When sulfur dioxide is used in commercial drying of fruits in
combination with low storage temperatures, browning and microbial spoilage
can be inhibited. However, the relationship between moisture level,
storage temperature, and sulfur dioxide content on the color of dried
fruit is not known; therefore the purpose of this research...
Polyphenol oxidase (PPO) from d'Anjou pears was purified and some
molecular properties were studied. The extraction of PPO was
accomplished in the presence of the phenolic binder AG 2X-8 and Triton
X-100. Chlorophyll pigment was removed by chromatography resulting in
a clear, colorless enzyme extract. Three fractions of pear PPO...
Bacillus subtilis α-amylase was used to hydrolyze
starch from peeled and whole potatoes (Solanum tuberosum).
Effect of gelatinization temperature, pH, enzyme concentration,
temperature of hydrolysis and presence of calcium
ions were investigated. Optimum conditions for hydrolysis
were when α-amylase levels were 0.3 percent of the starch
concentration in the presence...
Using phenolic scavengers different methods were examined to determine the optimum conditions for extracting polyphenol oxidase (PPO) from d'Anjou pears. Efficiency of the extraction was evaluated based on browning of the extract, PPO activity per g of pear tissue, absorption scan of 410 nm to -240 nm, and the isozyme...
Peas (Pisum sativum) are an important constituent in the diet
of many people. Peas may be cooked and eaten as green peas shortly
after harvest, stored for short periods in the pods, or, after suitable
treatment, stored for long periods before consumption. Many factors
influence the yield and quality of...
Stability, substrate and inhibitor specificity and electrophoretic
properties of a crude polyphenol oxidase (PPO) preparation extracted
from d'Anjou pears (Pyrus communis L.) were investigated. Levels
of polyvinylpyrrolidone and pH of buffer for extraction were found
to affect the specific activity of the extracted enzyme. An extract
prepared with 1.5 g...
Inhibition of polyphenol oxidase (PPO) by sulfur dioxide (SO₂)
was studied using three different sources of PPO (banana, mushroom,
and pear). Several methods to detect PPO activity were tested due to
SO₂, interference in some of the assays. The method using
2-nitro-5-thiobenzoic acid (TNB) to react with the quinones was...
Polyphenol oxidase (PPO) from strawberries was purified,
partially characterized, and used to study its role in the degradation
of anthocyanin pigments. The extraction method included the use of a
buffered solution containing the phenolic binders Polyclar AT and
Amberlite XAD-4. Addition of Triton X-100 resulted in an increase of
the...