath, Gael
Abstract:: The transcription process of infectious hematopoietic necrosis virus (IHNV), a rhabdovirus of salmonid fish, was studied both in vivo and in vitro. Polyadenylated RNA from IHNV infected-salmon cells was resolved electrophoretically into five bands, one of which was found to contain two mRNA species. The molecular weights and intracellular molar...
Resource Type:: Dissertation

Creator:: Feyereisen-Koener, Josette
Abstract:: Double-stranded cDNA was prepared from infectious hematopoietic necrosis virus mRNA and cloned into the plasmid vector pUC8. A cDNA clone (pG8) containing the entire coding region for the glycoprotein (G-protein) of infectious hematopoietic necrosis virus was selected by hybridization to a ³²P-labeled probe. The restriction map and nucleotide sequence of...
Resource Type:: Dissertation

Creator:: Mourich, Dan V.
Abstract:: The immune systems of various teleost fish have been studied in some detail for the past several decades. One aspect of fish immunity, that of endogenously produced modulating factors, has recently received a great deal of attention. Understanding the functions and roles of endogenous factors that regulate fish immunity is...
Resource Type:: Dissertation

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