Juvenile chinook salmon (Oncorhynchus tshawytscha) were experimentally infected with Renibacterium salmoninarum (Rs), the causative agent of bacterial kidney disease (BKD), to investigate the effects of BKD on three aspects of juvenile salmonid performance: (1) predator avoidance ability; (2) smoltification; and (3) physiological responses to stress. For these experiments, fish with...
This study was undertaken to determine if strontium chloride could be used to create a trans-generational otolith mark in steelhead (Oncorhynchus mykiss). I completed two strontium injection trials and a survey of juvenile steelhead from various steelhead hatcheries. The two trials measured Sr:Ca ratios in otoliths in response to injections...
Although differences between selective pressures in hatcheries
and streams have been theorized to cause genetic divergence between
hatchery and wild salmonids, evidence of this is lacking. This study
was initiated to document the presence or absence of genetic change
in hatchery and wild stocks by characterizing genetic traits in fish...
Sexual maturation and sex differentiation comprise facets of a
common theme: reproduction. The endocrine system regulates many
of the critical physiological processes necessary for reproduction and
offers a framework within which technologies can be developed for
controlling sexual maturation and sex differentiation. The studies
described in this thesis were undertaken...
Stress, including extreme or rapidly changing temperatures, are known to have deleterious effects on fish health and physiology. This thesis examines the combined effects of elevated acclimation temperature and acute handling stress on the number of antibody producing cells, plasma lysozyme concentrations, and the number of pronephric leukocytes in juvenile...
Wild populations of fish are faced with a multitude of stressors, which may include human interaction, toxins, and disease. Benzo(a)pyrene (BaP), a known carcinogen and immunotoxin, has been reported in the stomach contents of juvenile chinook salmon, Oncorhynchus tshawytscha, in urban waterways. We investigated the impact of chronic dietary exposure...
Whole body sex steroid levels of coho salmon,
Oncorhynchus kisutch, were measured via radioimmunoassay
during early development and sexual differentiation.
Steroid profiles of testosterone (T), 11-ketotestosterone
(KT) , androstenedione (A), progesterone (P4) , 17β, -hydroxy-
20β -dihydroprogesterone (DHP) , and 17β -estradiol (E2) were
generated beginning with unfertilized eggs and...
We examined the behavioral and physiological responses of wild
and hatchery-reared cutthroat trout Oncorhynchus clarki subjected to
a single electroshock, electroshock plus marking, and multiple
electroshocking in natural and artificial streams. In a natural
stream, trout released after capture by electrofishing and marking
showed distinct behavioral changes: fish immediately sought...
Various aspects of the energetic response to handling stress in juvenile
coho salmon (Oncorhynchus kisutch) were examined. Fish were subjected to
four different handling stressors in a Blazka-style respirometer. Stressed fish
had rates of oxygen consumption that were higher than controls. The
magnitude of the increase ranged from 139 to...