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Filtering by: Creator Elliot, Alexa B. Remove constraint Creator: Elliot, Alexa B. Committee Member Fowler, John Remove constraint Committee Member: Fowler, John Language English [eng] Remove constraint Language: English [eng]

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  • Regulation of phospholipase C-beta isozymes by calmodulin

    Creator:
    McCullar, Jennifer Star
    Abstract:
    Phospholipase C-Jβ (PLCβ) is an important effector enzyme in G-protein-coupled signaling pathways. Activation of PLCβ by Gα and Gβy subunits has been fairly well characterized, but little is known about other protein interactions that may also regulate PLCβ function. A yeast two-hybrid screen of a mouse brain cDNA library with...
    Resource Type:
    Dissertation
    Full Text:
    anisotropy using Alexa-CaM. 83 3.4 Effect of CaM on catalytic activity of PLC 3

  • Dissecting the cytoskeletal interaction during cytokinesis and the role of myosin II motors on homologous chromosomes

    Creator:
    Tseng, Kuo-Fu
    Abstract:
    Prior to cell cleavage, cytokinetic proteins are recruited into the nascent actomyosin contractile ring. Interactions between spindle microtubules and the cell cortex play a critical role in this recruitment. However, direct evidence for physical interaction between microtubules and the cortex has been lacking. Here we reveal the physical connection between...
    Resource Type:
    Dissertation
    Full Text:
    was injected with Alexa-488 tagged tubulin to label microtubules (green) and Rhadomine-phollodin to

  • In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP

    Creator:
    Venkataraman, Anand
    Abstract:
    Studies using the pluripotent embryonic carcinoma cell line, P19, as a retinoic acid (RA)-responsive model system have been instrumental towards our understanding of the RA-dependent signaling pathways in development and homeostasis. Grp1-associated scaffold protein (GRASP; also known as Tamalin) was first identified by our group, as a gene robustly induced...
    Resource Type:
    Dissertation
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    accessory proteins is essential for activation of intracellular signaling cascades (20,21). (b) PDZ

  • Regulation by male-specific fruitless of neural circuitry used during courtship and copulation behavior in Drosophila melanogaster

    Creator:
    Latham, Kristin Lynn
    Abstract:
    Courtship and copulation behaviors in Drosophila melanogaster males are regulated by sex-specific products from the gene fruitless (fru). Male-specific FRU proteins (FRU[superscript M]) are putative transcription factors of the BTB-ZnF family that likely act by controlling development and maintenance of the neural circuitry used during male sexual behavior. However, which...
    Resource Type:
    Dissertation
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    ebony (e) and black (b) both alter levels of aggression, it is thought through regulation of levels

  • Computational Approaches for Understanding Combinatorial Gene Regulation

    Creator:
    Ansariola, Mitra
    Abstract:
    Gene regulation is a complex mechanism that controls the spatial and temporal expression of genes in a living cell. My dissertation studies focus on two problems. First, tissue-specific gene expression prediction from DNA sequence and chromatin state, and second, the accurate discovery of small over-represented regulatory circuits in gene regulatory...
    Resource Type:
    Dissertation

  • Mechanisms for the interaction of environmental mycobacteria with host cells

    Creator:
    Harriff, Melanie J.
    Abstract:
    Environmental mycobacteria are important opportunistic pathogens for many hosts, including humans, cattle, and fish. Two well-studied species are Mycobacterium avium subsp. avium, a significant cause of disseminated bacterial disease in patients with AIDS, and Mycobacterium avium subsp. paratuberculosis, the cause of Johne’s disease in cattle. Many other species that are...
    Resource Type:
    Dissertation
    Full Text:
    expression of genes in M. avium over- 44 expressing A) MAV_3679, or B) MAV_5138, compared to wild-type

  • Molecular genetic analysis of fruit development in tomato (Lycopersicon esculentum, Mill.)

    Creator:
    Balbi, Virginia
    Abstract:
    Plant hormones regulate progression through the different phases of plant development in response to endogenous and environmental cues. Gene expression studies, as well as genetic and molecular analysis of mutants with altered hormonal responses, are two common approaches to elucidate hormone response pathways. This dissertation compares ovary and fruit development...
    Resource Type:
    Dissertation
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    ........................................................................................ 86 3.3.4 Expression analysis of CDKA and cyclin B genes in dgt and wild-type ovaries