Energy production and consumption is a major environmental and economic concern in the world today. Biofuel produced from lignocellulosic biomass is being explored as an alternative energy source to petroleum. Lignocellulosic biomass is comprised of cellulose, hemicellulose, and lignin. To produce biofuels, lignocellulosic biomass must be broken down to expose...
In order to reduce the cost of lignocellulosic ethanol production, a model was previously developed to predict the amount of fermentable sugar released based on parameters concerning both biomass and enzymes. To validate this model, enzymatic hydrolysis must be carried out using individual purified cellulase enzymes. Endo-1,4-β-D-glucanase I (EG I),...