E. coli thioredoxin (TRX) was modified by the episulfonium ion derived from S-(2-chloroethyl)glutathione (CEG) or S-(2-chloroethyl)cysteine (CEC). The alkylation site was located at Cys-32, which was confirmed by tandem mass spectrometry. Two forms of native TRX, Oxi- and Red-TRX, and two modified TRXs, GS- and Cys-TRX, were examined by hydrogen/deuterium...
The challenges prompted by miniaturization of chromatographic systems have led scientists to explore new avenues in the synthesis of stationary phases. Packed capillary columns have found limited application in routine analysis due to a number of technical difficulties, most of which are attributable to the frits. New column designs insure...
Neurofilament proteins are intermediate filaments found in the neuronal cytoskeleton. Phosphorylation of these proteins is considered important for the assembly and stability of the filaments. Accurate molecular weights have been difficult to measure, largely because the high degree of phosphorylation results in M[subscript r]'S that are significantly greater than dictated...