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Filtering by: Committee Member Barofsky, Douglas Degree Field Chemistry Language English [eng] Rights Statement Copyright Not Evaluated

Mass spectrometric analysis of bovine neurofilament proteins NF-L, NF-M, and NF-H : peptide mapping, phosphorylation and alkylation site identification

Creator:

Mixon, April E.

Abstract:

Neurofilament proteins are intermediate filaments found in the neuronal cytoskeleton. Phosphorylation of these proteins is considered important for the assembly and stability of the filaments. Accurate molecular weights have been difficult to measure, largely because the high degree of phosphorylation results in M[subscript r]'S that are significantly greater than dictated...

Resource Type:

Masters Thesis
Mass spectrometric studies on peptides and proteins : conformations of Escherichia coli Thioredoxin and its alkylated adducts studied by hydrogen/deuterium exchange and HPLC-electrospray ionization mass spectrometry

Creator:

Kim, Moo-young

Abstract:

E. coli thioredoxin (TRX) was modified by the episulfonium ion derived from S-(2-chloroethyl)glutathione (CEG) or S-(2-chloroethyl)cysteine (CEC). The alkylation site was located at Cys-32, which was confirmed by tandem mass spectrometry. Two forms of native TRX, Oxi- and Red-TRX, and two modified TRXs, GS- and Cys-TRX, were examined by hydrogen/deuterium...

Resource Type:

Dissertation
Novel monolithic columns for microscale liquid chromatography and capillary electrochromatography

Creator:

Chirica, Gabriela S.

Abstract:

The challenges prompted by miniaturization of chromatographic systems have led scientists to explore new avenues in the synthesis of stationary phases. Packed capillary columns have found limited application in routine analysis due to a number of technical difficulties, most of which are attributable to the frits. New column designs insure...

Resource Type:

Dissertation

ScholarsArchive@OSU

Mass spectrometric analysis of bovine neurofilament proteins NF-L, NF-M, and NF-H : peptide mapping, phosphorylation and alkylation site identification

Mass spectrometric studies on peptides and proteins : conformations of Escherichia coli Thioredoxin and its alkylated adducts studied by hydrogen/deuterium exchange and HPLC-electrospray ionization mass spectrometry

Novel monolithic columns for microscale liquid chromatography and capillary electrochromatography

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