Index Catalog // ScholarsArchive@OSU

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Creator:: Bestwick, Richard Keith
Abstract:: This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
Resource Type:: Dissertation

Attempted cloning of T4 phage ribonucleotide reductase

Creator:: Booth, Joseph
Abstract:: Ribonucleotide reductase is an important enzyme in the control of DNA replication within the cell. Ribonucleotide reductase exerts its control through enzymatic reduction of nucleoside diphosphates. The bacteriophage T4 enzyme is an example of the class of iron-requiring reductases which also includes E. coli and mammalian ribonucleotide reductases. The two...
Resource Type:: Masters Thesis

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Creator:: Purohit, Sarla
Abstract:: The dihydrofolate reductase gene of bacteriophate T4 has been investigated at the structural level to elucide the unusual regulation of expression of this gene and possible evolutionary relationships between the ohage and drug resistance factors-encoded dihydrofolate reductases (DHFR). A 1.1-kilobase-pair restriction fragment of the T4 multiple mutant dec8 containing frd,...
Resource Type:: Dissertation

Alternatives to the DNA precursor-synthesizing enzyme complex hypothesis

Creator:: Weitz, Stephen L.
Abstract:: Investigators have been studying an aggregate containing enzymes of deoxyribonucleoside triphosphate biosynthesis in T4-phage infected bacteria. They suggest that it behaves as an organized complex, efficiently channeling DNA precursors to the replication apparatus. Previous work has suggested that about 10 enzyme activities remain associated through several fractionation steps. This multi-enzyme...
Resource Type:: Dissertation

DNA precursor compartmentation in mammalian cells : distribution and rates of equilibration between nucleus and cytoplasm

Creator:: Leeds, Janet M.
Abstract:: A rapid nuclear isolation technique was adapted in order to examine the question of DNA precursor compartmentation in mammalian cells. By using this method a reproducible proportion of the cellular nucleotides remained associated with the isolated nuclei. Examination, at several different cell densities, of exponentially growing HeLa cells showed that...
Resource Type:: Dissertation

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Creator:: Wang, Yeong
Abstract:: Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an enzyme unique to the deoxyribonucleotide metabolism of T-even bacteriophages. To study biochemical and biophysical properties of the enzyme, as well as the interaction of dCMP hydroxymethylase with other DNA precursor biosynthetic enzymes in vitro, availability of large amounts of the enzyme is...
Resource Type:: Dissertation

Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

Creator:: Sargent, Roy Geoffrey
Abstract:: Imbalanced deoxyribonucleoside triphosphate (dNTP) pools are mutagenic for DNA synthesis in both intact cells and cell-free replication systems. Almost certainly, such mutagenesis involves competition between correctly and incorrectly base-paired precursors at replication sites. However, there are certain differences between the intact cell and cell-free systems that do not always allow...
Resource Type:: Dissertation

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

Creator:: Spiro, Craig
Abstract:: Vaccinia virus-infected animal cells have been used to study the interactions between the replication of deoxyribonucleic acid (DNA) and the biosynthesis of its nucleotide precursors. Some antimetabolites that inhibit DNA replication have as their targets enzymes of nucleotide biosynthesis. Furthermore, the disruption of nucleotide metabolism can alter the fidelity of...
Resource Type:: Dissertation

Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

Creator:: Mun, Byung-joo
Abstract:: Although the synthesis of DNA precursors is closely coordinated with DNA replication, it is still not clear whether deoxyribonucleoside triphosphates (dNTPs) influence DNA replication independently of their interactions with DNA polymerase catalytic sites. In an effort to understand the extent to which rate and fidelity of DNA replication are regulated...
Resource Type:: Dissertation

Large subunit of vaccinia cirus ribonucleotide reductase : affinity chromatography-based purification and photoaffinity labeling

Creator:: Warth, Rainer K.
Abstract:: Ribonucleoside diphosphate reductase (RR) from vaccinia virus was recently cloned and overexpressed rn Escherichia coli. The amino acid sequence identities of the small and large subunits between the mouse and the vaccinia virus reductase are approximately 80 and 72 percent, respectively. In addition, vaccinia virus RR displays similar complex allosteric...
Resource Type:: Masters Thesis

ScholarsArchive@OSU

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Attempted cloning of T4 phage ribonucleotide reductase

Bacteriophage T4-coded dihydrofolate reductase : cloning and structural analysis of its gene and organization of flanking regions

Alternatives to the DNA precursor-synthesizing enzyme complex hypothesis

DNA precursor compartmentation in mammalian cells : distribution and rates of equilibration between nucleus and cytoplasm

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

Large subunit of vaccinia cirus ribonucleotide reductase : affinity chromatography-based purification and photoaffinity labeling

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