The study was undertaken to determine if a university-level
preliminary class concentrating upon the formalism and techniques
germane to success in science would serve to improve minority
academic performance, minority here being defined to include certain
Asian Americans, Black Americans, Native Americans, Spanish
surnamed Americans, and those white Americans who...
Four experimental groups were designed to examine the effects of a "greens" dietary supplement (wheat clippings), as well as individual versus group-housing, on the adrenal and reproductive physiology of Microtus canicaudus. Organ culture of adrenal glands, thin-layer chromatography, and fluorescence analysis of steroid-containing medium were used to quantitatively assess adrenal...
This study was conducted to investigate the involvement of selenium and glutathione peroxidase in
prostaglandin biosynthesis by the ram vesicular gland.
Vesicular glands were collected from healthy intact
rams covering a wide range of selenium nutrition and were
analyzed for selenium concentrations, glutathione
peroxidase activities and levels of prostaglandin E₂...
The interaction of skeletal muscle phosphofructokinase
with a variety of acidic proteins including calmodulin and
troponin C and with nucleic acids (RNA and DNA) is manifest
in a reversible, time-dependent loss of catalytic activity.
The inactivation is affected by a number of factors.
Substrates (fructose-6-phosphate and ATP), positive
effectors (fructose-2,6-bisphosphate,...
The consistent application of phosphatase inhibitors and a novel final purification step using a connected series of DE-51, DE-52, and DE-53 anion exchange chromatography columns facilitate the preparation of electrophoretically homogeneous sub-pupulations of rabbit muscle phosphofructokinase which differ in their catalytic properties and endogenous covalent phosphate contents. A band of...
5'-Deoxy-5'-methylthioadenosine phosphorylase catalyzes
the phosphorolytic cleavage of 5'-deoxy-5'-methylthioadenosine
to form 5'-deoxy-5'-methylthioribose-1-phosphate and
adenine in mammalian cells and plays an important role in
methionine and purine salvage. The enzyme is abundant in
normal tissues and in cell lines derived from normal cells.
However, several malignant tissues and cell lines have been...
Four experiments were conducted to determine oxytocin concen tration of bovine luteal tissue at different stages of the estrous cycle and to study the effects of prostaglandins F₂α (PGF₂α), E₂ (PGE₂), luteinizing hormone (LH), cycloheximide, colchicine and cytochalasin B on oxytocin synthesis and release in vitro. In experiment 1, luteal...
The purpose of this study was to determine the effects
of providing behavioral objectives prior to instruction on
the achievement of students in a one-semester college
general chemistry course and to determine the effect of
providing behavioral objectives of selected chemistry
experiments in the acquisition of achievement associated
with laboratory...
Ribosomes are multicomponent macromolecular particles and
are essential for the survival of cells in all organisms. The ribosome's
universal function is to catalyze polypeptide synthesis through
translation of mRNA transcripts. Ribosomes from Escherichia coli,
eubacterial organisms, have a sedimentation coefficient of 70S and are
composed of 30S and 50S ribonucleoprotein...
There is plenty of evidence from epidemiology studies
supporting a link between certain components in the human
diet and cancer incidence. It is estimated that 3-4 million
annual cases of cancer could be prevented worldwide just by
changing dietary habits. In parts of the world where
vegetables and fruits constitute...
The objective of this study was to further determine the distribution of
selenoprotein W (SeW) in tissues from rats and sheep fed different selenium levels and to
search for the possible functions of this protein. In the rat study a total of 28 rat tissues
were examined and SeW was...
Indole-3-carbinol (I3C), the most abundant metabolite of glucobrassicins, is
found in cruciferous vegetables such as broccoli, Brussels sprouts, cabbage and
cauliflower. I3C has been shown to have anticarcinogenic activity against many
chemical carcinogens in multiple target organs in animal models. However, the
anticarcinogenic activity of I3C is considered indirect since...
This study was designed to investigate the rate of pH decline, changes in protein solubility and in the water-holding capacity (WHC) of beef muscles subjected to pre-rigor electrical stimulation. Samples for the experiment were obtained from animals slaughtered at the Meat Science Laboratory, Oregon State University. Nine good quality steers...
The purpose of this study was, first, to investigate whether there
is a vitamin B-6 requirement for carnitine synthesis and, second, to investigate
the effect of fasting on vitamin B-6 metabolism. An experimental
group of 72 rats (6 per group) were fed either a vitamin B-6 deficient
diet (-B6) (ad...
Chipping varieties and model systems were used to determine the role of potato
constituents on chip color. Composition was evaluated by HPLC and chip color
measured using a ColorQuest colorimeter. Reducing sugar (RS) content did not
completely explain color quality when present in low concentrations (<60 mg/lOOg).
Levels of ascorbic...
Normal human skin fibroblast cells were used to study the effect
of zinc supplementation of the media on cell growth and the competitive
binding activity of low density lipoprotein (LDL). Cells were
grown in the media containing Dulbecco's Modified-Eagle Medium (DMEM),
5% (v/v) fetal calf serum (FCS), and various levels...
Edible coatings controlling preservative migration from
surface to food bulk could inhibit surface microbial growth
which is often the main cause of spoilage for many food
products. In this project we focused our attention upon
methylcellulose, hydroxypropyl methylcellulose, and chitosan as
the structural component for such edible films. These films...
Commercially bottled, vitamin C-fortified, cranberry
juice cocktail and cranberry-apple (cranapple) juice
drinks were stored at 5°C under simulated home-use
conditions. Ascorbic acid (AA) and dehydroascorbic acid
(DHA) levels were evaluated over a period of seven days by
high performance liquid chromatography (HPLC). Total
vitamin C content was also determined by...
The effect of source of dietary carboyhydrate upon the concentration
and distribution of phospholipids in the fractions of human
blood was studied. Three healthy women received diets which contained
16% of the calories as protein, 40% as fat, and 44% as carbohydrate.
During the four dietary periods of six days...
Current dietary recommendations have placed increasing emphasis on dietary fat
as an important element to decrease risk of cardiovascular disease (CVD). Although total
fat and the fatty acid composition of diets influence the risk of CVD, the optimal amounts
of different fatty acids are not well defined, especially if n-6...
Anserine and carnosine are water-soluble dipeptides that have antioxidant
properties and are found in the skeletal muscle of mammals and fishes. These
dipeptides are removed through the washing process in surimi production. The
objective of this research was to determine the concentration of anserine and
carnosine in surimi wash water...
Alcoholic off-flavor and accompanying volatile compounds produced by bananas (Cavendishii spp) held at 20°C and subjected to aerobic (air control treatment) or anaerobic conditions (nitrogen treatment) were compared by solid phase micro extraction (SPME), a newly developed method for volatile separation. In general, volatile production was suppressed under anaerobic conditions...