Index Catalog // ScholarsArchive@OSU

Host-Induced Gene Silencing in Black Cottonwood for Control of Septoria Canker: Efficacy and Non-Target Impacts

Creator:: Gordon, Michael I.
Abstract:: Septoria canker remains the most important disease of poplars in intensively managed forest plantations. Genetic resistance has long been considered the best way to manage for this disease. Transgenic resistance mediated by RNA silencing against pathogens and pests (HIGS: host-induced gene silencing) has shown promise in other pathosystems but has...
Resource Type:: Dissertation

Overexpression of the GROWTH REGULATING FACTOR 4-GRF-INTERACTING FACTOR 1 Transcription Factor Chimera Modifies Transformation and Regeneration Efficiency in Populus and Eucalyptus

Creator:: Ryan, Nathaniel W.
Abstract:: Transformation is a major bottleneck for genetic engineering and gene editing in forest tree species. This includes most genotypes of Populus and Eucalyptus, which are some of the world’s most widely-cultivated genera of plantation forest trees. To provide new tools for transformation, I tested the transcription factor-protein chimera consisting of...
Resource Type:: Masters Thesis

Sequence Analysis and Vegetative Growth in Transgenic Eucalyptus with CRISPR-Cas9-induced Mutations in the Eucalyptus Homologs of the Floral Genes EMBRYO DEVELOPMENT ARREST 33 (EDA33) and TAPETAL DEVELOPMENT AND FUNCTION1 (TDF1)

Creator:: Nahata, Surbhi S.
Abstract:: An impediment to use of exotic and bioengineered trees in many places is their propensity for spread by pollen and/or seeds. Our laboratory has been using gene editing to induce mutations in floral genes as means to impart stable and reliable genetic containment when this is desirable from social (markets,...
Resource Type:: Masters Thesis

Functional Characterization and Classification of Genes Essential to Flower Induction, Flower Development, and Seed Development in Populus and Eucalyptus

Creator:: Elorriaga, Estefania
Abstract:: This dissertation consists of four studies of Populus and Eucalyptus biotechnology and genomic science: 1) induction of floral sterility by tapetal expression of the ribonuclease Barnase in Populus; 2) CRISPR Cas9-mediated gene editing targeting LEAFY (LFY) and AGAMOUS (AG) homologs in Populus; 3) induction of floral sterility by CRISPR Cas9...
Resource Type:: Dissertation

Identification and Functional Characterization of Genes that Regulate Biomass Production, Floral Development, and Leaf Senescence in Populus Species using Genetic Engineering and Bioinformatics Tools

Creator:: Lu, Haiwei
Abstract:: This dissertation is comprised of four studies on different elements of Populus biotechnology and genomic science: 1) growth improvement by recombinant DNA (rDNA) modification of gibberellin (GA) metabolism; 2) gene editing using zinc-finger nucleases (ZFNs); 3) induction of floral sterility by RNA interference (RNAi) of AGAMOUS (AG) gene homologs; and...
Resource Type:: Dissertation

Vegetative effects of RNAi-suppression of the poplar homolog of LEAFY in the field

Creator:: Jacobson, Jeremy
Abstract:: Due to public and regulatory restrictions, genetic containment technology is critical for research and development of transgenic trees in forestry. A multi-year field trial demonstrated that the RNAi-LFY construct successfully induces sexual sterility in 6K10 poplar (Populus alba) without visible effects on vegetative development. However, its limited number of replicates...
Resource Type:: Undergraduate Thesis and Presentation

Dispersed repetitive sequences in the chloroplast genome of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco)

Creator:: Tsai, Ching-Hsiu
Abstract:: Chloroplast genomes of conifers are unusual in a number of respects, including the possession of much repetitive DNA. By studying the distribution and sequences of dispersed repetitive DNA, I hoped to gain insight into the mechanisms of chloroplast genome evolution. I used restriction mapping and DNA sequencing to characterize dispersed...
Resource Type:: Masters Thesis

PTD : a populus trichocarpa gene with homology to floral homeotic transcription factors

Creator:: Sheppard, Lorraine Anna
Resource Type:: Dissertation

Nuclear and mitochondrial DNA polymorphism and phylogeny in the California closed-cone pines

Creator:: Wu, Junyuan
Abstract:: We studied genetic polymorphism and phylogeny using nuclear random amplified polymorphic DNA markers (RAPDs) and mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) in the three California Closed-Cone Pines: Pinus attenuata Lemm., P. muricata D. Don, and P. radiata D. Don. A total of 343 to 384 trees derived from...
Resource Type:: Masters Thesis

Maintenance of normal vegetative growth via attenuation of barnase as an agent of floral tissue ablation in Populus

Creator:: Wei, Hao
Abstract:: To reduce the environmental impacts caused by gene flow from transgenic trees, reproductive sterility genes have been developed that use a floral regulatory element linked to a cell toxin. This results in dysfunction or ablation of floral tissues, causing sterility. However, floral promoters often permit low levels of expression in...
Resource Type:: Masters Thesis

ScholarsArchive@OSU

Host-Induced Gene Silencing in Black Cottonwood for Control of Septoria Canker: Efficacy and Non-Target Impacts

Overexpression of the GROWTH REGULATING FACTOR 4-GRF-INTERACTING FACTOR 1 Transcription Factor Chimera Modifies Transformation and Regeneration Efficiency in Populus and Eucalyptus

Sequence Analysis and Vegetative Growth in Transgenic Eucalyptus with CRISPR-Cas9-induced Mutations in the Eucalyptus Homologs of the Floral Genes EMBRYO DEVELOPMENT ARREST 33 (EDA33) and TAPETAL DEVELOPMENT AND FUNCTION1 (TDF1)

Functional Characterization and Classification of Genes Essential to Flower Induction, Flower Development, and Seed Development in Populus and Eucalyptus

Identification and Functional Characterization of Genes that Regulate Biomass Production, Floral Development, and Leaf Senescence in Populus Species using Genetic Engineering and Bioinformatics Tools

Vegetative effects of RNAi-suppression of the poplar homolog of LEAFY in the field

Dispersed repetitive sequences in the chloroplast genome of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco)

PTD : a populus trichocarpa gene with homology to floral homeotic transcription factors

Nuclear and mitochondrial DNA polymorphism and phylogeny in the California closed-cone pines

Maintenance of normal vegetative growth via attenuation of barnase as an agent of floral tissue ablation in Populus

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