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Filtering by: Subject Silica Contributor advisor sim Rorrer, Gregory L.

2,4,6-trinitrotoluene detection using photoluminescence response of diatom biosilica functionalized with single-chain variable fragment

Creator:

Zhen, Le

Abstract:

A label-free, photoluminescence (PL) based biosensor for 2,4,6-trinitrotoluene (TNT) detection was developed by functionalizing diatom biosiilica with a TNT-specific single-chain variable fragment (scFv). The scFv loading was estimated to be 0.040±0.003 (μg scFv/μg biosilica). In saturated concentration, TNT binding to scFvbiosilica quenched about 13% of its PL. Dose response follows...

Resource Type:

Masters Thesis

Full Text:

. Resonance Energy Transfer- Amplifying Fluorescence Quenching at the Surface of Silica Nanoparticles toward
Biomolecule detection by amplified photoluminescence of germanium doped diatom biosilica

Creator:

Gale, Debra K.

Abstract:

There is significant interest in the fabrication of nano- and micro-structured silica with spatially ordered features, which exhibit enhanced optoelectronic properties with application to the next generation of display devices, semiconductors, and sensors. Currents methods of fabrication employ top down processes which use extremes of temperature, pressure, and power. There...

Resource Type:

Dissertation

Full Text:

silica with spatially ordered features, which exhibit enhanced optoelectronic properties with