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Effects of physiological state upon nitrosoguanidine-mediated allelic recombination in Saccharomyces cerevisiae

Creator:

Buettner, Michael John

Abstract:

Saccharomyces cerevisiae strain XS-380, a. heteroallelic diploid strain, carries six genetically analyzed loci on the left arm of chromosome VII. Nitrosoguanidine (NMG) at low concentrations was observed to cause allelic recombination (either mitotic recombination or gene conversion) of the heteroallelic loci with low lethality. The NMG-inducible heteroallelic system was used...

Resource Type:

Dissertation
Cell division and DNA synthesis in Saccharomyces cerevisiae following nutritional shiftup

Creator:

Jacobson, Gunnard Kenneth

Abstract:

Nutritional shift-up experiments have revealed many important macromolecular interactions in bacteria. It has been shown that RNA synthesis can be dissociated from protein and DNA synthesis. The rates of protein synthesis were found to be, at a given temperature, strictly dependent on the numbers of mature ribosomes present. DNA synthesis...

Resource Type:

Dissertation
Methionine biosynthesis in Saccharomyces cerevisiae : the one-carbon transfer portion of the pathway

Creator:

Trewyn, Ronny William

Abstract:

The generation of the methyl group of methionine, and is also involved in thymidylic acid and purine biosynthesis. The understanding of the mechanisms and controls involved in one-carbon metabolism is incomplete, and is complicated by the existence of conjugated folate cofactors containing up to seven glutamic acid residues. The information...

Resource Type:

Dissertation
A role for sterols as modulators of membrane fluidity

Creator:

McLean-Bowen, Colleen Ann

Resource Type:

Dissertation