RNA interference pathways can involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs, including transacting siRNAs (tasiRNAs), arise from transcripts targeted by some microRNAs (miRNAs). In the case of TAS3 tasiRNA formation, ARGONAUTE7 (AGO7)-miR390 complexes interact with primary transcripts at two sites, resulting in recruitment...
Batch cultures of Candidatus Pelagibacter ubique were grown under iron-, organosulfur-, and nitrogen-limiting conditions to understand how this ubiquitous marine bacterium responds to and interacts with environments where growth is limited by the availability of these nutrients. Global gene expression was monitored using microarrays and quantitative mass spectrometry to observe...
miRNA-guided cleavage initiates entry of primary transcripts into the trans-acting siRNA (tasiRNA) biogenesis pathway involving RNA-DEPENDENT RNA POLYMERASE6 (RDR6), DICER-LIKE 4 (DCL4), and SUPPRESSOR OF GENE SILENCING3 (SGS3). Arabidopsis thaliana TAS1 and TAS2 families yield tasiRNA that form through miR173- guided initiation cleavage on the 5’ side of the siRNA-generating...