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Filtering by: Has journal Plant, Cell & Environment Remove constraint Has journal: Plant, Cell & Environment Committee Member Chang, Jeff Remove constraint Committee Member: Chang, Jeff Degree Field Microbiology Remove constraint Degree Field: Microbiology

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  • Secreted Agrobacterium rhizogenes Effector Protein GALLS Interacts with Arabidopsis Host Proteins and Stimulates Agrobacterium-mediated Transformation

    Creator:
    Wei, Wei
    Abstract:
    Agrobacterium species transform plant cells by targeting a portion of plasmid-encoded bacterial DNA to the host nucleus. Genetic transformation by A. tumefaciens and A. rhizogenes requires secreted effector proteins. The tumor-inducing (Ti) plasmid from virulent A. tumefaciens encodes VirE2, a secreted single-stranded DNA-binding protein required for efficient transformation of hosts....
    Resource Type:
    Dissertation
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    Page 1.1 General steps in gene transfer between Agrobacterium (left) & plant cell (right

  • Quorum sensing gene regulation in Pseudomonas aeruginosa

    Creator:
    Gupta, Rashmi
    Abstract:
    Pseudomonas aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals such as those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell communication mechanism known as quorum sensing (QS) to coordinate virulence gene expression and biofilm formation. It has three interconnected QS systems, namely...
    Resource Type:
    Dissertation
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    those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell

  • Impacts of microbial chemical interactions on marine plankton physiology

    Creator:
    Moore, Eric R.
    Abstract:
    The bioavailability of chemical compounds in the marine environment fundamentally influences the growth and physiology of microorganisms. Organic and inorganic chemicals that are produced by some marine plankton can be consumed by other plankton for energy production, growth, or to initiate essential physiological processes. Cultures of the diatom Thalassiosira pseudonana,...
    Resource Type:
    Dissertation
    Full Text:
    bioavailability of chemical compounds in the marine environment fundamentally influences the growth and

  • Genome-enabled investigation of the minimal growth requirements andphosphate metabolism for Pelagibacter marine bacteria

    Creator:
    Carini, Paul J.
    Abstract:
    Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...
    Resource Type:
    Dissertation
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    that Ca. P. ubique has an apparent requirement for 10.2 attomoles Pi cell-1 and Pelagibacter sp. str

  • Social Evolution and Regulatory Architecture of Pseudomonas aeruginosa Quorum Sensing

    Creator:
    Asfahl, Kyle L.
    Abstract:
    Cell-cell communication in bacteria is understood to facilitate the coordination of population-wide cooperative behavior in the form of concerted gene expression. The opportunistic pathogen Pseudomonas aeruginosa uses such a communication mechanism to regulate a large group of genes important to virulence strategies in this bacterium. This general mechanism of communication...
    Resource Type:
    Dissertation
    Full Text:
    Cell-cell communication in bacteria is understood to facilitate the coordination of population-wide

  • Cooperation and conflict in Pseudomonas aeruginosa quorum sensing

    Creator:
    Wilder, Cara N.
    Abstract:
    Pseudomonas aeruginosa is an environmental bacterium as well as an opportunistic pathogen that primarily infects immunocompromised individuals, including those suffering from cystic fibrosis. The density-dependent regulation of gene expression via cell-to-cell communication, also termed quorum sensing (QS), is an important virulence determinant in this organism. Generally, P. aeruginosa uses three...
    Resource Type:
    Dissertation
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    freely diffuses across the cell membrane into the surrounding environment. Once a critical autoinducer

  • The Fitness Costs and Regulatory Strategies of Cooperative Siderophore Production in Pseudomonas

    Creator:
    Sexton, David
    Abstract:
    Cooperative behaviors in bacteria are increasingly appreciated for their relevance to microbial ecology and utility as model systems for social evolution. One example is the secretion of siderophores, a structurally diverse group of compounds that chelate extracellular iron. Siderophore production is considered cooperative because the benefits can be shared with...
    Resource Type:
    Dissertation
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    from the extracellular environment. This is considered cooperation because the benefits are shared

  • Advances in Freshwater Cyanobacterial Genomics

    Creator:
    Brown, Nathaniel M.
    Abstract:
    Limnology is undergoing a transition to high-throughput -omic analysis of freshwater bacterial communities. An important first step in making the transition is to characterize several genomes that can be used as references to guide metagenome assembly and analysis. Here I characterize four new freshwater cyanobacterial genomes, a pair of lake...
    Resource Type:
    Dissertation
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    genome nearly a year prior to establishing the culture. It has been under selection in the environment