Sodium dodecyl sulfate polyacrylamide gel electrophoresis and
zymography were used in two separate experiments to determine the tissue
source and type of plasminogen activator (PA) produced by bovine
blastocysts. Twelve-14 d blastocysts were collected at slaughter from
estrous synchronized, superovulated and artificially inseminated Holstein
cows. In Experiment I, blastocysts were...
The effects of several hormones and inducers of intracellular
messengers on plasminogen activator (PA) production and changes in
embryonic size by cultured bovine embryos were evaluated. Day 8 embryos
were cultured in Ham's F-12 with 1.5 mg/ml bovine serum albumin (BSA)
containing different levels of progesterone (P), estradiol -17fl (E₂),...
Effects of phorbol myristate acetate (PMA), dibutyryl cyclic
AMP (dbcAMP), 6-dimethylaminopurine (6-DMAP), okadaic acid
(OA), cycloheximide (CHX), actinomycin D (AcD) and tunicamycin
(TuM) on plasminogen activator (PA) activity and maturation rate
in bovine and porcine oocyte-cumulus cell complexes (BOCC and
POCC, respectively) in vitro were determined. Plasminogen
activator activity was...
Maternally-derived transcripts and proteins support early bovine and ovine embryo development until the 8- to 16-cell stage, at which time embryonic transcripts become essential for continued development. One purported mechanism for the switch from maternal to zygotic control of development (maternal to zygotic genome activation; MZGA) is the appearance of...
During early embryonic development, endodermal cells leave the inner cell mass (ICM) and migrate over an extracellular matrix (ECM), located on the blastocoelic side of the trophectoderm, to form a continuous layer of extraembryonic endoderm. Cell migration events depend on a family of cell surface proteins known as integrins that...